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Invitrogen
Human PUF60 (aa 328-410) Control Fragment Recombinant Protein
Product Details
RP-104013
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
AATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTL
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (98%), Rat (98%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-84100. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
PUF60 encodes a protein that is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60 kDa poly(U)-binding-splicing factor; FBP interacting repressor; FBP-interacting repressor; FLJ31379; FUSE-binding protein-interacting repressor; poly(U) binding splicing factor 60KDa; Poly(U)-binding-splicing factor PUF60; poly-U binding splicing factor 60KDa; pyrimidine tract binding splicing factor; Ro ribonucleoprotein-binding protein 1; Ro-binding protein 1; RoBP1; Siah binding protein 1; Siah-binding protein 1; Siah-BP1
Gene Aliases: FIR; PUF60; ROBPI; SIAHBP1; VRJS
UniProt ID: (Human) Q9UHX1
Entrez Gene ID: (Human) 22827
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