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Product Details
PHG0134
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
Human PDGF-AB recombinant protein is a disulfide-linked heterodimer containing one 125 amino acid alpha chain and one 109 amino acid beta chain
Molecular weight
25.5 kDa
Class
Recombinant
Type
Protein
Purity
≥98% by SDS-PAGE
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 ≤ 1.0 ng/mL; determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 10 mM acetic acid to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-alpha, TGF-beta and EGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: becaplermin; H-PDGF-AB; PDGF A-chain; PDGF subunit A; PDGF subunit B; PDGF, B chain; PDGF-1; PDGF-2; PDGFAB; platelet-derived growth factor 2; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit A; Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis
Gene Aliases: c-sis; IBGC5; PDGF-2; PDGF-A; PDGF1; PDGF2; PDGFA; PDGFB; SIS; SSV
UniProt ID: (Human) P04085, (Human) P01127
Entrez Gene ID: (Human) 5154, (Human) 5155
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