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Invitrogen
Human PACS1 (aa 427-524) Control Fragment Recombinant Protein
Product Details
RP-97484
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
DTTSPMELAALEKIKSTWIKNQDDSLTETDTLEITDQDMFGDASTSLVVPEKVKTPMKSSKTDLQGSASPSKVEGVHTPRQKRSTPLKERQLSKPLSE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (96%), Rat (96%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-58589. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
PACS-1 (CRA_a) is a 963 amino acid rich regulatory protein involved in protein trafficking and belongs to the PACS family. A coat protein involved in controlling the correct subcellular localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs, PACS-1 is also known to be involved in HIV-1 Nef-mediated removal of MHC-I from the cell surface to the TGN, thus enabling HIV-1 to escape immune surveillance. PACS-1 also controls the endosome to Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin coated membrane pits. Usually seen in golgi apparatus, trans-Golgi network and localized in the paranuclear region, it is ubiquitously expressed in most of the tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic sorting protein PACS-1; PACS-1; Phosphofurin acidic cluster sorting protein 1
Gene Aliases: KIAA1175; MRD17; PACS1
UniProt ID: (Human) Q6VY07
Entrez Gene ID: (Human) 55690
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