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Product Details
RP-105433
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DDLQLMKDLGQRVSFLTRDLSSSEPVHAKKRKHERIIDKYEKIPRTLQTAPEKELIHLLPIKDKSGIIPQTREKPV
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (89%), Rat (89%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66939. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
GADD 153, a growth arrest and DNA damage-inducible gene, encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10 or C/EBP zeta). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase during cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus, GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells. GADD 153 belongs to the CBF/MAK21 family, which also includes NOC2L, NOC3L and NOC4L. NOC3L, also designated factor for adipocyte differentiation 24 or Fad24, promotes adipogenesis by controlling DNA replication during the early stages of mitotic clonal expansion (MCE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Factor for adipocyte differentiation 24; NOC3 protein homolog; NOC3-like DNA replication regulator; NOC3-like protein; NOC3L DNA replication regulator; nucleolar complex associated 3 homolog; Nucleolar complex protein 3 homolog; Nucleolar complex-associated protein 3-like protein
Gene Aliases: AD24; C10orf117; FAD24; NOC3L
UniProt ID: (Human) Q8WTT2
Entrez Gene ID: (Human) 64318
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