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Product Details
A42586
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
CHO cells
Amino acid sequence
Human RSPO1, amino acids Met1-Ala263 (Accession # NP_001033722.1)
Molecular weight
26.8 kDa
Class
Recombinant
Type
Protein
Purity
≥95 % as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 10-80 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ40906; hRspo1; R-spondin homolog; R-spondin-1; Roof plate-specific spondin-1; RP11-566C13.1
Gene Aliases: CRISTIN3; RSPO; RSPO1
UniProt ID: (Human) Q2MKA7
Entrez Gene ID: (Human) 284654
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