Gibco

Human NT-4 Recombinant Protein, PeproTech®

Product Details

450-04-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 17 publications 17 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Amphibian, Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA

Molecular weight

28.1 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human NT-4 is a noncovalently linked homodimer of two 14.0 kDa polypeptide monomers (260 total amino acid residues).

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glioma synapses recruit mechanisms of adaptive plasticity.

Nature

Taylor KR,Barron T,Hui A,Spitzer A,Yalçin B,Ivec AE,Geraghty AC,Hartmann GG,Arzt M,Gillespie SM,Kim YS,Maleki Jahan S,Zhang H,Shamardani K,Su M,Ni L,Du PP,Woo PJ,Silva-Torres A,Venkatesh HS,Mancusi R,Ponnuswami A,Mulinyawe S,Keough MB,Chau I,Aziz-Bose R,Tirosh I,Suvà ML,Monje M

450-04 was used in Cell Culture to indicate that brain-derived neurotrophic factor1-3-TrkB signalling promotes malignant synaptic plasticity and augments tumour progression.

Wed Nov 01 00:00:00 EDT 2023

Boosting peripheral BDNF rescues impaired in vivo axonal transport in CMT2D mice.

JCI insight

Sleigh JN,Villarroel-Campos D,Surana S,Wickenden T,Tong Y,Simkin RL,Vargas JNS,Rhymes ER,Tosolini AP,West SJ,Zhang Q,Yang XL,Schiavo G

450-04 was used in In vivo experiments to suggest that selectively targeting muscles with BDNF-boosting therapies could represent a viable therapeutic strategy for CMT2D.

Mon May 08 00:00:00 EDT 2023

Deep learning-based predictive identification of neural stem cell differentiation.

Nature communications

Zhu Y,Huang R,Wu Z,Song S,Cheng L,Zhu R

450-04 was used in Cell Culture to showcase effectiveness of deep learning-based platform in precisely, accurately and robustly detecting NSC differentiation.

Mon May 10 00:00:00 EDT 2021

Deep learning-based predictive identification of neural stem cell differentiation.

Nature communications

Zhu Y,Huang R,Wu Z,Song S,Cheng L,Zhu R

450-04 was used in Cell Culture to showcase effectiveness of deep learning-based platform in precisely, accurately and robustly detecting NSC differentiation.

Mon May 10 00:00:00 EDT 2021

Functionally Distinct Neuronal Ensembles within the Memory Engram.

Cell

Sun X,Bernstein MJ,Meng M,Rao S,Sørensen AT,Yao L,Zhang X,Anikeeva PO,Lin Y

Thu Apr 16 00:00:00 EDT 2020

A TRP channel trio mediates acute noxious heat sensing.

Nature

Vandewauw I,De Clercq K,Mulier M,Held K,Pinto S,Van Ranst N,Segal A,Voet T,Vennekens R,Zimmermann K,Vriens J,Voets T

Thu Mar 29 00:00:00 EDT 2018

VAMP7 regulates constitutive membrane incorporation of the cold-activated channel TRPM8.

Nature communications

Ghosh D,Pinto S,Danglot L,Vandewauw I,Segal A,Van Ranst N,Benoit M,Janssens A,Vennekens R,Vanden Berghe P,Galli T,Vriens J,Voets T

Thu Feb 04 00:00:00 EST 2016

A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR.

Journal of biological methods

Mournetas V,Pereira SM,Fernig DG,Murray P

450-04 was used in Cell Culture to investigate a more efficient and less laborious technique of titrating RNAi lentiviral particles.

Tue Sep 29 00:00:00 EDT 2020

Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line.

Scientific reports

Sluch VM,Davis CH,Ranganathan V,Kerr JM,Krick K,Martin R,Berlinicke CA,Marsh-Armstrong N,Diamond JS,Mao HQ,Zack DJ

Fri Nov 13 00:00:00 EST 2015

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.

The Journal of biological chemistry

Soteriou D,Iskender B,Byron A,Humphries JD,Borg-Bartolo S,Haddock MC,Baxter MA,Knight D,Humphries MJ,Kimber SJ

Fri Jun 28 00:00:00 EDT 2013

Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension.

Nature biotechnology

Steiner D,Khaner H,Cohen M,Even-Ram S,Gil Y,Itsykson P,Turetsky T,Idelson M,Aizenman E,Ram R,Berman-Zaken Y,Reubinoff B

Thu Apr 01 00:00:00 EDT 2010

The Angelman Syndrome protein Ube3A regulates synapse development by ubiquitinating arc.

Cell

Greer PL,Hanayama R,Bloodgood BL,Mardinly AR,Lipton DM,Flavell SW,Kim TK,Griffith EC,Waldon Z,Maehr R,Ploegh HL,Chowdhury S,Worley PF,Steen J,Greenberg ME

Fri Mar 05 00:00:00 EST 2010

Dendritic branching of olfactory bulb mitral and tufted cells: regulation by TrkB.

PloS one

Imamura F,Greer CA

Tue Aug 25 00:00:00 EDT 2009

Fgfr2b mediated epithelial-mesenchymal interactions coordinate tooth morphogenesis and dental trigeminal axon patterning.

Mechanisms of development

Kettunen P,Spencer-Dene B,Furmanek T,Kvinnsland IH,Dickson C,Thesleff I,Luukko K

Wed Mar 19 00:00:00 EDT 2008

The cyclic AMP cascade is altered in the fragile X nervous system.

PloS one

Kelley DJ,Davidson RJ,Elliott JL,Lahvis GP,Yin JC,Bhattacharyya A

Wed Sep 26 00:00:00 EDT 2007

Regulation of apoptosis and differentiation by p53 in human embryonic stem cells.

The Journal of biological chemistry

Qin H,Yu T,Qing T,Liu Y,Zhao Y,Cai J,Li J,Song Z,Qu X,Zhou P,Wu J,Ding M,Deng H

Fri Feb 23 00:00:00 EST 2007

Neurotrophins mediate human embryonic stem cell survival.

Nature biotechnology

Pyle AD,Lock LF,Donovan PJ

Wed Mar 01 00:00:00 EST 2006

Transplantation of expanded mesencephalic precursors leads to recovery in parkinsonian rats.

Nature neuroscience

Studer L,Tabar V,McKay RD

Sat Aug 01 00:00:00 EDT 1998

Neurotrophins induce formation of functional excitatory and inhibitory synapses between cultured hippocampal neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Vicario-Abejón C,Collin C,McKay RD,Segal M

Tue Sep 15 00:00:00 EDT 1998

Cyclic AMP elevation is sufficient to promote the survival of spinal motor neurons in vitro.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Hanson MG,Shen S,Wiemelt AP,McMorris FA,Barres BA

Tue Sep 15 00:00:00 EDT 1998

Regulation of quantal secretion by neurotrophic factors at developing motoneurons in Xenopus cell cultures.

The Journal of physiology

Liou JC,Yang RS,Fu WM

Fri Aug 15 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: neurotrophic factor 4; neurotrophic factor 5; Neurotrophin 4/5; neurotrophin 5 (neurotrophin 4/5); Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT-4; NT-5; NT4/NT5

Human NT-4 Recombinant Protein, PeproTech®

Product Details

450-04-10UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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