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Invitrogen
Human NUAK2 (aa 482-628) Control Fragment Recombinant Protein
Product Details
RP-89800
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (81%), Rat (81%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-82371. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2. NUAK2 is activated by muscle contraction and is a unique mediator of contraction-stimulated glucose transport in skeletal muscle (1). NUAK2 is involved in cellular stress responses linked to obesity and type 2 diabetes. NUAK2 shows kinase activity against a synthetic test peptide and the activity in keratinocytes is increased by AMP and 5-amino-4-imidazolecarboxamide riboside, implying that AMPK kinase-dependent pathway can activate NAUK2. Glucose deprivation also increases NAUK2 activity in baby hamster kidney fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NUAK family SNF1-like kinase 2; NUAK family, SNF1-like kinase, 2; NUAK2; Omphalocele kinase 2; SNARK; SNF1/AMP activated protein kinase; SNF1/AMP kinase-related kinase
Gene Aliases: NUAK2; OMPHK2; SNARK
UniProt ID: (Human) Q9H093
Entrez Gene ID: (Human) 81788
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