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Invitrogen
Human SATB2 (aa 377-499) Control Fragment Recombinant Protein
Product Details
RP-93680
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VSQAVFARVAFNRTQGLLSEILRKEEDPRTASQSLLVNLRAMQNFLNLPEVERDRIYQDERERSMNPNVSMVSSASSSPSSSRTPQAKTSTPTTDLPIKVDGANINITAAIYDEIQQEMKRAK
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (99%), Rat (99%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-81914. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
DNA-binding protein SATB2 binds to DNA at the nuclear matrix- or scaffold associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. It also acts as a docking site for several chromatin remodeling enzymes by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. SATB2 is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes - probably by modulating BCL11B expression. It is a repressor of Ctip2 and a regulatory determinant of corticocortical connections in the developing cerebral cortex. Mutations in the gene are associate with cleft palate isolated (CPI).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA-binding protein SATB2; FLJ21474; FLJ32076; MGC119474; MGC119477; SATB family member 2; Special AT-rich sequence-binding protein 2
Gene Aliases: GLSS; KIAA1034; SATB2
UniProt ID: (Human) Q9UPW6
Entrez Gene ID: (Human) 23314
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