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Invitrogen
Human Laminin alpha-3 (aa 2002-2109) Control Fragment Recombinant Protein
Product Details
RP-89598
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
LLNRIRTWQKTHQGENNGLANSIRDSLNEYEAKLSDLRARLQEAAAQAKQANGLNQENERALGAIQRQVKEINSLQSDFTKYLTTADSSLLQTNIALQLMEKSQKEYE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (74%), Rat (74%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-52665. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Laminin is a non-collagenous protein of basement membranes. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400 kD) and three short arms (200 kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BM600 150kD subunit; E170; Epiligrin; Epiligrin 170 kDa subunit; epiligrin alpha 3 subunit; Epiligrin subunit alpha; kalinin 165kD subunit; Kalinin subunit alpha; LAM3, alpha-3 subunit; laminin 5, alpha-3 subunit; laminin A3; Laminin subunit alpha-3; laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin); Laminin-5; laminin-5 alpha 3 chain; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; nicein 150kD subunit; Nicein subunit alpha
Gene Aliases: BM600; E170; LAMA3; LAMNA; LOCS
UniProt ID: (Human) Q16787
Entrez Gene ID: (Human) 3909
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