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Product Details
RP-106261
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
QLASSLSSQKEVAATEEDVTRLPSPTSPFSSLSQDQAATSKATLSSTSGLDLMSESGEGEISPQREVSRSQDQFSDMRISINQTPGKSLDFGFTIKWDIPGIFVASVEAGSPAEFSQLQVDDEIIAINNTKFSYNDSKEWEEA
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (73%), Rat (73%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-111307. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The LIM-only (LMO) proteins are nuclear factors characterized by a conserved LIM domain. The LIM domain contains a cysteine-rich zinc-binding motif, present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system. The deduced LMO7 protein is comprised of 1,349 amino acid residues, contains a characteristic zinc finger domain and a 3-prime UTR which possesses a short interspersed nucleotide element (SINE). RT-PCR detects predominant expression of LMO7 in heart, lung, skeletal muscle, and kidney, moderate expression in liver, ovary, brain, pancreas, and testis, and little to no expression in spleen. Research indicates that LMO7 is an afadin- and alpha-actinin-binding protein that connects the nectin-afadin and E-cadherin-catenin systems through alpha-actinin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: F-box only protein 20; F-box protein Fbx20; LIM domain only 7 protein; LIM domain only protein 7; LMO-7; LOMP; LOMP protein; RP11-332E3.2; zinc-finger domain-containing protein
Gene Aliases: FBX20; FBXO20; KIAA0858; LMO7; LOMP
UniProt ID: (Human) Q8WWI1
Entrez Gene ID: (Human) 4008
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