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Product Details
RP-99141
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EAEVRPKIPGSREAAENDEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEER
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (42%), Rat (42%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60811. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR and RyR. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310032K21Rik; homolog of mouse skeletal muscle sarcoplasmic reticulum protein JP-45; JP-45; Junctional sarcoplasmic reticulum protein 1; Junctional SR; Junctional-face membrane protein of 45 kDa homolog; skeletal muscle sarcoplasmic reticulum protein JP-45
Gene Aliases: JP-45; JP45; JSRP1
UniProt ID: (Human) Q96MG2
Entrez Gene ID: (Human) 126306
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