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Product Details
RP-88096
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human LAG3, amino acids Met1-Leu450 (Accession # NP_002277.4) with a C-terminal mouse IgG1 Fc region
Tag
Fc-tag
Molecular weight
72.58 kDa
Class
Recombinant
Type
Protein
Purity
≥90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized LAG3 Protein, Human, Recombinant (ECD, mFc Tag) at 2 µg/mL (100 µL/well) can bind Anti-LAG3 (BMS)-IgG4 Antibody (Relatlimab), the EC50 is 8-40 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 15% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (330 µL) be added to the vial to prepare a stock solution of 0.15 mg/mL.
Concentration is measured by UV.
Target Information
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD223; LAG-3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3
Gene Aliases: CD223; FDC; LAG3
UniProt ID: (Human) P18627
Entrez Gene ID: (Human) 3902
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