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Human/Mouse/Rat BMP-2 Recombinant Protein, PeproTech®

Human/Mouse/Rat BMP-2 Protein in Functional Assay (Functional)

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Human/Mouse/Rat BMP-2 Protein (120-02C-10UG) in Functional

Bioassay analysis of Human/Mouse/Rat BMP-2 Recombinant Protein, PeproTech® (Product # 120-02C-1MG).

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Product Details

120-02C-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 17 publications 17 publications

Product Specifications

Species

Human, Mouse, Rat

Published species

Human, Mouse

Expression System

CHO cells

Amino acid sequence

QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR

Molecular weight

25.8 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 40-100 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human/Murine/Rat BMP-2 derived from CHO cells is a homodimeric glycoprotein that consists of two 114 amino acid polypeptide chains linked by a single disulfide bond. Due to glycosylation, CHO cell-derived Human/Murine/Rat BMP-2 migrates at an apparent molecular weight of approximately 28-29 kDa by SDS-PAGE analysis under non-reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (Hogan 1996, Reddi 1998 and Francis-West et al. 1999). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration. Lovostatin increases bone formation by turning on the bmp-2 gene (Mundy et al. 1999). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (Macias et al. 1999, Lecanda et al. 1997). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (Hogan 1996, Dale and Wardle 1999). BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (Massague 1994, Derynck 1997, Attisano 1993).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of PDGFRA⁺ cells and a CD55⁺ PDGFRA^Lo fraction in the gastric mesenchymal niche.

Nature communications

Manieri E,Tie G,Malagola E,Seruggia D,Madha S,Maglieri A,Huang K,Fujiwara Y,Zhang K,Orkin SH,Wang TC,He R,McCarthy N,Shivdasani RA

120-02C was used in Cell Culture to shed light on spatial, molecular, and functional organization of gastric mesenchyme and the spectrum of signaling sources for epithelial support.

Sat Dec 02 00:00:00 EST 2023

Differentiation of Human Induced Pluripotent Stem Cells (iPSCs)-derived Mesenchymal Progenitors into Chondrocytes.

Bio-protocol

Khan NM,Diaz-Hernandez ME,Drissi H

120-02C was used in Cell Culture to describe the detailed procedure for successful derivation of mesenchymal progenitor population from human iPSCs, which are then differentiated into chondrocytes using high-density culture conditions by stimulating with bone morphogenetic protein-2 (BMP-2) or transforming growth factor beta-3 (TGFβ-3).

Sun Nov 05 00:00:00 EDT 2023

Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids.

STAR protocols

Martinez-Silgado A,Yousef Yengej FA,Puschhof J,Geurts V,Boot C,Geurts MH,Rookmaaker MB,Verhaar MC,Beumer J,Clevers H

120-02C was used in In vitro experiments to investigate the effects of BMP agonists on differentiation of human small intestinal organoids.

Fri Sep 16 00:00:00 EDT 2022

Identification of SARS-CoV-2 inhibitors using lung and colonic organoids.

Nature

Han Y,Duan X,Yang L,Nilsson-Payant BE,Wang P,Duan F,Tang X,Yaron TM,Zhang T,Uhl S,Bram Y,Richardson C,Zhu J,Zhao Z,Redmond D,Houghton S,Nguyen DT,Xu D,Wang X,Jessurun J,Borczuk A,Huang Y,Johnson JL,Liu Y,Xiang J,Wang H,Cantley LC,tenOever BR,Ho DD,Pan FC,Evans T,Chen HJ,Schwartz RE,Chen S

Fri Jan 01 00:00:00 EST 2021

Tumor cells generate astrocyte-like cells that contribute to SHH-driven medulloblastoma relapse.

The Journal of experimental medicine

Guo D,Wang Y,Cheng Y,Liao S,Hu J,Du F,Xu G,Liu Y,Cai KQ,Cheung M,Wainwright BJ,Lu QR,Zhao Y,Yang ZJ

Mon Sep 06 00:00:00 EDT 2021

Liver Sinusoidal Endothelial Cells Suppress Bone Morphogenetic Protein 2 Production in Response to TGFβ Pathway Activation.

Hepatology (Baltimore, Md.)

Colucci S,Altamura S,Marques O,Dropmann A,Horvat NK,Müdder K,Hammad S,Dooley S,Muckenthaler MU

Fri Oct 01 00:00:00 EDT 2021

The Effects of Stably Tethered BMP-2 on MC3T3-E1 Preosteoblasts Encapsulated in a PEG Hydrogel.

Biomacromolecules

Schoonraad SA,Trombold ML,Bryant SJ

120-02C was used in In vitro experiments to examine thiolation of bone morphogenetic protein-2 (BMP-2) for chemical attachment to a poly(ethylene glycol) hydrogel using thiol-norbornene click chemistry.

Mon Mar 08 00:00:00 EST 2021

High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells.

Cell

Beumer J,Puschhof J,Bauzá-Martinez J,Martínez-Silgado A,Elmentaite R,James KR,Ross A,Hendriks D,Artegiani B,Busslinger GA,Ponsioen B,Andersson-Rolf A,Saftien A,Boot C,Kretzschmar K,Geurts MH,Bar-Ephraim YE,Pleguezuelos-Manzano C,Post Y,Begthel H,van der Linden F,Lopez-Iglesias C,van de Wetering WJ,van der Linden R,Peters PJ,Heck AJR,Goedhart J,Snippert H,Zilbauer M,Teichmann SA,Wu W,Clevers H

Thu Jun 11 00:00:00 EDT 2020

Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells.

Current protocols in immunology

Pleguezuelos-Manzano C,Puschhof J,van den Brink S,Geurts V,Beumer J,Clevers H

120-02C was used in Cell Culture to provide a basic guide to establishment and maintenance of human intestinal organoids derived from small intestine and colon biopsies and provide an overview of several downstream applications of human intestinal organoids.

Tue Sep 01 00:00:00 EDT 2020

Development of immunocompatible pluripotent stem cells via CRISPR-based human leukocyte antigen engineering.

Experimental & molecular medicine

Jang Y,Choi J,Park N,Kang J,Kim M,Kim Y,Ju JH

Mon Jan 07 00:00:00 EST 2019

Urothelial organoids originating from Cd49f^high mouse stem cells display Notch-dependent differentiation capacity.

Nature communications

Santos CP,Lapi E,Martínez de Villarreal J,Álvaro-Espinosa L,Fernández-Barral A,Barbáchano A,Domínguez O,Laughney AM,Megías D,Muñoz A,Real FX

Fri Sep 27 00:00:00 EDT 2019

Salt-inducible kinase 1 regulates bone anabolism via the CRTC1-CREB-Id1 axis.

Cell death & disease

Kim MK,Kwon JO,Song MK,Kim B,Kim H,Lee ZH,Koo SH,Kim HH

Thu Oct 31 00:00:00 EDT 2019

A comparison of BMP2 delivery by coacervate and gene therapy for promoting human muscle-derived stem cell-mediated articular cartilage repair.

Stem cell research & therapy

Gao X,Cheng H,Awada H,Tang Y,Amra S,Lu A,Sun X,Lv G,Huard C,Wang B,Bi X,Wang Y,Huard J

120-02C was used in In vitro experiments to study the cartilage repair capacity of human muscle-derived stem cells.

Tue Nov 26 00:00:00 EST 2019

Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model.

Scientific reports

Sahakyan V,Duelen R,Tam WL,Roberts SJ,Grosemans H,Berckmans P,Ceccarelli G,Pelizzo G,Broccoli V,Deprest J,Luyten FP,Verfaillie CM,Sampaolesi M

Tue Feb 13 00:00:00 EST 2018

Bioinspired Heparin Nanosponge Prepared by Photo-crosslinking for Controlled Release of Growth Factors.

Scientific reports

Choi WI,Sahu A,Vilos C,Kamaly N,Jo SM,Lee JH,Tae G

Mon Oct 30 00:00:00 EDT 2017

3D Bioprinting Using a Templated Porous Bioink.

Advanced healthcare materials

Armstrong JP,Burke M,Carter BM,Davis SA,Perriman AW

Fri Jul 01 00:00:00 EDT 2016

GDF11 decreases bone mass by stimulating osteoclastogenesis and inhibiting osteoblast differentiation.

Nature communications

Liu W,Zhou L,Zhou C,Zhang S,Jing J,Xie L,Sun N,Duan X,Jing W,Liang X,Zhao H,Ye L,Chen Q,Yuan Q

Thu Sep 22 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 2610024H22Rik; AL117858; AW546137; BB189135; BMP; BMP-2; BMP-2A; BMPR-II; BMPRII; Bone morphogenetic protein; Bone morphogenetic protein 2; Bone morphogenetic protein 2A; H-BMP-2

Human/Mouse/Rat BMP-2 Recombinant Protein, PeproTech®

Human/Mouse/Rat BMP-2 Protein (120-02C-10UG) in Functional

Product Details

120-02C-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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