Gibco

Human PDGF-BB, Animal-Free Recombinant Protein, PeproTech®

Human PDGF-BB, Animal-Free Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human PDGF-BB, Animal-Free Protein (AF-100-14B-10UG) in Functional

WHO Comparison of Human PDGF-BB, Animal-Free Recombinant Protein, PeproTech® (Product # AF-100-14B-1MG).

Product Image Gallery

Product Details

AF-100-14B-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 14 publications 14 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human, Mouse, Pig

Expression System

E. coli

Amino acid sequence

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Molecular weight

24.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two beta chains (218 total amino acids).

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cortical bone stem cell-derived exosomes' therapeutic effect on myocardial ischemia-reperfusion and cardiac remodeling.

American journal of physiology. Heart and circulatory physiology

Schena GJ,Murray EK,Hildebrand AN,Headrick AL,Yang Y,Koch KA,Kubo H,Eaton D,Johnson J,Berretta R,Mohsin S,Kishore R,McKinsey TA,Elrod JW,Houser SR

Wed Dec 01 00:00:00 EST 2021

Embelin downregulated cFLIP in breast cancer cell lines facilitate anti-tumor effect of IL-1β-stimulated human umbilical cord mesenchymal stem cells.

Scientific reports

Liang YH,Wu JM,Teng JW,Hung E,Wang HS

Mon Jul 19 00:00:00 EDT 2021

Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG.

Bone research

Chen CY,Rao SS,Tan YJ,Luo MJ,Hu XK,Yin H,Huang J,Hu Y,Luo ZW,Liu ZZ,Wang ZX,Cao J,Liu YW,Li HM,Chen Y,Du W,Liu JH,Zhang Y,Chen TH,Liu HM,Wu B,Yue T,Wang YY,Xia K,Lei PF,Tang SY,Xie H

Wed Sep 30 00:00:00 EDT 2020

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.

Journal of cellular and molecular medicine

Kawamura M,Yamamoto T,Yamashiro K,Kochi S,Yoshihara-Hirata C,Ideguchi H,Aoyagi H,Omori K,Takashiba S

Fri Feb 01 00:00:00 EST 2019

Novel 3-D cell culture system for in vitro evaluation of anticancer drugs under anchorage-independent conditions.

Cancer science

Aihara A,Abe N,Saruhashi K,Kanaki T,Nishino T

Thu Dec 01 00:00:00 EST 2016

Vascular adhesion protein-1 promotes liver inflammation and drives hepatic fibrosis.

The Journal of clinical investigation

Weston CJ,Shepherd EL,Claridge LC,Rantakari P,Curbishley SM,Tomlinson JW,Hubscher SG,Reynolds GM,Aalto K,Anstee QM,Jalkanen S,Salmi M,Smith DJ,Day CP,Adams DH

Sun Feb 01 00:00:00 EST 2015

A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration.

Scientific reports

Wei Y,Chen F,Zhang T,Chen D,Jia X,Wang J,Guo W,Chen J

Mon Sep 14 00:00:00 EDT 2015

A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration.

Scientific reports

Wei Y,Chen F,Zhang T,Chen D,Jia X,Wang J,Guo W,Chen J

Mon Sep 14 00:00:00 EDT 2015

Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis.

Evidence-based complementary and alternative medicine : eCAM

Wang H,Fang Y,Wang Y,Wang Z,Zou Q,Shi Y,Chen J,Peng D

Thu Aug 23 00:00:00 EDT 2012

Systemic perturbation of the ERK signaling pathway by the proteasome inhibitor, MG132.

PloS one

Cirit M,Grant KG,Haugh JM

Tue May 28 00:00:00 EDT 2013

Systemic perturbation of the ERK signaling pathway by the proteasome inhibitor, MG132.

PloS one

Cirit M,Grant KG,Haugh JM

Tue May 28 00:00:00 EDT 2013

CCN2/connective tissue growth factor is essential for pericyte adhesion and endothelial basement membrane formation during angiogenesis.

PloS one

Hall-Glenn F,De Young RA,Huang BL,van Handel B,Hofmann JJ,Chen TT,Choi A,Ong JR,Benya PD,Mikkola H,Iruela-Arispe ML,Lyons KM

Fri Jun 29 00:00:00 EDT 2012

Dasatinib as a bone-modifying agent: anabolic and anti-resorptive effects.

PloS one

Garcia-Gomez A,Ocio EM,Crusoe E,Santamaria C,Hernández-Campo P,Blanco JF,Sanchez-Guijo FM,Hernández-Iglesias T,Briñón JG,Fisac-Herrero RM,Lee FY,Pandiella A,San Miguel JF,Garayoa M

Mon Sep 10 00:00:00 EDT 2012

Decreased expression of vitamin D receptors in neointimal lesions following coronary artery angioplasty in atherosclerotic swine.

PloS one

Gupta GK,Agrawal T,Del Core MG,Hunter WJ,Agrawal DK

Thu Jan 10 00:00:00 EST 2013

Activation of STAT6 by STING is critical for antiviral innate immunity.

Cell

Chen H,Sun H,You F,Sun W,Zhou X,Chen L,Yang J,Wang Y,Tang H,Guan Y,Xia W,Gu J,Ishikawa H,Gutman D,Barber G,Qin Z,Jiang Z

Fri Oct 14 00:00:00 EDT 2011

Biophysical characteristics reveal neural stem cell differentiation potential.

PloS one

Labeed FH,Lu J,Mulhall HJ,Marchenko SA,Hoettges KF,Estrada LC,Lee AP,Hughes MP,Flanagan LA

Thu Jan 26 00:00:00 EST 2012

AP-1 is a component of the transcriptional network regulated by GSK-3 in quiescent cells.

PloS one

Tullai JW,Tacheva S,Owens LJ,Graham JR,Cooper GM

Thu Sep 22 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: becaplermin; FLJ12858; H-PDGF-BB; PDGF subunit B; PDGF, B chain; PDGF-2; platelet-derived growth factor 2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); Platelet-derived growth factor subunit B; platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis

Human PDGF-BB, Animal-Free Recombinant Protein, PeproTech®

Human PDGF-BB, Animal-Free Protein (AF-100-14B-10UG) in Functional

Product Details

AF-100-14B-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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