Gibco

Human TPO (Thrombopoietin) Recombinant Protein, PeproTech®

Human TPO (Thrombopoietin) Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Human TPO (Thrombopoietin) Protein (300-18-10UG) in Functional

Bioassay analysis of Human TPO (Thrombopoietin) Recombinant Protein, PeproTech® (Product # 300-18-1MG).

Product Image Gallery

Product Details

300-18-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 334 publications 334 publications

Miscellaneous PubMed (Misc)

View 34 publications 34 publications

Product Specifications

Species

Human

Published species

Hamster, Human, Mouse, Non-human primate, Rat, Virus

Expression System

E. coli

Amino acid sequence

SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL

Molecular weight

18.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human TPO is a fully biologically active 174 amino acid polypeptide (18.6 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity.

British journal of haematology

Wang W,Li X,Qin X,Miao Y,Zhang Y,Li S,Yao R,Yang Y,Yu L,Zhu H,Song L,Mao S,Wang X,Chen J,Feng H,Li Y

300-18 was used in In vitro experiments to demonstrate that germline NF1 mutations alone cannot directly drive JMML development and suggest a potential cell immunotherapy for JMML patients.

Sat Jul 01 00:00:00 EDT 2023

FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity.

Cell metabolism

Wieder N,Fried JC,Kim C,Sidhom EH,Brown MR,Marshall JL,Arevalo C,Dvela-Levitt M,Kost-Alimova M,Sieber J,Gabriel KR,Pacheco J,Clish C,Abbasi HS,Singh S,Rutter JC,Therrien M,Yoon H,Lai ZW,Baublis A,Subramanian R,Devkota R,Small J,Sreekanth V,Han M,Lim D,Carpenter AE,Flannick J,Finucane H,Haigis MC,Claussnitzer M,Sheu E,Stevens B,Wagner BK,Choudhary A,Shaw JL,Pablo JL,Greka A

300-18 was used in Cell Culture to find that c-MAF-inducing protein (CMIP) protects cells from FFA exposure by modulating Akt signaling.

Tue May 02 00:00:00 EDT 2023

Long noncoding RNA HIKER regulates erythropoiesis in Monge's disease via CSNK2B.

The Journal of clinical investigation

Azad P,Zhou D,Tu HC,Villafuerte FC,Traver D,Rana TM,Haddad GG

300-18 was used in In vitro experiments to conclude that HIKER regulates erythropoiesis in Monge's disease and acts through at least one specific target, CSNK2B, a casein kinase.

Thu Jun 01 00:00:00 EDT 2023

The tumor suppressor MIR139 is silenced by POLR2M to promote AML oncogenesis.

Leukemia

Stavast CJ,van Zuijen I,Karkoulia E,Özçelik A,van Hoven-Beijen A,Leon LG,Voerman JSA,Janssen GMC,van Veelen PA,Burocziova M,Brouwer RWW,van IJcken WFJ,Maas A,Bindels EM,van der Velden VHJ,Schliehe C,Katsikis PD,Alberich-Jorda M,Erkeland SJ

300-18 was used in Cell Culture to investigate miRNA-139 in acute myeloid leukemia.

Tue Mar 01 00:00:00 EST 2022

A comparison of DNA repair pathways to achieve a site-specific gene modification of the Bruton's tyrosine kinase gene.

Molecular therapy. Nucleic acids

Gray DH,Santos J,Keir AG,Villegas I,Maddock S,Trope EC,Long JD,Kuo CY

300-18 was used in Cell Culture to compare three methods of targeted insertion — homology-directed repair (HDR), homology-independent targeted integration (HITI), and precise integration into target chromosome (PITCh) — to achieve a permanent gene-therapy-based cure for X-linked agammaglobulinemia.

Tue Mar 08 00:00:00 EST 2022

Using Cryo-ET to distinguish platelets during pre-acute myeloid leukemia from steady state hematopoiesis.

Communications biology

Wang Y,Huo T,Tseng YJ,Dang L,Yu Z,Yu W,Foulks Z,Murdaugh RL,Ludtke SJ,Nakada D,Wang Z

Thu Jan 20 00:00:00 EST 2022

Single-chain tandem macrocyclic peptides as a scaffold for growth factor and cytokine mimetics.

Communications biology

Ito K,Matsuda Y,Mine A,Shikida N,Takahashi K,Miyairi K,Shimbo K,Kikuchi Y,Konishi A

Fri Jan 14 00:00:00 EST 2022

Generation, expansion, and drug treatment of hematopoietic progenitor cells derived from human iPSCs.

STAR protocols

Dannenmann B,Skokowa J

300-18 was used in Cell Culture to provide protocol for generation of HPCs from iPSCs followed by HPC expansion on Sl/Sl feeder cells expressing FLT3L.

Fri Jun 17 00:00:00 EDT 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

300-18 was used in Cell Culture to demonstrate that interferon-mediated cell cycle control underlies the clinical efficacy of glucocorticoids.

Tue May 10 00:00:00 EDT 2022

ZNF410 represses fetal globin by singular control of CHD4.

Nature genetics

Vinjamur DS,Yao Q,Cole MA,McGuckin C,Ren C,Zeng J,Hossain M,Luk K,Wolfe SA,Pinello L,Bauer DE

300-18 was used in Cell Culture to identify zinc-finger protein (ZNF) 410 as an fetal hemoglobin repressor.

Sat May 01 00:00:00 EDT 2021

N⁶-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation.

Cancer cell

Cheng Y,Xie W,Pickering BF,Chu KL,Savino AM,Yang X,Luo H,Nguyen DT,Mo S,Barin E,Velleca A,Rohwetter TM,Patel DJ,Jaffrey SR,Kharas MG

300-18 was used in In vitro experiments to investigate How N6-Methyladenosine dictates its diverse molecular and cellular effects in leukemias.

Mon Jul 12 00:00:00 EDT 2021

Enhancer Hijacking Drives Oncogenic BCL11B Expression in Lineage-Ambiguous Stem Cell Leukemia.

Cancer discovery

Montefiori LE,Bendig S,Gu Z,Chen X,Pölönen P,Ma X,Murison A,Zeng A,Garcia-Prat L,Dickerson K,Iacobucci I,Abdelhamed S,Hiltenbrand R,Mead PE,Mehr CM,Xu B,Cheng Z,Chang TC,Westover T,Ma J,Stengel A,Kimura S,Qu C,Valentine MB,Rashkovan M,Luger S,Litzow MR,Rowe JM,den Boer ML,Wang V,Yin J,Kornblau SM,Hunger SP,Loh ML,Pui CH,Yang W,Crews KR,Roberts KG,Yang JJ,Relling MV,Evans WE,Stock W,Paietta EM,Ferrando AA,Zhang J,Kern W,Haferlach T,Wu G,Dick JE,Klco JM,Haferlach C,Mullighan CG

300-18 was used in In vitro experiments to identify oncogenic deregulation of BCL11B driven by diverse structural alterations, including de novo superenhancer generation, as the driving feature of a subset of lineage-ambiguous leukemias that transcend current diagnostic boundaries.

Mon Nov 01 00:00:00 EDT 2021

M2 macrophages, but not M1 macrophages, support megakaryopoiesis by upregulating PI3K-AKT pathway activity.

Signal transduction and targeted therapy

Zhao HY,Zhang YY,Xing T,Tang SQ,Wen Q,Lyu ZS,Lv M,Wang Y,Xu LP,Zhang XH,Kong Y,Huang XJ

Fri Jun 18 00:00:00 EDT 2021

Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and aging.

Cell stem cell

Kruta M,Sunshine MJ,Chua BA,Fu Y,Chawla A,Dillingham CH,Hidalgo San Jose L,De Jong B,Zhou FJ,Signer RAJ

Thu Nov 04 00:00:00 EDT 2021

MYC Promotes Bone Marrow Stem Cell Dysfunction in Fanconi Anemia.

Cell stem cell

Rodríguez A,Zhang K,Färkkilä A,Filiatrault J,Yang C,Velázquez M,Furutani E,Goldman DC,García de Teresa B,Garza-Mayén G,McQueen K,Sambel LA,Molina B,Torres L,González M,Vadillo E,Pelayo R,Fleming WH,Grompe M,Shimamura A,Hautaniemi S,Greenberger J,Frías S,Parmar K,D'Andrea AD

300-18 was used in In vitro experiments to identify determinants of bone marrow failure, we performed single-cell transcriptome profiling of primary hematopoietic stem and progenitor cells from Fanconi anemia patients.

Thu Jan 07 00:00:00 EST 2021

HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.

Cell stem cell

Lv K,Gong C,Antony C,Han X,Ren JG,Donaghy R,Cheng Y,Pellegrino S,Warren AJ,Paralkar VR,Tong W

300-18 was used in In vitro experiments to reveal a role of the E3 ubiquitin ligase HectD1 in regulating HSC function via ribosome assembly and protein translation.

Thu Jul 01 00:00:00 EDT 2021

Reconstructing the Lineage Histories and Differentiation Trajectories of Individual Cancer Cells in Myeloproliferative Neoplasms.

Cell stem cell

Van Egeren D,Escabi J,Nguyen M,Liu S,Reilly CR,Patel S,Kamaz B,Kalyva M,DeAngelo DJ,Galinsky I,Wadleigh M,Winer ES,Luskin MR,Stone RM,Garcia JS,Hobbs GS,Camargo FD,Michor F,Mullally A,Cortes-Ciriano I,Hormoz S

Thu Mar 04 00:00:00 EST 2021

A DL-4- and TNFα-based culture system to generate high numbers of nonmodified or genetically modified immunotherapeutic human T-lymphoid progenitors.

Cellular & molecular immunology

Moirangthem RD,Ma K,Lizot S,Cordesse A,Olivré J,de Chappedelaine C,Joshi A,Cieslak A,Tchen J,Cagnard N,Asnafi V,Rausell A,Simons L,Zuber J,Taghon T,Staal FJT,Pflumio F,Six E,Cavazzana M,Lagresle-Peyrou C,Soheili T,André I

Thu Jul 01 00:00:00 EDT 2021

Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes.

The Journal of clinical investigation

Nagamachi A,Kanai A,Nakamura M,Okuda H,Yokoyama A,Shinriki S,Matsui H,Inaba T

300-18 was used in Cell Culture to conclude that Samd9/9L is a downstream regulator of IFN that controls receptor metabolism, with constitutive activation leading to multiorgan dysfunction.

Mon Feb 15 00:00:00 EST 2021

Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes.

The Journal of clinical investigation

Nagamachi A,Kanai A,Nakamura M,Okuda H,Yokoyama A,Shinriki S,Matsui H,Inaba T

300-18 was used in Cell Culture to conclude that Samd9/9L is a downstream regulator of IFN that controls receptor metabolism, with constitutive activation leading to multiorgan dysfunction.

Mon Feb 15 00:00:00 EST 2021

Plasma from patients with bacterial sepsis or severe COVID-19 induces suppressive myeloid cell production from hematopoietic progenitors in vitro.

Science translational medicine

Reyes M,Filbin MR,Bhattacharyya RP,Sonny A,Mehta A,Billman K,Kays KR,Pinilla-Vera M,Benson ME,Cosimi LA,Hung DT,Levy BD,Villani AC,Sade-Feldman M,Baron RM,Goldberg MB,Blainey PC,Hacohen N

Wed Jun 16 00:00:00 EDT 2021

JAK2V617F myeloproliferative neoplasm eradication by a novel interferon/arsenic therapy involves PML.

The Journal of experimental medicine

Dagher T,Maslah N,Edmond V,Cassinat B,Vainchenker W,Giraudier S,Pasquier F,Verger E,Niwa-Kawakita M,Lallemand-Breitenbach V,Plo I,Kiladjian JJ,Villeval JL,de Thé H

Mon Feb 01 00:00:00 EST 2021

Familial thrombocytopenia due to a complex structural variant resulting in a WAC-ANKRD26 fusion transcript.

The Journal of experimental medicine

Wahlster L,Verboon JM,Ludwig LS,Black SC,Luo W,Garg K,Voit RA,Collins RL,Garimella K,Costello M,Chao KR,Goodrich JK,DiTroia SP,O'Donnell-Luria A,Talkowski ME,Michelson AD,Cantor AB,Sankaran VG

Mon Jun 07 00:00:00 EDT 2021

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Mon Feb 01 00:00:00 EST 2021

Constrained chromatin accessibility in PU.1-mutated agammaglobulinemia patients.

The Journal of experimental medicine

Le Coz C,Nguyen DN,Su C,Nolan BE,Albrecht AV,Xhani S,Sun D,Demaree B,Pillarisetti P,Khanna C,Wright F,Chen PA,Yoon S,Stiegler AL,Maurer K,Garifallou JP,Rymaszewski A,Kroft SH,Olson TS,Seif AE,Wertheim G,Grant SFA,Vo LT,Puck JM,Sullivan KE,Routes JM,Zakharova V,Shcherbina A,Mukhina A,Rudy NL,Hurst ACE,Atkinson TP,Boggon TJ,Hakonarson H,Abate AR,Hajjar J,Nicholas SK,Lupski JR,Verbsky J,Chinn IK,Gonzalez MV,Wells AD,Marson A,Poon GMK,Romberg N

Mon Jul 05 00:00:00 EDT 2021

Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome.

EMBO molecular medicine

Vavassori V,Mercuri E,Marcovecchio GE,Castiello MC,Schiroli G,Albano L,Margulies C,Buquicchio F,Fontana E,Beretta S,Merelli I,Cappelleri A,Rancoita PM,Lougaris V,Plebani A,Kanariou M,Lankester A,Ferrua F,Scanziani E,Cotta-Ramusino C,Villa A,Naldini L,Genovese P

Fri Mar 05 00:00:00 EST 2021

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Developmental cell

Ma Y,Liu S,Gao J,Chen C,Zhang X,Yuan H,Chen Z,Yin X,Sun C,Mao Y,Zhou F,Shao Y,Liu Q,Xu J,Cheng L,Yu D,Li P,Yi P,He J,Geng G,Guo Q,Si Y,Zhao H,Li H,Banes GL,Liu H,Nakamura Y,Kurita R,Huang Y,Wang X,Wang F,Fang G,Engel JD,Shi L,Zhang YE,Yu J

300-18 was used in Sample Preparation (PCR) to investigate pseudogene phenotypic effects.

Mon Feb 22 00:00:00 EST 2021

Pediatric MDS and bone marrow failure-associated germline mutations in SAMD9 and SAMD9L impair multiple pathways in primary hematopoietic cells.

Leukemia

Thomas ME,Abdelhamed S,Hiltenbrand R,Schwartz JR,Sakurada SM,Walsh M,Song G,Ma J,Pruett-Miller SM,Klco JM

Mon Nov 01 00:00:00 EDT 2021

Reducing TGF-β1 cooperated with StemRegenin 1 promoted the expansion ex vivo of cord blood CD34⁺ cells by inhibiting AhR signalling.

Cell proliferation

Zhu X,Sun Q,Tan WS,Cai H

Mon Mar 01 00:00:00 EST 2021

Ex vivo conditioning of peripheral blood mononuclear cells of diabetic patients promotes vasculogenic wound healing.

Stem cells translational medicine

Tanaka R,Ito-Hirano R,Fujimura S,Arita K,Hagiwara H,Mita T,Itoh M,Kawaji H,Ogawa T,Watada H,Masuda H,Asahara T,Mizuno H

Tue Jun 01 00:00:00 EDT 2021

Enhanced self-renewal of human long-term hematopoietic stem cells by a sulfamoyl benzoate derivative targeting p18INK4C.

Blood advances

Li Y,Zhang W,Zhang Y,Ding Y,Yang M,He M,Liu X,Gu J,Xu S,Feng Z,Li Y,Yin J,Gao H,Song H,Xu H,Wang C,Ji Q,Ma S,Yang W,Yuan W,Xie XQ,Cheng T,Gao Y

Tue Sep 14 00:00:00 EDT 2021

Zinc-dependent multimerization of mutant calreticulin is required for MPL binding and MPN pathogenesis.

Blood advances

Rivera JF,Baral AJ,Nadat F,Boyd G,Smyth R,Patel H,Burman EL,Alameer G,Boxall SA,Jackson BR,Baxter EJ,Laslo P,Green AR,Kent DG,Mullally A,Chen E

Tue Apr 13 00:00:00 EDT 2021

Engraftment characterization of risk-stratified AML in NSGS mice.

Blood advances

Díaz de la Guardia R,Velasco-Hernandez T,Gutiérrez-Agüera F,Roca-Ho H,Molina O,Nombela-Arrieta C,Bataller A,Fuster JL,Anguita E,Vives S,Zamora L,Nomdedeu J,Gómez-Casares MT,Ramírez-Orellana M,Lapillonne H,Ramos-Mejia V,Rodríguez-Manzaneque JC,Bueno C,Lopez-Millan B,Menéndez P

Tue Dec 14 00:00:00 EST 2021

CRISPR-edited megakaryocytes for rapid screening of platelet gene functions.

Blood advances

Montenont E,Bhatlekar S,Jacob S,Kosaka Y,Manne BK,Lee O,Parra-Izquierdo I,Tugolukova E,Tolley ND,Rondina MT,Bray PF,Rowley JW

Tue May 11 00:00:00 EDT 2021

Lentiviral correction of enzymatic activity restrains macrophage inflammation in adenosine deaminase 2 deficiency.

Blood advances

Zoccolillo M,Brigida I,Barzaghi F,Scala S,Hernández RJ,Basso-Ricci L,Colantuoni M,Pettinato E,Sergi LS,Milardi G,Capasso P,Lombardo A,Gregori S,Sanvito F,Schena F,Cesaro S,Conti F,Pession A,Benedetti F,Gattorno M,Lee PY,Naldini L,Cicalese MP,Aiuti A,Mortellaro A

Tue Aug 24 00:00:00 EDT 2021

CRISPR editing of the GLI1 first intron abrogates GLI1 expression and differentially alters lineage commitment.

Stem cells (Dayton, Ohio)

Galat Y,Gu H,Perepitchka M,Taylor R,Yoon JW,Glukhova XA,Li XN,Beletsky IP,Walterhouse DO,Galat V,Iannaccone PM

Sat May 01 00:00:00 EDT 2021

CRISPR editing of the GLI1 first intron abrogates GLI1 expression and differentially alters lineage commitment.

Stem cells (Dayton, Ohio)

Galat Y,Gu H,Perepitchka M,Taylor R,Yoon JW,Glukhova XA,Li XN,Beletsky IP,Walterhouse DO,Galat V,Iannaccone PM

Sat May 01 00:00:00 EDT 2021

HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant Dendritic Cells.

Stem cells international

Kwon YW,Ahn HS,Lee JW,Yang HM,Cho HJ,Kim SJ,Lee SH,Yang HM,Jang HD,Kim SJ,Kim HS

Tue Jun 08 00:00:00 EDT 2021

Distinct genetic pathways define pre-malignant versus compensatory clonal hematopoiesis in Shwachman-Diamond syndrome.

Nature communications

Kennedy AL,Myers KC,Bowman J,Gibson CJ,Camarda ND,Furutani E,Muscato GM,Klein RH,Ballotti K,Liu S,Harris CE,Galvin A,Malsch M,Dale D,Gansner JM,Nakano TA,Bertuch A,Vlachos A,Lipton JM,Castillo P,Connelly J,Churpek J,Edwards JR,Hijiya N,Ho RH,Hofmann I,Huang JN,Keel S,Lamble A,Lau BW,Norkin M,Stieglitz E,Stock W,Walkovich K,Boettcher S,Brendel C,Fleming MD,Davies SM,Weller EA,Bahl C,Carter SL,Shimamura A,Lindsley RC

Fri Feb 26 00:00:00 EST 2021

Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia.

Cell reports

Simeoni F,Romero-Camarero I,Camera F,Amaral FMR,Sinclair OJ,Papachristou EK,Spencer GJ,Lie-A-Ling M,Lacaud G,Wiseman DH,Carroll JS,Somervaille TCP

Tue Sep 21 00:00:00 EDT 2021

Stem-cell-derived human microglia transplanted into mouse brain to study human disease.

Nature protocols

Fattorelli N,Martinez-Muriana A,Wolfs L,Geric I,De Strooper B,Mancuso R

300-18 was used in In vitro experiments to explain the step-by-step workflow that includes in vitro microglia differentiation from human PSCs, transplantation into the mouse brain and quantitative analysis of engraftment. This approach is more optimized and more efficient approach that yields up to 80% chimerism when compared to current differentiation and xenotransplantation protocols.

Mon Feb 01 00:00:00 EST 2021

Impact of the rs1024611 Polymorphism of CCL2 on the Pathophysiology and Outcome of Primary Myelofibrosis.

Cancers

Masselli E,Carubbi C,Pozzi G,Percesepe A,Campanelli R,Villani L,Gobbi G,Bonomini S,Roti G,Rosti V,Massa M,Barosi G,Vitale M

300-18 was used in In vitro experiments to conclude that the identification of the role of the CCL2/CCR2 chemokine system in PMF adds a novel element to the pathophysiological picture of the disease, with clinical and therapeutic implications.

Sat May 22 00:00:00 EDT 2021

Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice.

Communications biology

Barcia Durán JG,Lu T,Houghton S,Geng F,Schreiner R,Xiang J,Rafii S,Redmond D,Lis R

Thu Mar 25 00:00:00 EDT 2021

Oncogene-induced senescence in hematopoietic progenitors features myeloid restricted hematopoiesis, chronic inflammation and histiocytosis.

Nature communications

Biavasco R,Lettera E,Giannetti K,Gilioli D,Beretta S,Conti A,Scala S,Cesana D,Gallina P,Norelli M,Basso-Ricci L,Bondanza A,Cavalli G,Ponzoni M,Dagna L,Doglioni C,Aiuti A,Merelli I,Di Micco R,Montini E

Tue Jul 27 00:00:00 EDT 2021

The miR-424(322)/503 gene cluster regulates pro- versus anti-inflammatory skin DC subset differentiation by modulating TGF-β signaling.

Cell reports

Zyulina V,Yan KK,Ju B,Schwarzenberger E,Passegger C,Tam-Amersdorfer C,Pan Q,Sconocchia T,Pollack C,Shaner B,Zebisch A,Easton J,Yu J,Silva JM,Strobl H

Tue Apr 27 00:00:00 EDT 2021

Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo.

Cell reports

Dignum T,Varnum-Finney B,Srivatsan SR,Dozono S,Waltner O,Heck AM,Ishida T,Nourigat-McKay C,Jackson DL,Rafii S,Trapnell C,Bernstein ID,Hadland B

300-18 was used in In vitro experiments to suggest a revised model of developmental hematopoiesis in which the initial populations of multipotent progenitors and HSCs arise independently from HE with distinct phenotypic and transcriptional properties.

Tue Sep 14 00:00:00 EDT 2021

Combined epigenetic and metabolic treatments overcome differentiation blockade in acute myeloid leukemia.

iScience

Zee BM,Poels KE,Yao CH,Kawabata KC,Wu G,Duy C,Jacobus WD,Senior E,Endress JE,Jambhekar A,Lovitch SB,Ma J,Dhall A,Harris IS,Blanco MA,Sykes DB,Licht JD,Weinstock DM,Melnick A,Haigis MC,Michor F,Shi Y

Fri Jun 25 00:00:00 EDT 2021

Correction of pathology in mice displaying Gaucher disease type 1 by a clinically-applicable lentiviral vector.

Molecular therapy. Methods & clinical development

Dahl M,Smith EMK,Warsi S,Rothe M,Ferraz MJ,Aerts JMFG,Golipour A,Harper C,Pfeifer R,Pizzurro D,Schambach A,Mason C,Karlsson S

Fri Mar 12 00:00:00 EST 2021

Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays.

STAR protocols

Rodríguez A,Filiatrault J,Flores-Guzmán P,Mayani H,Parmar K,D'Andrea AD

300-18 was used in Cell Culture to present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques.

Fri Dec 17 00:00:00 EST 2021

Isolation of human and murine hematopoietic stem cells for DNA damage and DNA repair assays.

STAR protocols

Rodríguez A,Filiatrault J,Flores-Guzmán P,Mayani H,Parmar K,D'Andrea AD

300-18 was used in Cell Culture to present protocols for the isolation of human and murine HSPCs using manual and FACS-assisted techniques.

Fri Dec 17 00:00:00 EST 2021

Directly reprogrammed natural killer cells for cancer immunotherapy.

Nature biomedical engineering

Kim HS,Kim JY,Seol B,Song CL,Jeong JE,Cho YS

Mon Nov 01 00:00:00 EDT 2021

The therapeutic potential of multiclonal tumoricidal T cells derived from tumor infiltrating lymphocyte-1derived iPS cells.

Communications biology

Ito T,Kawai Y,Yasui Y,Iriguchi S,Minagawa A,Ishii T,Miyoshi H,Taketo MM,Kawada K,Obama K,Sakai Y,Kaneko S

Mon Jun 07 00:00:00 EDT 2021

NOTCH-mediated ex vivo expansion of human hematopoietic stem and progenitor cells by culture under hypoxia.

Stem cell reports

Araki D,Fu JF,Huntsman H,Cordes S,Seifuddin F,Alvarado LJ,Cheruku PS,Cash A,Traba J,Li Y,Pirooznia M,Smith RH,Larochelle A

Tue Sep 14 00:00:00 EDT 2021

Molecular characterization of hematopoietic stem cells after in vitro amplification on biomimetic 3D PDMS cell culture scaffolds.

Scientific reports

Marx-Blümel L,Marx C,Sonnemann J,Weise F,Hampl J,Frey J,Rothenburger L,Cirri E,Rahnis N,Koch P,Groth M,Schober A,Wang ZQ,Beck JF

Wed Oct 27 00:00:00 EDT 2021

Methylation of dual-specificity phosphatase 4 controls cell differentiation.

Cell reports

Su H,Jiang M,Senevirathne C,Aluri S,Zhang T,Guo H,Xavier-Ferrucio J,Jin S,Tran NT,Liu SM,Sun CW,Zhu Y,Zhao Q,Chen Y,Cable L,Shen Y,Liu J,Qu CK,Han X,Klug CA,Bhatia R,Chen Y,Nimer SD,Zheng YG,Iancu-Rubin C,Jin J,Deng H,Krause DS,Xiang J,Verma A,Luo M,Zhao X

Tue Jul 27 00:00:00 EDT 2021

Iron control of erythroid microtubule cytoskeleton as a potential target in treatment of iron-restricted anemia.

Nature communications

Goldfarb AN,Freeman KC,Sahu RK,Elagib KE,Holy M,Arneja A,Polanowska-Grabowska R,Gru AA,White Z,Khalil S,Kerins MJ,Ooi A,Leitinger N,Luckey CJ,Delehanty LL

Fri Mar 12 00:00:00 EST 2021

CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells.

Nature communications

Boutin J,Rosier J,Cappellen D,Prat F,Toutain J,Pennamen P,Bouron J,Rooryck C,Merlio JP,Lamrissi-Garcia I,Cullot G,Amintas S,Guyonnet-Duperat V,Ged C,Blouin JM,Richard E,Dabernat S,Moreau-Gaudry F,Bedel A

Fri Aug 13 00:00:00 EDT 2021

SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis.

Cell reports

Jung HS,Uenishi G,Park MA,Liu P,Suknuntha K,Raymond M,Choi YJ,Thomson JA,Ong IM,Slukvin II

300-18 was used in In vitro experiments to investigate the role and mechanisms of SOX17 in human pluripotent stem cells.

Tue Feb 16 00:00:00 EST 2021

CRISPECTOR provides accurate estimation of genome editing translocation and off-target activity from comparative NGS data.

Nature communications

Amit I,Iancu O,Levy-Jurgenson A,Kurgan G,McNeill MS,Rettig GR,Allen D,Breier D,Ben Haim N,Wang Y,Anavy L,Hendel A,Yakhini Z

Mon May 24 00:00:00 EDT 2021

Development of allogeneic HSC-engineered iNKT cells for off-the-shelf cancer immunotherapy.

Cell reports. Medicine

Li YR,Zhou Y,Kim YJ,Zhu Y,Ma F,Yu J,Wang YC,Chen X,Li Z,Zeng S,Wang X,Lee D,Ku J,Tsao T,Hardoy C,Huang J,Cheng D,Montel-Hagen A,Seet CS,Crooks GM,Larson SM,Sasine JP,Wang X,Pellegrini M,Ribas A,Kohn DB,Witte O,Wang P,Yang L

300-18 was used in In vitro experiments to investigate cancer treatment by cell-based immunotherapy.

Tue Nov 16 00:00:00 EST 2021

The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells.

Nature communications

Sharma R,Dever DP,Lee CM,Azizi A,Pan Y,Camarena J,Köhnke T,Bao G,Porteus MH,Majeti R

Wed Jan 20 00:00:00 EST 2021

Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation.

Nature communications

Georgolopoulos G,Psatha N,Iwata M,Nishida A,Som T,Yiangou M,Stamatoyannopoulos JA,Vierstra J

Tue Nov 23 00:00:00 EST 2021

Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics.

Nature communications

Velten L,Story BA,Hernández-Malmierca P,Raffel S,Leonce DR,Milbank J,Paulsen M,Demir A,Szu-Tu C,Frömel R,Lutz C,Nowak D,Jann JC,Pabst C,Boch T,Hofmann WK,Müller-Tidow C,Trumpp A,Haas S,Steinmetz LM

Mon Mar 01 00:00:00 EST 2021

Novel culture media enhances mononuclear cells from patients with chronic limb-threatening ischemia to increase vasculogenesis and anti-inflammatory effect.

Stem cell research & therapy

Chruewkamlow N,Pruekprasert K,Phutthakunphithak P,Acharayothin O,Prapassaro T,Hongku K,Hahtapornsawan S,Puangpunngam N,Chinsakchai K,Wongwanit C,Ruangsetakit C,Sermsathanasawadi N

300-18 was used in Cell Culture to evaluate the use of Quality and Quantity culture media to culture mononuclear cells from chronic limb-threatening ischemia patients.

Tue Sep 28 00:00:00 EDT 2021

A human fetal liver-derived infant MLL-AF4 acute lymphoblastic leukemia model reveals a distinct fetal gene expression program.

Nature communications

Rice S,Jackson T,Crump NT,Fordham N,Elliott N,O'Byrne S,Fanego MDML,Addy D,Crabb T,Dryden C,Inglott S,Ladon D,Wright G,Bartram J,Ancliff P,Mead AJ,Halsey C,Roberts I,Milne TA,Roy A

Thu Nov 25 00:00:00 EST 2021

Combination of tyrosine kinase inhibitors and the MCL1 inhibitor S63845 exerts synergistic antitumorigenic effects on CML cells.

Cell death & disease

Malyukova A,Ujvari D,Yektaei-Karin E,Zovko A,Madapura HS,Keszei M,Nagy N,Lotfi K,Björn N,Wallvik J,Tamai M,Nguyen TTT,Akahane K,Inukai T,Stenke L,Salamon D

Sat Sep 25 00:00:00 EDT 2021

Effective, safe, and sustained correction of murine XLA using a UCOE-BTK promoter-based lentiviral vector.

Molecular therapy. Methods & clinical development

Seymour BJ,Singh S,Certo HM,Sommer K,Sather BD,Khim S,Clough C,Hale M,Pangallo J,Ryu BY,Khan IF,Adair JE,Rawlings DJ

Fri Mar 12 00:00:00 EST 2021

Long non-coding RNAs as novel therapeutic targets in juvenile myelomonocytic leukemia.

Scientific reports

Hofmans M,Lammens T,Depreter B,Wu Y,Erlacher M,Caye A,Cavé H,Flotho C,de Haas V,Niemeyer CM,Stary J,Van Nieuwerburgh F,Deforce D,Van Loocke W,Van Vlierberghe P,Philippé J,De Moerloose B

Tue Feb 02 00:00:00 EST 2021

Hematopoietic Stem Cell Heterogeneity Is Linked to the Initiation and Therapeutic Response of Myeloproliferative Neoplasms.

Cell stem cell

Tong J,Sun T,Ma S,Zhao Y,Ju M,Gao Y,Zhu P,Tan P,Fu R,Zhang A,Wang D,Wang D,Xiao Z,Zhou J,Yang R,Loughran SJ,Li J,Green AR,Bresnick EH,Wang D,Cheng T,Zhang L,Shi L

Thu Mar 04 00:00:00 EST 2021

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis.

Nature

Nikolaev M,Mitrofanova O,Broguiere N,Geraldo S,Dutta D,Tabata Y,Elci B,Brandenberg N,Kolotuev I,Gjorevski N,Clevers H,Lutolf MP

Tue Sep 01 00:00:00 EDT 2020

Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion.

Cancer cell

Su R,Dong L,Li Y,Gao M,Han L,Wunderlich M,Deng X,Li H,Huang Y,Gao L,Li C,Zhao Z,Robinson S,Tan B,Qing Y,Qin X,Prince E,Xie J,Qin H,Li W,Shen C,Sun J,Kulkarni P,Weng H,Huang H,Chen Z,Zhang B,Wu X,Olsen MJ,Müschen M,Marcucci G,Salgia R,Li L,Fathi AT,Li Z,Mulloy JC,Wei M,Horne D,Chen J

Mon Jul 13 00:00:00 EDT 2020

Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion.

Cancer cell

Mon Jul 13 00:00:00 EDT 2020

The Folate Cycle Enzyme MTHFR Is a Critical Regulator of Cell Response to MYC-Targeting Therapies.

Cancer discovery

Su A,Ling F,Vaganay C,Sodaro G,Benaksas C,Dal Bello R,Forget A,Pardieu B,Lin KH,Rutter JC,Bassil CF,Fortin G,Pasanisi J,Antony-Debré I,Alexe G,Benoist JF,Pruvost A,Pikman Y,Qi J,Schlageter MH,Micol JB,Roti G,Cluzeau T,Dombret H,Preudhomme C,Fenouille N,Benajiba L,Golan HM,Stegmaier K,Lobry C,Wood KC,Itzykson R,Puissant A

300-18 was used in Cell Culture to investigate the effects of MTHFR for its therapeutic effects on acute myeloid leukemia.

Tue Dec 01 00:00:00 EST 2020

Salt-inducible kinase inhibition suppresses acute myeloid leukemia progression in vivo.

Blood

Tarumoto Y,Lin S,Wang J,Milazzo JP,Xu Y,Lu B,Yang Z,Wei Y,Polyanskaya S,Wunderlich M,Gray NS,Stegmaier K,Vakoc CR

Thu Jan 02 00:00:00 EST 2020

Lysine-specific demethylase 1A restricts ex vivo propagation of human HSCs and is a target of UM171.

Blood

Subramaniam A,Žemaitis K,Talkhoncheh MS,Yudovich D,Bäckström A,Debnath S,Chen J,Jain MV,Galeev R,Gaetani M,Zubarev RA,Larsson J

Thu Nov 05 00:00:00 EST 2020

Activation of the receptor tyrosine kinase RET improves long-term hematopoietic stem cell outgrowth and potency.

Blood

Grey W,Chauhan R,Piganeau M,Huerga Encabo H,Garcia-Albornoz M,McDonald NQ,Bonnet D

Thu Nov 26 00:00:00 EST 2020

Bone marrow-derived myeloid progenitors as driver mutation carriers in high- and low-risk Langerhans cell histiocytosis.

Blood

Xiao Y,van Halteren AGS,Lei X,Borst J,Steenwijk E,de Wit T,Grabowska J,Voogd R,Kemps P,Picarsic J,van den Bos C,Borst J

Thu Nov 05 00:00:00 EST 2020

RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia.

Cell stem cell

Shen C,Sheng Y,Zhu AC,Robinson S,Jiang X,Dong L,Chen H,Su R,Yin Z,Li W,Deng X,Chen Y,Hu YC,Weng H,Huang H,Prince E,Cogle CR,Sun M,Zhang B,Chen CW,Marcucci G,He C,Qian Z,Chen J

300-18 was used in Cell Culture to reveal crucial functions of ALKBH5 in leukemogenesis and LSC/LIC self-renewal/maintenance and highlight the therapeutic potential of targeting the ALKBH5/m6A axis.

Thu Jul 02 00:00:00 EDT 2020

Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development.

Cell stem cell

Crosse EI,Gordon-Keylock S,Rybtsov S,Binagui-Casas A,Felchle H,Nnadi NC,Kirschner K,Chandra T,Tamagno S,Webb DJ,Rossi F,Anderson RA,Medvinsky A

300-18 was used in In vitro experiments to identify secreted signals underlying human HSC development, combining spatial transcriptomics analysis of dorsoventral polarized signaling in the aorta with gene expression profiling of sorted cell populations and single cells.

Thu Nov 05 00:00:00 EST 2020

Small-Molecule PAPD5 Inhibitors Restore Telomerase Activity in Patient Stem Cells.

Cell stem cell

Nagpal N,Wang J,Zeng J,Lo E,Moon DH,Luk K,Braun RO,Burroughs LM,Keel SB,Reilly C,Lindsley RC,Wolfe SA,Tai AK,Cahan P,Bauer DE,Fong YW,Agarwal S

300-18 was used in Cell Culture to describe small-molecule PAPD5 inhibitors that demonstrate telomere restoration in vitro, in stem cell models, and in vivo.

Thu Jun 04 00:00:00 EDT 2020

Mesenchymal niche remodeling impairs hematopoiesis via stanniocalcin 1 in acute myeloid leukemia.

The Journal of clinical investigation

Waclawiczek A,Hamilton A,Rouault-Pierre K,Abarrategi A,Albornoz MG,Miraki-Moud F,Bah N,Gribben J,Fitzgibbon J,Taussig D,Bonnet D

Mon Jun 01 00:00:00 EDT 2020

A stress-responsive enhancer induces dynamic drug resistance in acute myeloid leukemia.

The Journal of clinical investigation

Williams MS,Amaral FM,Simeoni F,Somervaille TC

Mon Mar 02 00:00:00 EST 2020

Real-Time NMR Spectroscopy for Studying Metabolism.

Angewandte Chemie (International ed. in English)

Alshamleh I,Krause N,Richter C,Kurrle N,Serve H,Günther UL,Schwalbe H

Mon Feb 03 00:00:00 EST 2020

Metabolic Regulation of Inflammasome Activity Controls Embryonic Hematopoietic Stem and Progenitor Cell Production.

Developmental cell

Frame JM,Kubaczka C,Long TL,Esain V,Soto RA,Hachimi M,Jing R,Shwartz A,Goessling W,Daley GQ,North TE

300-18 was used in In vitro experiments to establish the inflammasome as a conserved metabolic sensor that expands HSPC production in vivo and in vitro.

Mon Oct 26 00:00:00 EDT 2020

Efficient and nontoxic biomolecule delivery to primary human hematopoietic stem cells using nanostraws.

Proceedings of the National Academy of Sciences of the United States of America

Schmiderer L,Subramaniam A,Žemaitis K,Bäckström A,Yudovich D,Soboleva S,Galeev R,Prinz CN,Larsson J,Hjort M

Tue Sep 01 00:00:00 EDT 2020

Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells.

Proceedings of the National Academy of Sciences of the United States of America

Belling JN,Heidenreich LK,Tian Z,Mendoza AM,Chiou TT,Gong Y,Chen NY,Young TD,Wattanatorn N,Park JH,Scarabelli L,Chiang N,Takahashi J,Young SG,Stieg AZ,De Oliveira S,Huang TJ,Weiss PS,Jonas SJ

Tue May 19 00:00:00 EDT 2020

Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway.

Proceedings of the National Academy of Sciences of the United States of America

Wang T,Medynets M,Johnson KR,Doucet-O'Hare TT,DiSanza B,Li W,Xu Y,Bagnell A,Tyagi R,Sampson K,Malik N,Steiner J,Hadegan A,Kowalak J,O'Malley J,Maric D,Nath A

Tue Jul 28 00:00:00 EDT 2020

Machine Learning of Hematopoietic Stem Cell Divisions from Paired Daughter Cell Expression Profiles Reveals Effects of Aging on Self-Renewal.

Cell systems

Arai F,Stumpf PS,Ikushima YM,Hosokawa K,Roch A,Lutolf MP,Suda T,MacArthur BD

300-18 was used in Cell Culture to indicate that the balance between intrinsic and extrinsic regulation of stem cell activity alters substantially with age and help explain why stem cell numbers increase through life, yet regenerative potency declines.

Wed Dec 16 00:00:00 EST 2020

Heme oxygenase-1 deficiency triggers exhaustion of hematopoietic stem cells.

EMBO reports

Szade K,Zukowska M,Szade A,Nowak W,Skulimowska I,Ciesla M,Bukowska-Strakova K,Gulati GS,Kachamakova-Trojanowska N,Kusienicka A,Einwallner E,Kijowski J,Czauderna S,Esterbauer H,Benes V,L Weissman I,Dulak J,Jozkowicz A

Wed Feb 05 00:00:00 EST 2020

Heme oxygenase-1 deficiency triggers exhaustion of hematopoietic stem cells.

EMBO reports

Wed Feb 05 00:00:00 EST 2020

ZBTB7A prevents RUNX1-RUNX1T1-dependent clonal expansion of human hematopoietic stem and progenitor cells.

Oncogene

Redondo Monte E,Wilding A,Leubolt G,Kerbs P,Bagnoli JW,Hartmann L,Hiddemann W,Chen-Wichmann L,Krebs S,Blum H,Cusan M,Vick B,Jeremias I,Enard W,Theurich S,Wichmann C,Greif PA

Wed Apr 01 00:00:00 EDT 2020

Combination of a mitogen-activated protein kinase inhibitor with the tyrosine kinase inhibitor pacritinib combats cell adhesion-based residual disease and prevents re-expansion of FLT3-ITD acute myeloid leukaemia.

British journal of haematology

Zabkiewicz J,Lazenby M,Edwards G,Bygrave CA,Omidvar N,Zhuang L,Knapper S,Guy C,Hills RK,Burnett AK,Alvares CL

Thu Oct 01 00:00:00 EDT 2020

Coinhibition of activated p38 MAPKα and mTORC1 potentiates stemness maintenance of HSCs from SR1-expanded human cord blood CD34⁺ cells via inhibition of senescence.

Stem cells translational medicine

Li X,Ma X,Chen Y,Peng D,Wang H,Chen S,Xiao Y,Li L,Zhou H,Cheng F,Gao Y,Chang J,Cheng T,Liu L

Tue Dec 01 00:00:00 EST 2020

Regulation of ABO blood group antigen expression by miR-331-3p and miR-1908-5p during hematopoietic stem cell differentiation.

Stem cells (Dayton, Ohio)

Kronstein-Wiedemann R,Nowakowska P,Milanov P,Gubbe K,Seifried E,Bugert P,Chavakis T,Tonn T

Thu Oct 01 00:00:00 EDT 2020

EDAG mediates Hsp70 nuclear localization in erythroblasts and rescues dyserythropoiesis in myelodysplastic syndrome.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Dong XM,Zhao K,Zheng WW,Xu CW,Zhang MJ,Yin RH,Gao R,Tang LJ,Liu JF,Chen H,Zhan YQ,Yu M,Ge CH,Gao HY,Li X,Luo T,Ning HM,Yang XM,Li CY

Mon Jun 01 00:00:00 EDT 2020

Good Manufacturing Practice-Grade of Megakaryocytes Produced by a Novel Ex Vivo Culturing Platform.

Clinical and translational science

Guan X,Wang L,Wang H,Wang H,Dai W,Jiang Y

Sun Nov 01 00:00:00 EDT 2020

Lentiviral gene therapy rescues p47^phox chronic granulomatous disease and the ability to fight Salmonella infection in mice.

Gene therapy

Schejtman A,Aragão-Filho WC,Clare S,Zinicola M,Weisser M,Burns SO,Booth C,Gaspar HB,Thomas DC,Condino-Neto A,Thrasher AJ,Santilli G

Tue Sep 01 00:00:00 EDT 2020

Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome.

Nature communications

Rai R,Romito M,Rivers E,Turchiano G,Blattner G,Vetharoy W,Ladon D,Andrieux G,Zhang F,Zinicola M,Leon-Rico D,Santilli G,Thrasher AJ,Cavazza A

Wed Aug 12 00:00:00 EDT 2020

Nuclear interacting SET domain protein 1 inactivation impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

Nature communications

Leonards K,Almosailleakh M,Tauchmann S,Bagger FO,Thirant C,Juge S,Bock T,Méreau H,Bezerra MF,Tzankov A,Ivanek R,Losson R,Peters AHFM,Mercher T,Schwaller J

Fri Jun 12 00:00:00 EDT 2020

Nuclear interacting SET domain protein 1 inactivation impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

Nature communications

Leonards K,Almosailleakh M,Tauchmann S,Bagger FO,Thirant C,Juge S,Bock T,Méreau H,Bezerra MF,Tzankov A,Ivanek R,Losson R,Peters AHFM,Mercher T,Schwaller J

Fri Jun 12 00:00:00 EDT 2020

Nuclear interacting SET domain protein 1 inactivation impairs GATA1-regulated erythroid differentiation and causes erythroleukemia.

Nature communications

Leonards K,Almosailleakh M,Tauchmann S,Bagger FO,Thirant C,Juge S,Bock T,Méreau H,Bezerra MF,Tzankov A,Ivanek R,Losson R,Peters AHFM,Mercher T,Schwaller J

Fri Jun 12 00:00:00 EDT 2020

Inducible Forward Programming of Human Pluripotent Stem Cells to Hemato-endothelial Progenitor Cells with Hematopoietic Progenitor Potential.

Stem cell reports

Lange L,Hoffmann D,Schwarzer A,Ha TC,Philipp F,Lenz D,Morgan M,Schambach A

Tue Jan 14 00:00:00 EST 2020

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.

Stem cell reports

Suzuki D,Flahou C,Yoshikawa N,Stirblyte I,Hayashi Y,Sawaguchi A,Akasaka M,Nakamura S,Higashi N,Xu H,Matsumoto T,Fujio K,Manz MG,Hotta A,Takizawa H,Eto K,Sugimoto N

Tue Jan 14 00:00:00 EST 2020

Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-β Signaling.

Cell reports

Sinha S,Biswas M,Chatterjee SS,Kumar S,Sengupta A

300-18 was used in Cell Culture to study the molecular integration of developmental signaling with mesenchymal-stromal-cell (MSC) intrinsic chromatin regulation. To reveal a link illustrating epi-genomic feedforward control of bone morphogenic protein (BMP)/transforming growth factor β (TGF-β) signaling to transcriptional and cellular plasticity in the mesenchymal microenvironment and account for stromal-SWI/SNF in hematopoiesis.

Tue Apr 28 00:00:00 EDT 2020

Human CLEC9A antibodies deliver Wilms' tumor 1 (WT1) antigen to CD141⁺ dendritic cells to activate naïve and memory WT1-specific CD8⁺ T cells.

Clinical & translational immunology

Pearson FE,Tullett KM,Leal-Rojas IM,Haigh OL,Masterman KA,Walpole C,Bridgeman JS,McLaren JE,Ladell K,Miners K,Llewellyn-Lacey S,Price DA,Tunger A,Schmitz M,Miles JJ,Lahoud MH,Radford KJ

Fri Apr 15 00:00:00 EDT 2022

Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype.

Nature communications

Thornton N,Karamatic Crew V,Tilley L,Green CA,Tay CL,Griffiths RE,Singleton BK,Spring F,Walser P,Alattar AG,Jones B,Laundy R,Storry JR,Möller M,Wall L,Charlewood R,Westhoff CM,Lomas-Francis C,Yahalom V,Feick U,Seltsam A,Mayer B,Olsson ML,Anstee DJ

Thu Jul 16 00:00:00 EDT 2020

Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Osteoclasts.

Bio-protocol

Chen IP

300-18 was used in Cell Culture to study efficient differentiation of hiPSCs into osteoclasts using a three-stage protocol which does not require a co-culture system; induces EBs into mesoderm differentiation in a homogenous manner; uses less cytokines for differentiation; requires only a short time for osteoclast maturation and produces sufficient numbers of osteoclasts for subsequent molecular analyses.

Sun Dec 20 00:00:00 EST 2020

Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System.

Molecular therapy. Methods & clinical development

Shapiro J,Iancu O,Jacobi AM,McNeill MS,Turk R,Rettig GR,Amit I,Tovin-Recht A,Yakhini Z,Behlke MA,Hendel A

Fri Jun 12 00:00:00 EDT 2020

Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency.

Nature communications

Araki R,Hoki Y,Suga T,Obara C,Sunayama M,Imadome K,Fujita M,Kamimura S,Nakamura M,Wakayama S,Nagy A,Wakayama T,Abe M

Fri Jan 10 00:00:00 EST 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

300-18 was used in In vitro experiments to report a disease modeling platform to study the role of macrophages in patients with inborn errors of IFN-γ immunity.

Wed Feb 19 00:00:00 EST 2020

Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V.

Nature communications

Rai S,Tanaka H,Suzuki M,Espinoza JL,Kumode T,Tanimura A,Yokota T,Oritani K,Watanabe T,Kanakura Y,Matsumura I

Tue Aug 18 00:00:00 EDT 2020

Establishment of a Long-Term Co-culture Assay for Mesenchymal Stromal Cells and Hematopoietic Stem/Progenitors.

STAR protocols

Sinha S,Chakraborty S,Sengupta A

Fri Dec 18 00:00:00 EST 2020

Single-Cell RNA Sequencing Resolves Spatiotemporal Development of Pre-thymic Lymphoid Progenitors and Thymus Organogenesis in Human Embryos.

Immunity

Zeng Y,Liu C,Gong Y,Bai Z,Hou S,He J,Bian Z,Li Z,Ni Y,Yan J,Huang T,Shi H,Ma C,Chen X,Wang J,Bian L,Lan Y,Liu B,Hu H

Tue Nov 19 00:00:00 EST 2019

Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.

Cancer cell

Huang Y,Su R,Sheng Y,Dong L,Dong Z,Xu H,Ni T,Zhang ZS,Zhang T,Li C,Han L,Zhu Z,Lian F,Wei J,Deng Q,Wang Y,Wunderlich M,Gao Z,Pan G,Zhong D,Zhou H,Zhang N,Gan J,Jiang H,Mulloy JC,Qian Z,Chen J,Yang CG

Mon Apr 15 00:00:00 EDT 2019

Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.

Cancer cell

Huang Y,Su R,Sheng Y,Dong L,Dong Z,Xu H,Ni T,Zhang ZS,Zhang T,Li C,Han L,Zhu Z,Lian F,Wei J,Deng Q,Wang Y,Wunderlich M,Gao Z,Pan G,Zhong D,Zhou H,Zhang N,Gan J,Jiang H,Mulloy JC,Qian Z,Chen J,Yang CG

Mon Apr 15 00:00:00 EDT 2019

Mechanisms of Progression of Myeloid Preleukemia to Transformed Myeloid Leukemia in Children with Down Syndrome.

Cancer cell

Labuhn M,Perkins K,Matzk S,Varghese L,Garnett C,Papaemmanuil E,Metzner M,Kennedy A,Amstislavskiy V,Risch T,Bhayadia R,Samulowski D,Hernandez DC,Stoilova B,Iotchkova V,Oppermann U,Scheer C,Yoshida K,Schwarzer A,Taub JW,Crispino JD,Weiss MJ,Hayashi Y,Taga T,Ito E,Ogawa S,Reinhardt D,Yaspo ML,Campbell PJ,Roberts I,Constantinescu SN,Vyas P,Heckl D,Klusmann JH

Mon Aug 12 00:00:00 EDT 2019

PRDM16s transforms megakaryocyte-erythroid progenitors into myeloid leukemia-initiating cells.

Blood

Hu T,Morita K,Hill MC,Jiang Y,Kitano A,Saito Y,Wang F,Mao X,Hoegenauer KA,Morishita K,Martin JF,Futreal PA,Takahashi K,Nakada D

Thu Aug 15 00:00:00 EDT 2019

Germline mutations in the transcription factor IKZF5 cause thrombocytopenia.

Blood

Lentaigne C,Greene D,Sivapalaratnam S,Favier R,Seyres D,Thys C,Grassi L,Mangles S,Sibson K,Stubbs M,Burden F,Bordet JC,Armari-Alla C,Erber W,Farrow S,Gleadall N,Gomez K,Megy K,Papadia S,Penkett CJ,Sims MC,Stefanucci L,Stephens JC,Read RJ,Stirrups KE,Ouwehand WH,Laffan MA,Frontini M,Freson K,Turro E

Thu Dec 05 00:00:00 EST 2019

The BRISC deubiquitinating enzyme complex limits hematopoietic stem cell expansion by regulating JAK2 K63-ubiquitination.

Blood

Donaghy R,Han X,Rozenova K,Lv K,Jiang Q,Doepner M,Greenberg RA,Tong W

Thu Apr 04 00:00:00 EDT 2019

The BRISC deubiquitinating enzyme complex limits hematopoietic stem cell expansion by regulating JAK2 K63-ubiquitination.

Blood

Donaghy R,Han X,Rozenova K,Lv K,Jiang Q,Doepner M,Greenberg RA,Tong W

Thu Apr 04 00:00:00 EDT 2019

IL-7R is essential for leukemia-initiating cell activity of T-cell acute lymphoblastic leukemia.

Blood

González-García S,Mosquera M,Fuentes P,Palumbo T,Escudero A,Pérez-Martínez A,Ramírez M,Corcoran AE,Toribio ML

Thu Dec 12 00:00:00 EST 2019

Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer.

Cell stem cell

Zhu Y,Smith DJ,Zhou Y,Li YR,Yu J,Lee D,Wang YC,Di Biase S,Wang X,Hardoy C,Ku J,Tsao T,Lin LJ,Pham AT,Moon H,McLaughlin J,Cheng D,Hollis RP,Campo-Fernandez B,Urbinati F,Wei L,Pang L,Rezek V,Berent-Maoz B,Macabali MH,Gjertson D,Wang X,Galic Z,Kitchen SG,An DS,Hu-Lieskovan S,Kaplan-Lefko PJ,De Oliveira SN,Seet CS,Larson SM,Forman SJ,Heath JR,Zack JA,Crooks GM,Radu CG,Ribas A,Kohn DB,Witte ON,Yang L

300-18 was used in In vitro experiments to develop a proof-of-concept for hematopoietic stem cell-engineered invariant natural killer T (iNKT) cell therapy with the potential to provide therapeutic levels of iNKT cells for a patient's lifetime.

Thu Oct 03 00:00:00 EDT 2019

Remodeling of Bone Marrow Hematopoietic Stem Cell Niches Promotes Myeloid Cell Expansion during Premature or Physiological Aging.

Cell stem cell

Ho YH,Del Toro R,Rivera-Torres J,Rak J,Korn C,García-García A,Macías D,González-Gómez C,Del Monte A,Wittner M,Waller AK,Foster HR,López-Otín C,Johnson RS,Nerlov C,Ghevaert C,Vainchenker W,Louache F,Andrés V,Méndez-Ferrer S

300-18 was used in Cell Culture to show that the bone marrow microenvironment promotes myelopoiesis in premature/physiological aging.

Thu Sep 05 00:00:00 EDT 2019

Spatial Chromosome Folding and Active Transcription Drive DNA Fragility and Formation of Oncogenic MLL Translocations.

Molecular cell

Gothe HJ,Bouwman BAM,Gusmao EG,Piccinno R,Petrosino G,Sayols S,Drechsel O,Minneker V,Josipovic N,Mizi A,Nielsen CF,Wagner EM,Takeda S,Sasanuma H,Hudson DF,Kindler T,Baranello L,Papantonis A,Crosetto N,Roukos V

300-18 was used in Cell Culture to propose that transcription and 3D chromosome folding jointly pose a threat to genomic stability and are key contributors to the occurrence of genome rearrangements that drive cancer.

Thu Jul 25 00:00:00 EDT 2019

Inhibition of mitochondrial translation overcomes venetoclax resistance in AML through activation of the integrated stress response.

Science translational medicine

Sharon D,Cathelin S,Mirali S,Di Trani JM,Yanofsky DJ,Keon KA,Rubinstein JL,Schimmer AD,Ketela T,Chan SM

300-18 was used in Cell Culture to investigate if inactivation of genes involved in mitochondrial translation restores sensitivity to venetoclax in resistant acute myeloid leukemia cells.

Wed Oct 30 00:00:00 EDT 2019

Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo.

Neuron

Hasselmann J,Coburn MA,England W,Figueroa Velez DX,Kiani Shabestari S,Tu CH,McQuade A,Kolahdouzan M,Echeverria K,Claes C,Nakayama T,Azevedo R,Coufal NG,Han CZ,Cummings BJ,Davtyan H,Glass CK,Healy LM,Gandhi SP,Spitale RC,Blurton-Jones M

Wed Sep 25 00:00:00 EDT 2019

Enabling Large-Scale Ex Vivo Production of Megakaryocytes from CD34⁺ Cells Using Gas-Permeable Surfaces.

Stem cells translational medicine

Martinez AF,Miller WM

Mon Jul 01 00:00:00 EDT 2019

Putative Role of Nuclear Factor-Kappa B But Not Hypoxia-Inducible Factor-1α in Hypoxia-Dependent Regulation of Oxidative Stress in Hematopoietic Stem and Progenitor Cells.

Antioxidants & redox signaling

Halvarsson C,Rörby E,Eliasson P,Lang S,Soneji S,Jönsson JI

300-18 was used in Cell Culture to investigate if HIF-1α protects hematopoietic stem cells against ROS.

Sat Jul 20 00:00:00 EDT 2019

Improving Gene Editing Outcomes in Human Hematopoietic Stem and Progenitor Cells by Temporal Control of DNA Repair.

Stem cells (Dayton, Ohio)

Lomova A,Clark DN,Campo-Fernandez B,Flores-Bjurström C,Kaufman ML,Fitz-Gibbon S,Wang X,Miyahira EY,Brown D,DeWitt MA,Corn JE,Hollis RP,Romero Z,Kohn DB

Fri Feb 01 00:00:00 EST 2019

Paracrine Factors Released by Osteoblasts Provide Strong Platelet Engraftment Properties.

Stem cells (Dayton, Ohio)

Abu-Khader A,Law KW,Jahan S,Manesia JK,Pasha R,Hovey O,Pineault N

Fri Mar 01 00:00:00 EST 2019

Efficient gene correction of an aberrant splice site in β-thalassaemia iPSCs by CRISPR/Cas9 and single-strand oligodeoxynucleotides.

Journal of cellular and molecular medicine

Xiong Z,Xie Y,Yang Y,Xue Y,Wang D,Lin S,Chen D,Lu D,He L,Song B,Yang Y,Sun X

Sun Dec 01 00:00:00 EST 2019

Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity.

Cell reports

Aho ER,Wang J,Gogliotti RD,Howard GC,Phan J,Acharya P,Macdonald JD,Cheng K,Lorey SL,Lu B,Wenzel S,Foshage AM,Alvarado J,Wang F,Shaw JG,Zhao B,Weissmiller AM,Thomas LR,Vakoc CR,Hall MD,Hiebert SW,Liu Q,Stauffer SR,Fesik SW,Tansey WP

300-18 was used in Cell Culture to identify WIN site inhibitors and demonstrate that the WIN site links WDR5 to chromatin at a small cohort of loci, including a specific subset of ribosome protein genes.

Tue Mar 12 00:00:00 EDT 2019

Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis.

Cell death & disease

Zhou Y,Zhang B,Li C,Huang X,Cheng H,Bao X,Zhao F,Cheng Q,Yue S,Han J,Luo Z

Mon Sep 09 00:00:00 EDT 2019

Mebendazole for Differentiation Therapy of Acute Myeloid Leukemia Identified by a Lineage Maturation Index.

Scientific reports

Li Y,Thomas D,Deutzmann A,Majeti R,Felsher DW,Dill DL

Thu Nov 14 00:00:00 EST 2019

The signaling axis atypical protein kinase C λ/ι-Satb2 mediates leukemic transformation of B-cell progenitors.

Nature communications

Nayak RC,Hegde S,Althoff MJ,Wellendorf AM,Mohmoud F,Perentesis J,Reina-Campos M,Reynaud D,Zheng Y,Diaz-Meco MT,Moscat J,Cancelas JA

Fri Jan 04 00:00:00 EST 2019

A comprehensive single cell transcriptional landscape of human hematopoietic progenitors.

Nature communications

Pellin D,Loperfido M,Baricordi C,Wolock SL,Montepeloso A,Weinberg OK,Biffi A,Klein AM,Biasco L

Mon Jun 03 00:00:00 EDT 2019

Human multipotent hematopoietic progenitor cell expansion is neither supported in endothelial and endothelial/mesenchymal co-cultures nor in NSG mice.

Scientific reports

Radtke S,Görgens A,da Conceição Castro SV,Kordelas L,Köninger A,Dürig J,Möllmann M,Horn PA,Giebel B

Mon Sep 09 00:00:00 EDT 2019

Lentiviral Gene Therapy in HSCs Restores Lineage-Specific Foxp3 Expression and Suppresses Autoimmunity in a Mouse Model of IPEX Syndrome.

Cell stem cell

Masiuk KE,Laborada J,Roncarolo MG,Hollis RP,Kohn DB

300-18 was used in Cell Culture to demonstrate preclinical efficacy for the treatment of IPEX patients by autologous HSC transplant and may provide valuable insights into new cell therapies for autoimmunity.

Thu Feb 07 00:00:00 EST 2019

Synthetic modeling reveals HOXB genes are critical for the initiation and maintenance of human leukemia.

Nature communications

Kusakabe M,Sun AC,Tyshchenko K,Wong R,Nanda A,Shanna C,Gusscott S,Chavez EA,Lorzadeh A,Zhu A,Hill A,Hung S,Brown S,Babaian A,Wang X,Holt RA,Steidl C,Karsan A,Humphries RK,Eaves CJ,Hirst M,Weng AP

Tue Jul 02 00:00:00 EDT 2019

The long non-coding RNA HOXB-AS3 regulates ribosomal RNA transcription in NPM1-mutated acute myeloid leukemia.

Nature communications

Papaioannou D,Petri A,Dovey OM,Terreri S,Wang E,Collins FA,Woodward LA,Walker AE,Nicolet D,Pepe F,Kumchala P,Bill M,Walker CJ,Karunasiri M,Mrózek K,Gardner ML,Camilotto V,Zitzer N,Cooper JL,Cai X,Rong-Mullins X,Kohlschmidt J,Archer KJ,Freitas MA,Zheng Y,Lee RJ,Aifantis I,Vassiliou G,Singh G,Kauppinen S,Bloomfield CD,Dorrance AM,Garzon R

Mon Nov 25 00:00:00 EST 2019

Targeting of apoptosis gene loci by reprogramming factors leads to selective eradication of leukemia cells.

Nature communications

Wang Y,Lu T,Sun G,Zheng Y,Yang S,Zhang H,Hao S,Liu Y,Ma S,Zhang H,Ru Y,Gao S,Yen K,Cheng H,Cheng T

Fri Dec 06 00:00:00 EST 2019

Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells.

Nature

Carrelha J,Meng Y,Kettyle LM,Luis TC,Norfo R,Alcolea V,Boukarabila H,Grasso F,Gambardella A,Grover A,Högstrand K,Lord AM,Sanjuan-Pla A,Woll PS,Nerlov C,Jacobsen SEW

Thu Feb 01 00:00:00 EST 2018

Identification of the Human Skeletal Stem Cell.

Cell

Chan CKF,Gulati GS,Sinha R,Tompkins JV,Lopez M,Carter AC,Ransom RC,Reinisch A,Wearda T,Murphy M,Brewer RE,Koepke LS,Marecic O,Manjunath A,Seo EY,Leavitt T,Lu WJ,Nguyen A,Conley SD,Salhotra A,Ambrosi TH,Borrelli MR,Siebel T,Chan K,Schallmoser K,Seita J,Sahoo D,Goodnough H,Bishop J,Gardner M,Majeti R,Wan DC,Goodman S,Weissman IL,Chang HY,Longaker MT

Thu Sep 20 00:00:00 EDT 2018

CBFβ-SMMHC Inhibition Triggers Apoptosis by Disrupting MYC Chromatin Dynamics in Acute Myeloid Leukemia.

Cell

Pulikkan JA,Hegde M,Ahmad HM,Belaghzal H,Illendula A,Yu J,O'Hagan K,Ou J,Muller-Tidow C,Wolfe SA,Zhu LJ,Dekker J,Bushweller JH,Castilla LH

Thu Jun 28 00:00:00 EDT 2018

Pseudouridylation of tRNA-Derived Fragments Steers Translational Control in Stem Cells.

Cell

Guzzi N,Cieśla M,Ngoc PCT,Lang S,Arora S,Dimitriou M,Pimková K,Sommarin MNE,Munita R,Lubas M,Lim Y,Okuyama K,Soneji S,Karlsson G,Hansson J,Jönsson G,Lund AH,Sigvardsson M,Hellström-Lindberg E,Hsieh AC,Bellodi C

Thu May 17 00:00:00 EDT 2018

R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m⁶A/MYC/CEBPA Signaling.

Cell

Su R,Dong L,Li C,Nachtergaele S,Wunderlich M,Qing Y,Deng X,Wang Y,Weng X,Hu C,Yu M,Skibbe J,Dai Q,Zou D,Wu T,Yu K,Weng H,Huang H,Ferchen K,Qin X,Zhang B,Qi J,Sasaki AT,Plas DR,Bradner JE,Wei M,Marcucci G,Jiang X,Mulloy JC,Jin J,He C,Chen J

Thu Jan 11 00:00:00 EST 2018

Small Molecules Co-targeting CKIα and the Transcriptional Kinases CDK7/9 Control AML in Preclinical Models.

Cell

Minzel W,Venkatachalam A,Fink A,Hung E,Brachya G,Burstain I,Shaham M,Rivlin A,Omer I,Zinger A,Elias S,Winter E,Erdman PE,Sullivan RW,Fung L,Mercurio F,Li D,Vacca J,Kaushansky N,Shlush L,Oren M,Levine R,Pikarsky E,Snir-Alkalay I,Ben-Neriah Y

Thu Sep 20 00:00:00 EDT 2018

Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia.

Cell

Kim MY,Yu KR,Kenderian SS,Ruella M,Chen S,Shin TH,Aljanahi AA,Schreeder D,Klichinsky M,Shestova O,Kozlowski MS,Cummins KD,Shan X,Shestov M,Bagg A,Morrissette JJD,Sekhri P,Lazzarotto CR,Calvo KR,Kuhns DB,Donahue RE,Behbehani GK,Tsai SQ,Dunbar CE,Gill S

Thu May 31 00:00:00 EDT 2018

Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia.

Cell

Thu May 31 00:00:00 EDT 2018

Ezh2 and Runx1 Mutations Collaborate to Initiate Lympho-Myeloid Leukemia in Early Thymic Progenitors.

Cancer cell

Booth CAG,Barkas N,Neo WH,Boukarabila H,Soilleux EJ,Giotopoulos G,Farnoud N,Giustacchini A,Ashley N,Carrelha J,Jamieson L,Atkinson D,Bouriez-Jones T,Prinjha RK,Milne TA,Teachey DT,Papaemmanuil E,Huntly BJP,Jacobsen SEW,Mead AJ

Mon Feb 12 00:00:00 EST 2018

Human NOTCH4 is a key target of RUNX1 in megakaryocytic differentiation.

Blood

Li Y,Jin C,Bai H,Gao Y,Sun S,Chen L,Qin L,Liu PP,Cheng L,Wang QF

Thu Jan 11 00:00:00 EST 2018

IGF-1 facilitates thrombopoiesis primarily through Akt activation.

Blood

Chen S,Hu M,Shen M,Wang S,Wang C,Chen F,Tang Y,Wang X,Zeng H,Chen M,Gao J,Wang F,Su Y,Xu Y,Wang J

Thu Jul 12 00:00:00 EDT 2018

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

300-18 was used in In vitro experiments to report on the involvement of innate pathogen recognition molecules in immune blocks to gene correction in primary human hematopoietic stem cells.

Thu Dec 06 00:00:00 EST 2018

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Cell stem cell

Petrillo C,Thorne LG,Unali G,Schiroli G,Giordano AMS,Piras F,Cuccovillo I,Petit SJ,Ahsan F,Noursadeghi M,Clare S,Genovese P,Gentner B,Naldini L,Towers GJ,Kajaste-Rudnitski A

300-18 was used in In vitro experiments to report on the involvement of innate pathogen recognition molecules in immune blocks to gene correction in primary human hematopoietic stem cells.

Thu Dec 06 00:00:00 EST 2018

Iron modulation of erythropoiesis is associated with Scribble-mediated control of the erythropoietin receptor.

The Journal of experimental medicine

Khalil S,Delehanty L,Grado S,Holy M,White Z,Freeman K,Kurita R,Nakamura Y,Bullock G,Goldfarb A

Mon Feb 05 00:00:00 EST 2018

A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1.

Developmental cell

Böiers C,Richardson SE,Laycock E,Zriwil A,Turati VA,Brown J,Wray JP,Wang D,James C,Herrero J,Sitnicka E,Karlsson S,Smith AJH,Jacobsen SEW,Enver T

300-18 was used in Cell Culture to explore the link between embryonic B-myeloid fate restriction and ETV6-RUNX1 mutant acute B-lymphoblastic leukaemia development.

Mon Feb 05 00:00:00 EST 2018

Quality-Quantity Control Culture Enhances Vasculogenesis and Wound Healing Efficacy of Human Diabetic Peripheral Blood CD34+ Cells.

Stem cells translational medicine

Tanaka R,Masuda H,Fujimura S,Ito-Hirano R,Arita K,Kakinuma Y,Hagiwara H,Kado M,Hayashi A,Mita T,Ogawa T,Watada H,Mizuno H,Sawada N,Asahara T

Tue May 01 00:00:00 EDT 2018

X-linked inhibitor of apoptosis inhibition sensitizes acute myeloid leukemia cell response to TRAIL and chemotherapy through potentiated induction of proapoptotic machinery.

Molecular oncology

Zhou J,Lu X,Tan TZ,Chng WJ

Mon Jan 01 00:00:00 EST 2018

MEIS1 Regulates Hemogenic Endothelial Generation, Megakaryopoiesis, and Thrombopoiesis in Human Pluripotent Stem Cells by Targeting TAL1 and FLI1.

Stem cell reports

Wang H,Liu C,Liu X,Wang M,Wu D,Gao J,Su P,Nakahata T,Zhou W,Xu Y,Shi L,Ma F,Zhou J

Tue Feb 13 00:00:00 EST 2018

Somatic Mutations Reveal Lineage Relationships and Age-Related Mutagenesis in Human Hematopoiesis.

Cell reports

Osorio FG,Rosendahl Huber A,Oka R,Verheul M,Patel SH,Hasaart K,de la Fonteijne L,Varela I,Camargo FD,van Boxtel R

300-18 was used in Cell Culture to perform genomic analysis of the mutation accumulation of HSPCs.

Tue Nov 27 00:00:00 EST 2018

NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells.

Nature communications

Uenishi GI,Jung HS,Kumar A,Park MA,Hadland BK,McLeod E,Raymond M,Moskvin O,Zimmerman CE,Theisen DJ,Swanson S,J Tamplin O,Zon LI,Thomson JA,Bernstein ID,Slukvin II

Tue May 08 00:00:00 EDT 2018

P38α/JNK signaling restrains erythropoiesis by suppressing Ezh2-mediated epigenetic silencing of Bim.

Nature communications

Hu P,Nebreda AR,Hanenberg H,Kinnebrew GH,Ivan M,Yoder MC,Filippi MD,Broxmeyer HE,Kapur R

Wed Aug 29 00:00:00 EDT 2018

Consequences of cathepsin C inactivation for membrane exposure of proteinase 3, the target antigen in autoimmune vasculitis.

The Journal of biological chemistry

Seren S,Rashed Abouzaid M,Eulenberg-Gustavus C,Hirschfeld J,Nasr Soliman H,Jerke U,N'Guessan K,Dallet-Choisy S,Lesner A,Lauritzen C,Schacher B,Eickholz P,Nagy N,Szell M,Croix C,Viaud-Massuard MC,Al Farraj Aldosari A,Ragunatha S,Ibrahim Mostafa M,Giampieri F,Battino M,Cornillier H,Lorette G,Stephan JL,Goizet C,Pedersen J,Gauthier F,Jenne DE,Marchand-Adam S,Chapple IL,Kettritz R,Korkmaz B

Fri Aug 10 00:00:00 EDT 2018

Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity.

Cell stem cell

Giambra V,Gusscott S,Gracias D,Song R,Lam SH,Panelli P,Tyshchenko K,Jenkins CE,Hoofd C,Lorzadeh A,Carles A,Hirst M,Eaves CJ,Weng AP

300-18 was used in In vitro experiments to demonstrate that resurrecting dormant fetal programs in adult cells may represent an alternate therapeutic approach in human cancer.

Thu Nov 01 00:00:00 EDT 2018

Large-Scale Human Dendritic Cell Differentiation Revealing Notch-Dependent Lineage Bifurcation and Heterogeneity.

Cell reports

Balan S,Arnold-Schrauf C,Abbas A,Couespel N,Savoret J,Imperatore F,Villani AC,Vu Manh TP,Bhardwaj N,Dalod M

300-18 was used in Cell Culture to develop a dendritic cell (DC) differentiation method from human CD34+ precursors leading to high yields of plasmacytoid DCs and both types of conventional DCs.

Tue Aug 14 00:00:00 EDT 2018

Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function.

Nature communications

Laustsen A,Bak RO,Krapp C,Kjær L,Egedahl JH,Petersen CC,Pillai S,Tang HQ,Uldbjerg N,Porteus M,Roan NR,Nyegaard M,Denton PW,Jakobsen MR

Thu Aug 30 00:00:00 EDT 2018

Convergent Pathways in Idiopathic Autism Revealed by Time Course Transcriptomic Analysis of Patient-Derived Neurons.

Scientific reports

DeRosa BA,El Hokayem J,Artimovich E,Garcia-Serje C,Phillips AW,Van Booven D,Nestor JE,Wang L,Cuccaro ML,Vance JM,Pericak-Vance MA,Cukier HN,Nestor MW,Dykxhoorn DM

Wed May 30 00:00:00 EDT 2018

Large-scale production of megakaryocytes in microcarrier-supported stirred suspension bioreactors.

Scientific reports

Eicke D,Baigger A,Schulze K,Latham SL,Halloin C,Zweigerdt R,Guzman CA,Blasczyk R,Figueiredo C

Thu Jul 05 00:00:00 EDT 2018

Early Human Hemogenic Endothelium Generates Primitive and Definitive Hematopoiesis In Vitro.

Stem cell reports

Garcia-Alegria E,Menegatti S,Fadlullah MZH,Menendez P,Lacaud G,Kouskoff V

Tue Nov 13 00:00:00 EST 2018

A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.

Nature communications

Sheng C,Jungverdorben J,Wiethoff H,Lin Q,Flitsch LJ,Eckert D,Hebisch M,Fischer J,Kesavan J,Weykopf B,Schneider L,Holtkamp D,Beck H,Till A,Wüllner U,Ziller MJ,Wagner W,Peitz M,Brüstle O

Tue Oct 02 00:00:00 EDT 2018

Reconstruction of complex single-cell trajectories using CellRouter.

Nature communications

Lummertz da Rocha E,Rowe RG,Lundin V,Malleshaiah M,Jha DK,Rambo CR,Li H,North TE,Collins JJ,Daley GQ

Thu Mar 01 00:00:00 EST 2018

Attenuated DNA damage responses and increased apoptosis characterize human hematopoietic stem cells exposed to irradiation.

Scientific reports

Biechonski S,Olender L,Zipin-Roitman A,Yassin M,Aqaqe N,Marcu-Malina V,Rall-Scharpf M,Trottier M,Meyn MS,Wiesmüller L,Beider K,Raz Y,Grisaru D,Nagler A,Milyavsky M

Tue Apr 17 00:00:00 EDT 2018

Targeting the Extracellular Signal-Regulated Kinase 5 Pathway to Suppress Human Chronic Myeloid Leukemia Stem Cells.

Stem cell reports

Tusa I,Cheloni G,Poteti M,Gozzini A,DeSouza NH,Shan Y,Deng X,Gray NS,Li S,Rovida E,Dello Sbarba P

Tue Oct 09 00:00:00 EDT 2018

iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors.

Cell death & disease

Melguizo-Sanchis D,Xu Y,Taheem D,Yu M,Tilgner K,Barta T,Gassner K,Anyfantis G,Wan T,Elango R,Alharthi S,El-Harouni AA,Przyborski S,Adam S,Saretzki G,Samarasinghe S,Armstrong L,Lako M

Fri Jan 26 00:00:00 EST 2018

Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia.

Nature communications

Latham SL,Ehmke N,Reinke PYA,Taft MH,Eicke D,Reindl T,Stenzel W,Lyons MJ,Friez MJ,Lee JA,Hecker R,Frühwald MC,Becker K,Neuhann TM,Horn D,Schrock E,Niehaus I,Sarnow K,Grützmann K,Gawehn L,Klink B,Rump A,Chaponnier C,Figueiredo C,Knöfler R,Manstein DJ,Di Donato N

Fri Oct 12 00:00:00 EDT 2018

Leukaemic alterations of IKZF1 prime stemness and malignancy programs in human lymphocytes.

Cell death & disease

Li Z,Li SP,Li RY,Zhu H,Liu X,Guo XL,Mu LL,Cai JJ,Bai F,Chen GQ,Hong DL

Tue May 01 00:00:00 EDT 2018

De novo activating mutations drive clonal evolution and enhance clonal fitness in KMT2A-rearranged leukemia.

Nature communications

Hyrenius-Wittsten A,Pilheden M,Sturesson H,Hansson J,Walsh MP,Song G,Kazi JU,Liu J,Ramakrishan R,Garcia-Ruiz C,Nance S,Gupta P,Zhang J,Rönnstrand L,Hultquist A,Downing JR,Lindkvist-Petersson K,Paulsson K,Järås M,Gruber TA,Ma J,Hagström-Andersson AK

Wed May 02 00:00:00 EDT 2018

Rapid Mast Cell Generation from Gata2 Reporter Pluripotent Stem Cells.

Stem cell reports

Kauts ML,De Leo B,Rodríguez-Seoane C,Ronn R,Glykofrydis F,Maglitto A,Kaimakis P,Basi M,Taylor H,Forrester L,Wilkinson AC,Göttgens B,Saunders P,Dzierzak E

Tue Oct 09 00:00:00 EDT 2018

Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia.

Nature communications

Balcerek J,Jiang J,Li Y,Jiang Q,Holdreith N,Singh B,Chandra V,Lv K,Ren JG,Rozenova K,Li W,Greenberg RA,Tong W

Tue Sep 25 00:00:00 EDT 2018

BCAT1 restricts αKG levels in AML stem cells leading to IDHmut-like DNA hypermethylation.

Nature

Raffel S,Falcone M,Kneisel N,Hansson J,Wang W,Lutz C,Bullinger L,Poschet G,Nonnenmacher Y,Barnert A,Bahr C,Zeisberger P,Przybylla A,Sohn M,Tönjes M,Erez A,Adler L,Jensen P,Scholl C,Fröhling S,Cocciardi S,Wuchter P,Thiede C,Flörcken A,Westermann J,Ehninger G,Lichter P,Hiller K,Hell R,Herrmann C,Ho AD,Krijgsveld J,Radlwimmer B,Trumpp A

Thu Nov 16 00:00:00 EST 2017

Physiologic Medium Rewires Cellular Metabolism and Reveals Uric Acid as an Endogenous Inhibitor of UMP Synthase.

Cell

Cantor JR,Abu-Remaileh M,Kanarek N,Freinkman E,Gao X,Louissaint A,Lewis CA,Sabatini DM

Thu Apr 06 00:00:00 EDT 2017

CpG Island Hypermethylation Mediated by DNMT3A Is a Consequence of AML Progression.

Cell

Spencer DH,Russler-Germain DA,Ketkar S,Helton NM,Lamprecht TL,Fulton RS,Fronick CC,O'Laughlin M,Heath SE,Shinawi M,Westervelt P,Payton JE,Wartman LD,Welch JS,Wilson RK,Walter MJ,Link DC,DiPersio JF,Ley TJ

Thu Feb 23 00:00:00 EST 2017

MLL2, Not MLL1, Plays a Major Role in Sustaining MLL-Rearranged Acute Myeloid Leukemia.

Cancer cell

Chen Y,Anastassiadis K,Kranz A,Stewart AF,Arndt K,Waskow C,Yokoyama A,Jones K,Neff T,Lee Y,Ernst P

Mon Jun 12 00:00:00 EDT 2017

Selinexor-induced thrombocytopenia results from inhibition of thrombopoietin signaling in early megakaryopoiesis.

Blood

Machlus KR,Wu SK,Vijey P,Soussou TS,Liu ZJ,Shacham E,Unger TJ,Kashyap T,Klebanov B,Sola-Visner M,Crochiere M,Italiano JE,Landesman Y

Thu Aug 31 00:00:00 EDT 2017

Clonal selection and asymmetric distribution of human leukemia in murine xenografts revealed by cellular barcoding.

Blood

Belderbos ME,Koster T,Ausema B,Jacobs S,Sowdagar S,Zwart E,de Bont E,de Haan G,Bystrykh LV

Thu Jun 15 00:00:00 EDT 2017

c-MPL provides tumor-targeted T-cell receptor-transgenic T cells with costimulation and cytokine signals.

Blood

Nishimura CD,Brenner DA,Mukherjee M,Hirsch RA,Ott L,Wu MF,Liu H,Dakhova O,Orange JS,Brenner MK,Lin CY,Arber C

Thu Dec 21 00:00:00 EST 2017

Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine.

Autophagy

Chen X,Clark J,Wunderlich M,Fan C,Davis A,Chen S,Guan JL,Mulloy JC,Kumar A,Zheng Y

300-18 was used in Cell Culture to uncover anti-leukemic effects of choloroquine being independent of autophagy which can be enhanced via vesicular exocytotis inhibition, and autophagy being an insignificant target of MA9-AML therapy.

Thu May 04 00:00:00 EDT 2017

Endothelial Cells Promote Expansion of Long-Term Engrafting Marrow Hematopoietic Stem and Progenitor Cells in Primates.

Stem cells translational medicine

Gori JL,Butler JM,Kunar B,Poulos MG,Ginsberg M,Nolan DJ,Norgaard ZK,Adair JE,Rafii S,Kiem HP

Wed Mar 01 00:00:00 EST 2017

Endothelial Cells Promote Expansion of Long-Term Engrafting Marrow Hematopoietic Stem and Progenitor Cells in Primates.

Stem cells translational medicine

Gori JL,Butler JM,Kunar B,Poulos MG,Ginsberg M,Nolan DJ,Norgaard ZK,Adair JE,Rafii S,Kiem HP

Wed Mar 01 00:00:00 EST 2017

CXCL12-CXCR4 Axis Is Required for Contact-Mediated Human B Lymphoid and Plasmacytoid Dendritic Cell Differentiation but Not T Lymphoid Generation.

Journal of immunology (Baltimore, Md. : 1950)

Minami H,Nagaharu K,Nakamori Y,Ohishi K,Shimojo N,Kageyama Y,Matsumoto T,Sugimoto Y,Tawara I,Masuya M,Miwa H,Katayama N

Sun Oct 01 00:00:00 EDT 2017

Arachidonic acid and Docosahexanoic acid enhance platelet formation from human apheresis-derived CD34⁺ cells.

Cell cycle (Georgetown, Tex.)

Dhenge A,Limbkar K,Melinkeri S,Kale VP,Limaye L

300-18 was used in Cell Culture to examine the role of poly-unsaturated fatty acids in platelet production.

Fri May 19 00:00:00 EDT 2017

The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age.

Nature communications

Hosokawa K,MacArthur BD,Ikushima YM,Toyama H,Masuhiro Y,Hanazawa S,Suda T,Arai F

Fri Oct 06 00:00:00 EDT 2017

The combination of CHK1 inhibitor with G-CSF overrides cytarabine resistance in human acute myeloid leukemia.

Nature communications

Di Tullio A,Rouault-Pierre K,Abarrategi A,Mian S,Grey W,Gribben J,Stewart A,Blackwood E,Bonnet D

Wed Nov 22 00:00:00 EST 2017

Nuclear Factor Erythroid 2 Regulates Human HSC Self-Renewal and T Cell Differentiation by Preventing NOTCH1 Activation.

Stem cell reports

Di Tullio A,Passaro D,Rouault-Pierre K,Purewal S,Bonnet D

Tue Jul 11 00:00:00 EDT 2017

Enforced GFI1 expression impedes human and murine leukemic cell growth.

Scientific reports

Hönes JM,Thivakaran A,Botezatu L,Patnana P,Castro SVDC,Al-Matary YS,Schütte J,Fischer KBI,Vassen L,Görgens A,Dührsen U,Giebel B,Khandanpour C

Thu Nov 16 00:00:00 EST 2017

Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate.

Nature biotechnology

Stevens MM,Maire CL,Chou N,Murakami MA,Knoff DS,Kikuchi Y,Kimmerling RJ,Liu H,Haidar S,Calistri NL,Cermak N,Olcum S,Cordero NA,Idbaih A,Wen PY,Weinstock DM,Ligon KL,Manalis SR

300-18 was used in In vitro experiments to investigate assays for tumour cell therapeutic response.

Tue Nov 01 00:00:00 EDT 2016

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

Thu Apr 07 00:00:00 EDT 2016

CCL5 derived from platelets increases megakaryocyte proplatelet formation.

Blood

Machlus KR,Johnson KE,Kulenthirarajan R,Forward JA,Tippy MD,Soussou TS,El-Husayni SH,Wu SK,Wang S,Watnick RS,Italiano JE,Battinelli EM

Thu Feb 18 00:00:00 EST 2016

UV-inactivated HSV-1 potently activates NK cell killing of leukemic cells.

Blood

Samudio I,Rezvani K,Shaim H,Hofs E,Ngom M,Bu L,Liu G,Lee JT,Imren S,Lam V,Poon GF,Ghaedi M,Takei F,Humphries K,Jia W,Krystal G

Thu May 26 00:00:00 EDT 2016

Lin- CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitors.

Blood

Dahlin JS,Malinovschi A,Öhrvik H,Sandelin M,Janson C,Alving K,Hallgren J

Thu Jan 28 00:00:00 EST 2016

Effect of lenalidomide treatment on clonal architecture of myelodysplastic syndromes without 5q deletion.

Blood

Chesnais V,Renneville A,Toma A,Lambert J,Passet M,Dumont F,Chevret S,Lejeune J,Raimbault A,Stamatoullas A,Rose C,Beyne-Rauzy O,Delaunay J,Solary E,Fenaux P,Dreyfus F,Preudhomme C,Kosmider O,Fontenay M

Thu Feb 11 00:00:00 EST 2016

Sorting protein VPS33B regulates exosomal autocrine signaling to mediate hematopoiesis and leukemogenesis.

The Journal of clinical investigation

Gu H,Chen C,Hao X,Wang C,Zhang X,Li Z,Shao H,Zeng H,Yu Z,Xie L,Xia F,Zhang F,Liu X,Zhang Y,Jiang H,Zhu J,Wan J,Wang C,Weng W,Xie J,Tao M,Zhang CC,Liu J,Chen GQ,Zheng J

Thu Dec 01 00:00:00 EST 2016

Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge's disease.

The Journal of experimental medicine

Azad P,Zhao HW,Cabrales PJ,Ronen R,Zhou D,Poulsen O,Appenzeller O,Hsiao YH,Bafna V,Haddad GG

Mon Nov 14 00:00:00 EST 2016

Loss of Ezh2 synergizes with JAK2-V617F in initiating myeloproliferative neoplasms and promoting myelofibrosis.

The Journal of experimental medicine

Shimizu T,Kubovcakova L,Nienhold R,Zmajkovic J,Meyer SC,Hao-Shen H,Geier F,Dirnhofer S,Guglielmelli P,Vannucchi AM,Feenstra JD,Kralovics R,Orkin SH,Skoda RC

Mon Jul 25 00:00:00 EDT 2016

Thrombopoietin/TGF-β1 Loop Regulates Megakaryocyte Extracellular Matrix Component Synthesis.

Stem cells (Dayton, Ohio)

Abbonante V,Di Buduo CA,Gruppi C,Malara A,Gianelli U,Celesti G,Anselmo A,Laghi L,Vercellino M,Visai L,Iurlo A,Moratti R,Barosi G,Rosti V,Balduini A

Fri Apr 01 00:00:00 EDT 2016

Molecular Analysis of Neutrophil Differentiation from Human Induced Pluripotent Stem Cells Delineates the Kinetics of Key Regulators of Hematopoiesis.

Stem cells (Dayton, Ohio)

Sweeney CL,Teng R,Wang H,Merling RK,Lee J,Choi U,Koontz S,Wright DG,Malech HL

Wed Jun 01 00:00:00 EDT 2016

Molecular Analysis of Neutrophil Differentiation from Human Induced Pluripotent Stem Cells Delineates the Kinetics of Key Regulators of Hematopoiesis.

Stem cells (Dayton, Ohio)

Sweeney CL,Teng R,Wang H,Merling RK,Lee J,Choi U,Koontz S,Wright DG,Malech HL

Wed Jun 01 00:00:00 EDT 2016

Letter to the Editor: Production of Mature Healthy Hematopoietic Cells from Induced Pluripotent Stem Cells Derived from an AML Diagnostic Sample Containing the t(8;21) Translocation.

Stem cells (Dayton, Ohio)

Hoffmann D,Göhring G,Heuser M,Ganser A,Schambach A,Morgan MA

Tue Mar 01 00:00:00 EST 2016

CDKIs p18(INK4c) and p57(Kip2) are involved in quiescence of CML leukemic stem cells after treatment with TKI.

Cell cycle (Georgetown, Tex.)

Moreno-Lorenzana D,Avilés-Vazquez S,Sandoval Esquivel MA,Alvarado-Moreno A,Ortiz-Navarrete V,Torres-Martínez H,Ayala-Sánchez M,Mayani H,Chavez-Gonzalez A

300-18 was used in Sample Preparation (FC/FACS) to analyze the cell cycle status, levels of several CDKIs and the subcellular localization of such molecules in different Chronic Myeloid Leukemia (CML) cell lines, as well as primary CD34(+)CD38(-)lin(-) LSC and HSC to understand the role of Cyclin Dependent Kinase Inhibitors in CML LSC permanence after tyrosine kinase inhibitor (TKI) treatment.

Mon May 02 00:00:00 EDT 2016

GSK3β Inhibition Promotes Efficient Myeloid and Lymphoid Hematopoiesis from Non-human Primate-Induced Pluripotent Stem Cells.

Stem cell reports

D'Souza SS,Maufort J,Kumar A,Zhang J,Smuga-Otto K,Thomson JA,Slukvin II

Tue Feb 09 00:00:00 EST 2016

Single-cell RNA sequencing reveals molecular and functional platelet bias of aged haematopoietic stem cells.

Nature communications

Grover A,Sanjuan-Pla A,Thongjuea S,Carrelha J,Giustacchini A,Gambardella A,Macaulay I,Mancini E,Luis TC,Mead A,Jacobsen SE,Nerlov C

Thu Mar 24 00:00:00 EDT 2016

Synthetic viability by BRCA2 and PARP1/ARTD1 deficiencies.

Nature communications

Ding X,Ray Chaudhuri A,Callen E,Pang Y,Biswas K,Klarmann KD,Martin BK,Burkett S,Cleveland L,Stauffer S,Sullivan T,Dewan A,Marks H,Tubbs AT,Wong N,Buehler E,Akagi K,Martin SE,Keller JR,Nussenzweig A,Sharan SK

Mon Aug 08 00:00:00 EDT 2016

Semi-automated closed system manufacturing of lentivirus gene-modified haematopoietic stem cells for gene therapy.

Nature communications

Adair JE,Waters T,Haworth KG,Kubek SP,Trobridge GD,Hocum JD,Heimfeld S,Kiem HP

Thu Oct 20 00:00:00 EDT 2016

A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells.

PloS one

Ahmadian Baghbaderani B,Tian X,Scotty Cadet J,Shah K,Walde A,Tran H,Kovarcik DP,Clarke D,Fellner T

300-18 was used in Cell Culture to show that the medium plays an integral role in establishing a cGMP-compliant process for the manufacturing of hiPSCs that can be used for generation of clinically relevant cell types for cell replacement therapy applications.

Fri Aug 04 00:00:00 EDT 2017

Disruption of Notch signaling aggravates irradiation-induced bone marrow injury, which is ameliorated by a soluble Dll1 ligand through Csf2rb2 upregulation.

Scientific reports

Chen JJ,Gao XT,Yang L,Fu W,Liang L,Li JC,Hu B,Sun ZJ,Huang SY,Zhang YZ,Liang YM,Qin HY,Han H

Wed May 18 00:00:00 EDT 2016

Disruption of Notch signaling aggravates irradiation-induced bone marrow injury, which is ameliorated by a soluble Dll1 ligand through Csf2rb2 upregulation.

Scientific reports

Chen JJ,Gao XT,Yang L,Fu W,Liang L,Li JC,Hu B,Sun ZJ,Huang SY,Zhang YZ,Liang YM,Qin HY,Han H

Wed May 18 00:00:00 EDT 2016

ASXL1 plays an important role in erythropoiesis.

Scientific reports

Shi H,Yamamoto S,Sheng M,Bai J,Zhang P,Chen R,Chen S,Shi L,Abdel-Wahab O,Xu M,Zhou Y,Yang FC

Wed Jun 29 00:00:00 EDT 2016

Recombinant Collagen Engineered to Bind to Discoidin Domain Receptor Functions as a Receptor Inhibitor.

The Journal of biological chemistry

An B,Abbonante V,Xu H,Gavriilidou D,Yoshizumi A,Bihan D,Farndale RW,Kaplan DL,Balduini A,Leitinger B,Brodsky B

Fri Feb 26 00:00:00 EST 2016

Chromothriptic cure of WHIM syndrome.

Cell

McDermott DH,Gao JL,Liu Q,Siwicki M,Martens C,Jacobs P,Velez D,Yim E,Bryke CR,Hsu N,Dai Z,Marquesen MM,Stregevsky E,Kwatemaa N,Theobald N,Long Priel DA,Pittaluga S,Raffeld MA,Calvo KR,Maric I,Desmond R,Holmes KL,Kuhns DB,Balabanian K,Bachelerie F,Porcella SF,Malech HL,Murphy PM

Thu Feb 12 00:00:00 EST 2015

Jarid2 regulates hematopoietic stem cell function by acting with polycomb repressive complex 2.

Blood

Kinkel SA,Galeev R,Flensburg C,Keniry A,Breslin K,Gilan O,Lee S,Liu J,Chen K,Gearing LJ,Moore DL,Alexander WS,Dawson M,Majewski IJ,Oshlack A,Larsson J,Blewitt ME

Thu Mar 19 00:00:00 EDT 2015

Correction of the sickle cell disease mutation in human hematopoietic stem/progenitor cells.

Blood

Hoban MD,Cost GJ,Mendel MC,Romero Z,Kaufman ML,Joglekar AV,Ho M,Lumaquin D,Gray D,Lill GR,Cooper AR,Urbinati F,Senadheera S,Zhu A,Liu PQ,Paschon DE,Zhang L,Rebar EJ,Wilber A,Wang X,Gregory PD,Holmes MC,Reik A,Hollis RP,Kohn DB

Thu Apr 23 00:00:00 EDT 2015

Notch1 acts via Foxc2 to promote definitive hematopoiesis via effects on hemogenic endothelium.

Blood

Jang IH,Lu YF,Zhao L,Wenzel PL,Kume T,Datta SM,Arora N,Guiu J,Lagha M,Kim PG,Do EK,Kim JH,Schlaeger TM,Zon LI,Bigas A,Burns CE,Daley GQ

Thu Feb 26 00:00:00 EST 2015

A hyperactive Mpl-based cell growth switch drives macrophage-associated erythropoiesis through an erythroid-megakaryocytic precursor.

Blood

Belay E,Miller CP,Kortum AN,Torok-Storb B,Blau CA,Emery DW

Thu Feb 05 00:00:00 EST 2015

Deletion of Stat3 in hematopoietic cells enhances thrombocytosis and shortens survival in a JAK2-V617F mouse model of MPN.

Blood

Grisouard J,Shimizu T,Duek A,Kubovcakova L,Hao-Shen H,Dirnhofer S,Skoda RC

Thu Mar 26 00:00:00 EDT 2015

MLL leukemia induction by genome editing of human CD34+ hematopoietic cells.

Blood

Buechele C,Breese EH,Schneidawind D,Lin CH,Jeong J,Duque-Afonso J,Wong SH,Smith KS,Negrin RS,Porteus M,Cleary ML

Thu Oct 01 00:00:00 EDT 2015

Specific molecular signatures predict decitabine response in chronic myelomonocytic leukemia.

The Journal of clinical investigation

Meldi K,Qin T,Buchi F,Droin N,Sotzen J,Micol JB,Selimoglu-Buet D,Masala E,Allione B,Gioia D,Poloni A,Lunghi M,Solary E,Abdel-Wahab O,Santini V,Figueroa ME

Fri May 01 00:00:00 EDT 2015

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

Mon Mar 02 00:00:00 EST 2015

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

Mon Mar 02 00:00:00 EST 2015

Glucocorticoids improve erythroid progenitor maintenance and dampen Trp53 response in a mouse model of Diamond-Blackfan anaemia.

British journal of haematology

Sjögren SE,Siva K,Soneji S,George AJ,Winkler M,Jaako P,Wlodarski M,Karlsson S,Hannan RD,Flygare J

Sun Nov 01 00:00:00 EDT 2015

Molecular signature of erythroblast enucleation in human embryonic stem cells.

Stem cells (Dayton, Ohio)

Rouzbeh S,Kobari L,Cambot M,Mazurier C,Hebert N,Faussat AM,Durand C,Douay L,Lapillonne H

Sat Aug 01 00:00:00 EDT 2015

PRC2 inhibition counteracts the culture-associated loss of engraftment potential of human cord blood-derived hematopoietic stem and progenitor cells.

Scientific reports

Varagnolo L,Lin Q,Obier N,Plass C,Dietl J,Zenke M,Claus R,Müller AM

Wed Jul 22 00:00:00 EDT 2015

Generation of a lentiviral vector producer cell clone for human Wiskott-Aldrich syndrome gene therapy.

Molecular therapy. Methods & clinical development

Wielgosz MM,Kim YS,Carney GG,Zhan J,Reddivari M,Coop T,Heath RJ,Brown SA,Nienhuis AW

300-18 was used in In vitro experiments to show that the producer cell line and the specific monoclonal antibody will facilitate the development of a clinical protocol for gene transfer into WAS protein deficient stem cells.

Mon Jun 08 00:00:00 EDT 2015

Fancd2 is required for nuclear retention of Foxo3a in hematopoietic stem cell maintenance.

The Journal of biological chemistry

Li X,Li J,Wilson A,Sipple J,Schick J,Pang Q

Fri Jan 30 00:00:00 EST 2015

Highly efficient in vitro and in vivo delivery of functional RNAs using new versatile MS2-chimeric retrovirus-like particles.

Molecular therapy. Methods & clinical development

Prel A,Caval V,Gayon R,Ravassard P,Duthoit C,Payen E,Maouche-Chretien L,Creneguy A,Nguyen TH,Martin N,Piver E,Sevrain R,Lamouroux L,Leboulch P,Deschaseaux F,Bouillé P,Sensébé L,Pagès JC

Tue Nov 03 00:00:00 EST 2015

Discovery of novel INK4C small-molecule inhibitors to promote human and murine hematopoietic stem cell ex vivo expansion.

Scientific reports

Xie XQ,Yang P,Zhang Y,Zhang P,Wang L,Ding Y,Yang M,Tong Q,Cheng H,Ji Q,McGuire T,Yuan W,Cheng T,Gao Y

Fri Dec 18 00:00:00 EST 2015

Discovery of novel INK4C small-molecule inhibitors to promote human and murine hematopoietic stem cell ex vivo expansion.

Scientific reports

Xie XQ,Yang P,Zhang Y,Zhang P,Wang L,Ding Y,Yang M,Tong Q,Cheng H,Ji Q,McGuire T,Yuan W,Cheng T,Gao Y

Fri Dec 18 00:00:00 EST 2015

Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Jomen Y,Ishihara K,Kim J,Ishimoto T,Bae EJ,Mohney RP,Stirdivant SM,Oshima H,Oshima M,Kim DW,Nakauchi H,Takihara Y,Kato Y,Ooshima A,Kim SJ

Thu Aug 20 00:00:00 EDT 2015

SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.

Nature communications

Mian SA,Rouault-Pierre K,Smith AE,Seidl T,Pizzitola I,Kizilors A,Kulasekararaj AG,Bonnet D,Mufti GJ

Tue Dec 08 00:00:00 EST 2015

Construction of stable packaging cell lines for clinical lentiviral vector production.

Scientific reports

Sanber KS,Knight SB,Stephen SL,Bailey R,Escors D,Minshull J,Santilli G,Thrasher AJ,Collins MK,Takeuchi Y

Thu Mar 12 00:00:00 EDT 2015

Targeted genome editing in human repopulating haematopoietic stem cells.

Nature

Genovese P,Schiroli G,Escobar G,Tomaso TD,Firrito C,Calabria A,Moi D,Mazzieri R,Bonini C,Holmes MC,Gregory PD,van der Burg M,Gentner B,Montini E,Lombardo A,Naldini L

Thu Jun 12 00:00:00 EDT 2014

Differentiation therapy for the treatment of t(8;21) acute myeloid leukemia using histone deacetylase inhibitors.

Blood

Bots M,Verbrugge I,Martin BP,Salmon JM,Ghisi M,Baker A,Stanley K,Shortt J,Ossenkoppele GJ,Zuber J,Rappaport AR,Atadja P,Lowe SW,Johnstone RW

Thu Feb 27 00:00:00 EST 2014

Differentiation therapy for the treatment of t(8;21) acute myeloid leukemia using histone deacetylase inhibitors.

Blood

Bots M,Verbrugge I,Martin BP,Salmon JM,Ghisi M,Baker A,Stanley K,Shortt J,Ossenkoppele GJ,Zuber J,Rappaport AR,Atadja P,Lowe SW,Johnstone RW

Thu Feb 27 00:00:00 EST 2014

Depletion of Sf3b1 impairs proliferative capacity of hematopoietic stem cells but is not sufficient to induce myelodysplasia.

Blood

Wang C,Sashida G,Saraya A,Ishiga R,Koide S,Oshima M,Isono K,Koseki H,Iwama A

Thu May 22 00:00:00 EDT 2014

The IL-2/CD25 axis maintains distinct subsets of chronic myeloid leukemia-initiating cells.

Blood

Kobayashi CI,Takubo K,Kobayashi H,Nakamura-Ishizu A,Honda H,Kataoka K,Kumano K,Akiyama H,Sudo T,Kurokawa M,Suda T

Thu Apr 17 00:00:00 EDT 2014

Rapamycin relieves lentiviral vector transduction resistance in human and mouse hematopoietic stem cells.

Blood

Wang CX,Sather BD,Wang X,Adair J,Khan I,Singh S,Lang S,Adams A,Curinga G,Kiem HP,Miao CH,Rawlings DJ,Torbett BE

Thu Aug 07 00:00:00 EDT 2014

The DPY30 subunit in SET1/MLL complexes regulates the proliferation and differentiation of hematopoietic progenitor cells.

Blood

Yang Z,Augustin J,Chang C,Hu J,Shah K,Chang CW,Townes T,Jiang H

Thu Sep 25 00:00:00 EDT 2014

CD33 target validation and sustained depletion of AML blasts in long-term cultures by the bispecific T-cell-engaging antibody AMG 330.

Blood

Krupka C,Kufer P,Kischel R,Zugmaier G,Bögeholz J,Köhnke T,Lichtenegger FS,Schneider S,Metzeler KH,Fiegl M,Spiekermann K,Baeuerle PA,Hiddemann W,Riethmüller G,Subklewe M

Thu Jan 16 00:00:00 EST 2014

Thrombocytopenia-associated mutations in the ANKRD26 regulatory region induce MAPK hyperactivation.

The Journal of clinical investigation

Bluteau D,Balduini A,Balayn N,Currao M,Nurden P,Deswarte C,Leverger G,Noris P,Perrotta S,Solary E,Vainchenker W,Debili N,Favier R,Raslova H

Sat Feb 01 00:00:00 EST 2014

RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast development.

The Journal of experimental medicine

Verma R,Su S,McCrann DJ,Green JM,Leu K,Young PR,Schatz PJ,Silva JC,Stokes MP,Wojchowski DM

Mon Aug 25 00:00:00 EDT 2014

BRAF-V600E expression in precursor versus differentiated dendritic cells defines clinically distinct LCH risk groups.

The Journal of experimental medicine

Berres ML,Lim KP,Peters T,Price J,Takizawa H,Salmon H,Idoyaga J,Ruzo A,Lupo PJ,Hicks MJ,Shih A,Simko SJ,Abhyankar H,Chakraborty R,Leboeuf M,Beltrão M,Lira SA,Heym KM,Bigley V,Collin M,Manz MG,McClain K,Merad M,Allen CE

Mon Apr 07 00:00:00 EDT 2014

JAK2V617F-positive endothelial cells contribute to clotting abnormalities in myeloproliferative neoplasms.

Proceedings of the National Academy of Sciences of the United States of America

Etheridge SL,Roh ME,Cosgrove ME,Sangkhae V,Fox NE,Chen J,López JA,Kaushansky K,Hitchcock IS

Tue Feb 11 00:00:00 EST 2014

Clonal Dominance With Retroviral Vector Insertions Near the ANGPT1 and ANGPT2 Genes in a Human Xenotransplant Mouse Model.

Molecular therapy. Nucleic acids

Haemmerle R,Phaltane R,Rothe M,Schröder S,Schambach A,Moritz T,Modlich U

Tue Oct 07 00:00:00 EDT 2014

Modeling chronic myeloid leukemia in immunodeficient mice reveals expansion of aberrant mast cells and accumulation of pre-B cells.

Blood cancer journal

Askmyr M,Ågerstam H,Lilljebjörn H,Hansen N,Karlsson C,von Palffy S,Landberg N,Högberg C,Lassen C,Rissler M,Richter J,Ehinger M,Järås M,Fioretos T

Fri Dec 12 00:00:00 EST 2014

Generation of iPSCs from genetically corrected Brca2 hypomorphic cells: implications in cell reprogramming and stem cell therapy.

Stem cells (Dayton, Ohio)

Navarro S,Moleiro V,Molina-Estevez FJ,Lozano ML,Chinchon R,Almarza E,Quintana-Bustamante O,Mostoslavsky G,Maetzig T,Galla M,Heinz N,Schiedlmeier B,Torres Y,Modlich U,Samper E,Río P,Segovia JC,Raya A,Güenechea G,Izpisua-Belmonte JC,Bueren JA

Sat Feb 01 00:00:00 EST 2014

EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.

Stem cells (Dayton, Ohio)

Zheng WW,Dong XM,Yin RH,Xu FF,Ning HM,Zhang MJ,Xu CW,Yang Y,Ding YL,Wang ZD,Zhao WB,Tang LJ,Chen H,Wang XH,Zhan YQ,Yu M,Ge CH,Li CY,Yang XM

Fri Aug 01 00:00:00 EDT 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Wed Jan 01 00:00:00 EST 2014

Serotonin enhances megakaryopoiesis and proplatelet formation via p-Erk1/2 and F-actin reorganization.

Stem cells (Dayton, Ohio)

Ye JY,Liang EY,Cheng YS,Chan GC,Ding Y,Meng F,Ng MH,Chong BH,Lian Q,Yang M

Sat Nov 01 00:00:00 EDT 2014

Serotonin enhances megakaryopoiesis and proplatelet formation via p-Erk1/2 and F-actin reorganization.

Stem cells (Dayton, Ohio)

Ye JY,Liang EY,Cheng YS,Chan GC,Ding Y,Meng F,Ng MH,Chong BH,Lian Q,Yang M

Sat Nov 01 00:00:00 EDT 2014

Serotonin enhances megakaryopoiesis and proplatelet formation via p-Erk1/2 and F-actin reorganization.

Stem cells (Dayton, Ohio)

Ye JY,Liang EY,Cheng YS,Chan GC,Ding Y,Meng F,Ng MH,Chong BH,Lian Q,Yang M

Sat Nov 01 00:00:00 EDT 2014

Conversion of human fibroblasts into monocyte-like progenitor cells.

Stem cells (Dayton, Ohio)

Pulecio J,Nivet E,Sancho-Martinez I,Vitaloni M,Guenechea G,Xia Y,Kurian L,Dubova I,Bueren J,Laricchia-Robbio L,Belmonte JCI

Sat Nov 01 00:00:00 EDT 2014

Conversion of human fibroblasts into monocyte-like progenitor cells.

Stem cells (Dayton, Ohio)

Pulecio J,Nivet E,Sancho-Martinez I,Vitaloni M,Guenechea G,Xia Y,Kurian L,Dubova I,Bueren J,Laricchia-Robbio L,Belmonte JCI

Sat Nov 01 00:00:00 EDT 2014

Pcgf6, a polycomb group protein, regulates mesodermal lineage differentiation in murine ESCs and functions in iPS reprogramming.

Stem cells (Dayton, Ohio)

Zdzieblo D,Li X,Lin Q,Zenke M,Illich DJ,Becker M,Müller AM

Mon Dec 01 00:00:00 EST 2014

Platelet-secreted microRNA-223 promotes endothelial cell apoptosis induced by advanced glycation end products via targeting the insulin-like growth factor 1 receptor.

Journal of immunology (Baltimore, Md. : 1950)

Pan Y,Liang H,Liu H,Li D,Chen X,Li L,Zhang CY,Zen K

Wed Jan 01 00:00:00 EST 2014

Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Balan S,Ollion V,Colletti N,Chelbi R,Montanana-Sanchis F,Liu H,Vu Manh TP,Sanchez C,Savoret J,Perrot I,Doffin AC,Fossum E,Bechlian D,Chabannon C,Bogen B,Asselin-Paturel C,Shaw M,Soos T,Caux C,Valladeau-Guilemond J,Dalod M

Fri Aug 15 00:00:00 EDT 2014

Aspirin influences megakaryocytic gene expression leading to up-regulation of multidrug resistance protein-4 in human platelets.

British journal of clinical pharmacology

Massimi I,Guerriero R,Lotti LV,Lulli V,Borgognone A,Romani F,Barillà F,Gaudio C,Gabbianelli M,Frati L,Pulcinelli FM

Mon Dec 01 00:00:00 EST 2014

Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system.

The Journal of biological chemistry

An B,Abbonante V,Yigit S,Balduini A,Kaplan DL,Brodsky B

Fri Feb 21 00:00:00 EST 2014

Ablation of Fbxw7 eliminates leukemia-initiating cells by preventing quiescence.

Cancer cell

Takeishi S,Matsumoto A,Onoyama I,Naka K,Hirao A,Nakayama KI

Mon Mar 18 00:00:00 EDT 2013

Loss of the F-BAR protein CIP4 reduces platelet production by impairing membrane-cytoskeleton remodeling.

Blood

Chen Y,Aardema J,Kale S,Whichard ZL,Awomolo A,Blanchard E,Chang B,Myers DR,Ju L,Tran R,Reece D,Christensen H,Boukour S,Debili N,Strom TS,Rawlings D,Vázquez FX,Voth GA,Zhu C,Kahr WH,Lam WA,Corey SJ

Thu Sep 05 00:00:00 EDT 2013

RUNX1/AML1 mutant collaborates with BMI1 overexpression in the development of human and murine myelodysplastic syndromes.

Blood

Harada Y,Inoue D,Ding Y,Imagawa J,Doki N,Matsui H,Yahata T,Matsushita H,Ando K,Sashida G,Iwama A,Kitamura T,Harada H

Thu Apr 25 00:00:00 EDT 2013

FLVCR is necessary for erythroid maturation, may contribute to platelet maturation, but is dispensable for normal hematopoietic stem cell function.

Blood

Byon JC,Chen J,Doty RT,Abkowitz JL

Thu Oct 17 00:00:00 EDT 2013

CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age.

Blood

Gekas C,Graf T

Thu May 30 00:00:00 EDT 2013

Antisense-based RNA therapy of factor V deficiency: in vitro and ex vivo rescue of a F5 deep-intronic splicing mutation.

Blood

Nuzzo F,Radu C,Baralle M,Spiezia L,Hackeng TM,Simioni P,Castoldi E

Thu Nov 28 00:00:00 EST 2013

Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34+ cells.

Blood

Merling RK,Sweeney CL,Choi U,De Ravin SS,Myers TG,Otaizo-Carrasquero F,Pan J,Linton G,Chen L,Koontz S,Theobald NL,Malech HL

Thu Apr 04 00:00:00 EDT 2013

Role of SOX17 in hematopoietic development from human embryonic stem cells.

Blood

Nakajima-Takagi Y,Osawa M,Oshima M,Takagi H,Miyagi S,Endoh M,Endo TA,Takayama N,Eto K,Toyoda T,Koseki H,Nakauchi H,Iwama A

Thu Jan 17 00:00:00 EST 2013

The human NPM1 mutation A perturbs megakaryopoiesis in a conditional mouse model.

Blood

Sportoletti P,Varasano E,Rossi R,Bereshchenko O,Cecchini D,Gionfriddo I,Bolli N,Tiacci E,Intermesoli T,Zanghì P,Masciulli A,Martelli MP,Falzetti F,Martelli MF,Falini B

Thu Apr 25 00:00:00 EDT 2013

Perturbation of fetal hematopoiesis in a mouse model of Down syndrome's transient myeloproliferative disorder.

Blood

Birger Y,Goldberg L,Chlon TM,Goldenson B,Muler I,Schiby G,Jacob-Hirsch J,Rechavi G,Crispino JD,Izraeli S

Thu Aug 08 00:00:00 EDT 2013

Perturbation of fetal hematopoiesis in a mouse model of Down syndrome's transient myeloproliferative disorder.

Blood

Birger Y,Goldberg L,Chlon TM,Goldenson B,Muler I,Schiby G,Jacob-Hirsch J,Rechavi G,Crispino JD,Izraeli S

Thu Aug 08 00:00:00 EDT 2013

Identification of the chemokine CCL28 as a growth and survival factor for human hematopoietic stem and progenitor cells.

Blood

Karlsson C,Baudet A,Miharada N,Soneji S,Gupta R,Magnusson M,Enver T,Karlsson G,Larsson J

Thu May 09 00:00:00 EDT 2013

PEAR1 attenuates megakaryopoiesis via control of the PI3K/PTEN pathway.

Blood

Kauskot A,Vandenbriele C,Louwette S,Gijsbers R,Tousseyn T,Freson K,Verhamme P,Hoylaerts MF

Thu Jun 27 00:00:00 EDT 2013

Genomic impact of transient low-dose decitabine treatment on primary AML cells.

Blood

Klco JM,Spencer DH,Lamprecht TL,Sarkaria SM,Wylie T,Magrini V,Hundal J,Walker J,Varghese N,Erdmann-Gilmore P,Lichti CF,Meyer MR,Townsend RR,Wilson RK,Mardis ER,Ley TJ

Thu Feb 28 00:00:00 EST 2013

Cooperativity of imprinted genes inactivated by acquired chromosome 20q deletions.

The Journal of clinical investigation

Aziz A,Baxter EJ,Edwards C,Cheong CY,Ito M,Bench A,Kelley R,Silber Y,Beer PA,Chng K,Renfree MB,McEwen K,Gray D,Nangalia J,Mufti GJ,Hellstrom-Lindberg E,Kiladjian JJ,McMullin MF,Campbell PJ,Ferguson-Smith AC,Green AR

Wed May 01 00:00:00 EDT 2013

Isocitrate ameliorates anemia by suppressing the erythroid iron restriction response.

The Journal of clinical investigation

Richardson CL,Delehanty LL,Bullock GC,Rival CM,Tung KS,Kimpel DL,Gardenghi S,Rivella S,Goldfarb AN

Thu Aug 01 00:00:00 EDT 2013

Sirt1 ablation promotes stress-induced loss of epigenetic and genomic hematopoietic stem and progenitor cell maintenance.

The Journal of experimental medicine

Singh SK,Williams CA,Klarmann K,Burkett SS,Keller JR,Oberdoerffer P

Mon May 06 00:00:00 EDT 2013

Notch pathway activation targets AML-initiating cell homeostasis and differentiation.

The Journal of experimental medicine

Lobry C,Ntziachristos P,Ndiaye-Lobry D,Oh P,Cimmino L,Zhu N,Araldi E,Hu W,Freund J,Abdel-Wahab O,Ibrahim S,Skokos D,Armstrong SA,Levine RL,Park CY,Aifantis I

Mon Feb 11 00:00:00 EST 2013

Preclinical safety and efficacy of human CD34(+) cells transduced with lentiviral vector for the treatment of Wiskott-Aldrich syndrome.

Molecular therapy : the journal of the American Society of Gene Therapy

Scaramuzza S,Biasco L,Ripamonti A,Castiello MC,Loperfido M,Draghici E,Hernandez RJ,Benedicenti F,Radrizzani M,Salomoni M,Ranzani M,Bartholomae CC,Vicenzi E,Finocchi A,Bredius R,Bosticardo M,Schmidt M,von Kalle C,Montini E,Biffi A,Roncarolo MG,Naldini L,Villa A,Aiuti A

Tue Jan 01 00:00:00 EST 2013

Preclinical in vivo evaluation of the safety of a multi-shRNA-based gene therapy against HIV-1.

Molecular therapy. Nucleic acids

Centlivre M,Legrand N,Klamer S,Liu YP,Jasmijn von Eije K,Bohne M,Rijnstra ES,Weijer K,Blom B,Voermans C,Spits H,Berkhout B

Tue Sep 03 00:00:00 EDT 2013

High doses of romiplostim induce proliferation and reduce proplatelet formation by human megakaryocytes.

PloS one

Currao M,Balduini CL,Balduini A

Fri Sep 06 00:00:00 EDT 2013

Generation of megakaryocytic progenitors from human embryonic stem cells in a feeder- and serum-free medium.

PloS one

Pick M,Azzola L,Osborne E,Stanley EG,Elefanty AG

Thu Aug 08 00:00:00 EDT 2013

Hematopoietic stem and progenitor cells acquire distinct DNA-hypermethylation during in vitro culture.

Scientific reports

Weidner CI,Walenda T,Lin Q,Wölfler MM,Denecke B,Costa IG,Zenke M,Wagner W

Thu Nov 28 00:00:00 EST 2013

Expansion on stromal cells preserves the undifferentiated state of human hematopoietic stem cells despite compromised reconstitution ability.

PloS one

Magnusson M,Sierra MI,Sasidharan R,Prashad SL,Romero M,Saarikoski P,Van Handel B,Huang A,Li X,Mikkola HK

Wed Jul 03 00:00:00 EDT 2013

Expansion on stromal cells preserves the undifferentiated state of human hematopoietic stem cells despite compromised reconstitution ability.

PloS one

Magnusson M,Sierra MI,Sasidharan R,Prashad SL,Romero M,Saarikoski P,Van Handel B,Huang A,Li X,Mikkola HK

Wed Jul 03 00:00:00 EDT 2013

Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.

Nature methods

Certo MT,Gwiazda KS,Kuhar R,Sather B,Curinga G,Mandt T,Brault M,Lambert AR,Baxter SK,Jacoby K,Ryu BY,Kiem HP,Gouble A,Paques F,Rawlings DJ,Scharenberg AM

Mon Oct 01 00:00:00 EDT 2012

FANCL ubiquitinates β-catenin and enhances its nuclear function.

Blood

Dao KH,Rotelli MD,Petersen CL,Kaech S,Nelson WD,Yates JE,Hanlon Newell AE,Olson SB,Druker BJ,Bagby GC

Thu Jul 12 00:00:00 EDT 2012

Impaired ribosomal subunit association in Shwachman-Diamond syndrome.

Blood

Burwick N,Coats SA,Nakamura T,Shimamura A

Thu Dec 20 00:00:00 EST 2012

GPIbα regulates platelet size by controlling the subcellular localization of filamin.

Blood

Kanaji T,Ware J,Okamura T,Newman PJ

Thu Mar 22 00:00:00 EDT 2012

RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Blood

Baudet A,Karlsson C,Safaee Talkhoncheh M,Galeev R,Magnusson M,Larsson J

Thu Jun 28 00:00:00 EDT 2012

Efficient generation, purification, and expansion of CD34(+) hematopoietic progenitor cells from nonhuman primate-induced pluripotent stem cells.

Blood

Gori JL,Chandrasekaran D,Kowalski JP,Adair JE,Beard BC,D'Souza SL,Kiem HP

Thu Sep 27 00:00:00 EDT 2012

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

Thu Dec 13 00:00:00 EST 2012

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

Thu Dec 13 00:00:00 EST 2012

Whole transcriptome characterization of aberrant splicing events induced by lentiviral vector integrations.

The Journal of clinical investigation

Cesana D,Sgualdino J,Rudilosso L,Merella S,Naldini L,Montini E

Tue May 01 00:00:00 EDT 2012

mTORC1 is essential for leukemia propagation but not stem cell self-renewal.

The Journal of clinical investigation

Hoshii T,Tadokoro Y,Naka K,Ooshio T,Muraguchi T,Sugiyama N,Soga T,Araki K,Yamamura K,Hirao A

Fri Jun 01 00:00:00 EDT 2012

A novel murine model of myeloproliferative disorders generated by overexpression of the transcription factor NF-E2.

The Journal of experimental medicine

Kaufmann KB,Gründer A,Hadlich T,Wehrle J,Gothwal M,Bogeska R,Seeger TS,Kayser S,Pham KB,Jutzi JS,Ganzenmüller L,Steinemann D,Schlegelberger B,Wagner JM,Jung M,Will B,Steidl U,Aumann K,Werner M,Günther T,Schüle R,Rambaldi A,Pahl HL

Mon Jan 16 00:00:00 EST 2012

Characterization of novel genomic alterations and therapeutic approaches using acute megakaryoblastic leukemia xenograft models.

The Journal of experimental medicine

Thiollier C,Lopez CK,Gerby B,Ignacimouttou C,Poglio S,Duffourd Y,Guégan J,Rivera-Munoz P,Bluteau O,Mabialah V,Diop M,Wen Q,Petit A,Bauchet AL,Reinhardt D,Bornhauser B,Gautheret D,Lecluse Y,Landman-Parker J,Radford I,Vainchenker W,Dastugue N,de Botton S,Dessen P,Bourquin JP,Crispino JD,Ballerini P,Bernard OA,Pflumio F,Mercher T

Mon Oct 22 00:00:00 EDT 2012

Lethal myelofibrosis induced by Bmi1-deficient hematopoietic cells unveils a tumor suppressor function of the polycomb group genes.

The Journal of experimental medicine

Oguro H,Yuan J,Tanaka S,Miyagi S,Mochizuki-Kashio M,Ichikawa H,Yamazaki S,Koseki H,Nakauchi H,Iwama A

Mon Mar 12 00:00:00 EDT 2012

Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis.

The Journal of experimental medicine

Bauer T,Zagórska A,Jurkin J,Yasmin N,Köffel R,Richter S,Gesslbauer B,Lemke G,Strobl H

Mon Oct 22 00:00:00 EDT 2012

Perturbation of fetal liver hematopoietic stem and progenitor cell development by trisomy 21.

Proceedings of the National Academy of Sciences of the United States of America

Roy A,Cowan G,Mead AJ,Filippi S,Bohn G,Chaidos A,Tunstall O,Chan JK,Choolani M,Bennett P,Kumar S,Atkinson D,Wyatt-Ashmead J,Hu M,Stumpf MP,Goudevenou K,O'Connor D,Chou ST,Weiss MJ,Karadimitris A,Jacobsen SE,Vyas P,Roberts I

Tue Oct 23 00:00:00 EDT 2012

Intercellular cytosolic transfer correlates with mesenchymal stromal cell rescue of umbilical cord blood cell viability during ex vivo expansion.

Cytotherapy

Chu PP,Bari S,Fan X,Gay FP,Ang JM,Chiu GN,Lim SK,Hwang WY

Mon Oct 01 00:00:00 EDT 2012

Human T-lymphoid progenitors generated in a feeder-cell-free Delta-like-4 culture system promote T-cell reconstitution in NOD/SCID/γc(-/-) mice.

Stem cells (Dayton, Ohio)

Reimann C,Six E,Dal-Cortivo L,Schiavo A,Appourchaux K,Lagresle-Peyrou C,de Chappedelaine C,Ternaux B,Coulombel L,Beldjord K,Cavazzana-Calvo M,Andre-Schmutz I

Wed Aug 01 00:00:00 EDT 2012

GITR ligand provided by thrombopoietic cells inhibits NK cell antitumor activity.

Journal of immunology (Baltimore, Md. : 1950)

Placke T,Salih HR,Kopp HG

Sun Jul 01 00:00:00 EDT 2012

Generation of virus-free induced pluripotent stem cell clones on a synthetic matrix via a single cell subcloning in the naïve state.

PloS one

Nishishita N,Shikamura M,Takenaka C,Takada N,Fusaki N,Kawamata S

Thu Dec 13 00:00:00 EST 2012

Human umbilical cord blood-derived CD34+ cells reverse osteoporosis in NOD/SCID mice by altering osteoblastic and osteoclastic activities.

PloS one

Aggarwal R,Lu J,Kanji S,Joseph M,Das M,Noble GJ,McMichael BK,Agarwal S,Hart RT,Sun Z,Lee BS,Rosol TJ,Jackson R,Mao HQ,Pompili VJ,Das H

Wed Dec 12 00:00:00 EST 2012

Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.

PloS one

Danova-Alt R,Heider A,Egger D,Cross M,Alt R

Tue Aug 07 00:00:00 EDT 2012

Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.

PloS one

Pelosi E,Castelli G,Martin-Padura I,Bordoni V,Santoro S,Conigliaro A,Cerio AM,De Santis Puzzonia M,Marighetti P,Biffoni M,Alonzi T,Amicone L,Alcalay M,Bertolini F,Testa U,Tripodi M

Tue May 21 00:00:00 EDT 2013

Bmi1 confers resistance to oxidative stress on hematopoietic stem cells.

PloS one

Nakamura S,Oshima M,Yuan J,Saraya A,Miyagi S,Konuma T,Yamazaki S,Osawa M,Nakauchi H,Koseki H,Iwama A

Tue Oct 16 00:00:00 EDT 2012

Pharmacological inhibition of caspase and calpain proteases: a novel strategy to enhance the homing responses of cord blood HSPCs during expansion.

PloS one

Sangeetha VM,Kadekar D,Kale VP,Limaye LS

Mon May 14 00:00:00 EDT 2012

Suppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells.

The Journal of biological chemistry

Gao H,Wu X,Sun Y,Zhou S,Silberstein LE,Zhu Z

Fri Aug 24 00:00:00 EDT 2012

Itga2b regulation at the onset of definitive hematopoiesis and commitment to differentiation.

PloS one

Dumon S,Walton DS,Volpe G,Wilson N,Dassé E,Del Pozzo W,Landry JR,Turner B,O'Neill LP,Göttgens B,Frampton J

Tue Feb 19 00:00:00 EST 2013

Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications.

PloS one

Yamagata Y,Parietti V,Stockholm D,Corre G,Poinsignon C,Touleimat N,Delafoy D,Besse C,Tost J,Galy A,Paldi A

Wed Jul 24 00:00:00 EDT 2013

Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming.

PloS one

Yamaguchi T,Hamanaka S,Kamiya A,Okabe M,Kawarai M,Wakiyama Y,Umino A,Hayama T,Sato H,Lee YS,Kato-Itoh M,Masaki H,Kobayashi T,Yamazaki S,Nakauchi H

Wed Jan 16 00:00:00 EST 2013

Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming.

PloS one

Yamaguchi T,Hamanaka S,Kamiya A,Okabe M,Kawarai M,Wakiyama Y,Umino A,Hayama T,Sato H,Lee YS,Kato-Itoh M,Masaki H,Kobayashi T,Yamazaki S,Nakauchi H

Wed Jan 16 00:00:00 EST 2013

Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice.

Cell research

Lu SJ,Li F,Yin H,Feng Q,Kimbrel EA,Hahm E,Thon JN,Wang W,Italiano JE,Cho J,Lanza R

Tue Mar 01 00:00:00 EST 2011

Coexistence of LMPP-like and GMP-like leukemia stem cells in acute myeloid leukemia.

Cancer cell

Goardon N,Marchi E,Atzberger A,Quek L,Schuh A,Soneji S,Woll P,Mead A,Alford KA,Rout R,Chaudhury S,Gilkes A,Knapper S,Beldjord K,Begum S,Rose S,Geddes N,Griffiths M,Standen G,Sternberg A,Cavenagh J,Hunter H,Bowen D,Killick S,Robinson L,Price A,Macintyre E,Virgo P,Burnett A,Craddock C,Enver T,Jacobsen SE,Porcher C,Vyas P

Tue Jan 18 00:00:00 EST 2011

Rho kinase regulates the survival and transformation of cells bearing oncogenic forms of KIT, FLT3, and BCR-ABL.

Cancer cell

Mali RS,Ramdas B,Ma P,Shi J,Munugalavadla V,Sims E,Wei L,Vemula S,Nabinger SC,Goodwin CB,Chan RJ,Traina F,Visconte V,Tiu RV,Lewis TA,Stern AM,Wen Q,Crispino JD,Boswell HS,Kapur R

Tue Sep 13 00:00:00 EDT 2011

JAK2V617F-mediated phosphorylation of PRMT5 downregulates its methyltransferase activity and promotes myeloproliferation.

Cancer cell

Liu F,Zhao X,Perna F,Wang L,Koppikar P,Abdel-Wahab O,Harr MW,Levine RL,Xu H,Tefferi A,Deblasio A,Hatlen M,Menendez S,Nimer SD

Tue Feb 15 00:00:00 EST 2011

Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments.

Blood

Gibbs KD,Gilbert PM,Sachs K,Zhao F,Blau HM,Weissman IL,Nolan GP,Majeti R

Thu Apr 21 00:00:00 EDT 2011

Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice.

Blood

Chen TH,Kambal A,Krysiak K,Walshauser MA,Raju G,Tibbitts JF,Walter MJ

Thu Feb 03 00:00:00 EST 2011

Mice with ribosomal protein S19 deficiency develop bone marrow failure and symptoms like patients with Diamond-Blackfan anemia.

Blood

Jaako P,Flygare J,Olsson K,Quere R,Ehinger M,Henson A,Ellis S,Schambach A,Baum C,Richter J,Larsson J,Bryder D,Karlsson S

Thu Dec 01 00:00:00 EST 2011

Impact of gene dosage, loss of wild-type allele, and FLT3 ligand on Flt3-ITD-induced myeloproliferation.

Blood

Kharazi S,Mead AJ,Mansour A,Hultquist A,Böiers C,Luc S,Buza-Vidas N,Ma Z,Ferry H,Atkinson D,Reckzeh K,Masson K,Cammenga J,Rönnstrand L,Arai F,Suda T,Nerlov C,Sitnicka E,Jacobsen SE

Thu Sep 29 00:00:00 EDT 2011

Cytoskeletal remodeling mediated by WASp in dendritic cells is necessary for normal immune synapse formation and T-cell priming.

Blood

Bouma G,Mendoza-Naranjo A,Blundell MP,de Falco E,Parsley KL,Burns SO,Thrasher AJ

Thu Sep 01 00:00:00 EDT 2011

Dendritic cells with lymphocyte-stimulating activity differentiate from human CD133 positive precursors.

Blood

Bonetti MI,Pieri L,Domenici L,Urbani S,Romano G,Aldinucci A,Ballerini C,Monici M,Saccardi R,Basile V,Bosi A,Romagnoli P

Thu Apr 14 00:00:00 EDT 2011

Sox6 enhances erythroid differentiation in human erythroid progenitors.

Blood

Cantù C,Ierardi R,Alborelli I,Fugazza C,Cassinelli L,Piconese S,Bosè F,Ottolenghi S,Ferrari G,Ronchi A

Thu Mar 31 00:00:00 EDT 2011

Production of the plasma-cell survival factor a proliferation-inducing ligand (APRIL) peaks in myeloid precursor cells from human bone marrow.

Blood

Matthes T,Dunand-Sauthier I,Santiago-Raber ML,Krause KH,Donze O,Passweg J,McKee T,Huard B

Thu Aug 18 00:00:00 EDT 2011

Human pluripotent stem cells differentiated in fully defined medium generate hematopoietic CD34- and CD34+ progenitors with distinct characteristics.

PloS one

Chicha L,Feki A,Boni A,Irion O,Hovatta O,Jaconi M

Fri Feb 25 00:00:00 EST 2011

Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors.

PloS one

Mack AA,Kroboth S,Rajesh D,Wang WB

Mon Apr 02 00:00:00 EDT 2012

Hypoxia selects bortezomib-resistant stem cells of chronic myeloid leukemia.

PloS one

Tanturli M,Giuntoli S,Barbetti V,Rovida E,Dello Sbarba P

Fri Feb 11 00:00:00 EST 2011

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

Mon Jun 06 00:00:00 EDT 2011

Multiple ETS family proteins regulate PF4 gene expression by binding to the same ETS binding site.

PloS one

Okada Y,Nobori H,Shimizu M,Watanabe M,Yonekura M,Nakai T,Kamikawa Y,Wakimura A,Funahashi N,Naruse H,Watanabe A,Yamasaki D,Fukada S,Yasui K,Matsumoto K,Sato T,Kitajima K,Nakano T,Aird WC,Doi T

Tue Feb 28 00:00:00 EST 2012

Amino-terminal enhancer of split (AES) interacts with the oncoprotein NUP98-HOXA9 and enhances its transforming ability.

The Journal of biological chemistry

Sarma NJ,Yaseen NR

Fri Nov 11 00:00:00 EST 2011

Efficient feeder-free episomal reprogramming with small molecules.

PloS one

Yu J,Chau KF,Vodyanik MA,Jiang J,Jiang Y

Tue Mar 01 00:00:00 EST 2011

Magnetic resonance imaging of bone marrow cell-mediated interleukin-10 gene therapy of atherosclerosis.

PloS one

Sun J,Li X,Feng H,Gu H,Blair T,Li J,Soriano S,Meng Y,Zhang F,Feng Q,Yang X

Tue Feb 14 00:00:00 EST 2012

In vitro megakaryocyte differentiation and proplatelet formation in Ph-negative classical myeloproliferative neoplasms: distinct patterns in the different clinical phenotypes.

PloS one

Balduini A,Badalucco S,Pugliano MT,Baev D,De Silvestri A,Cattaneo M,Rosti V,Barosi G

Mon Nov 07 00:00:00 EST 2011

Ex vivo expansion of human hematopoietic stem cells by garcinol, a potent inhibitor of histone acetyltransferase.

PloS one

Nishino T,Wang C,Mochizuki-Kashio M,Osawa M,Nakauchi H,Iwama A

Mon Jan 30 00:00:00 EST 2012

TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia.

Nature

Naka K,Hoshii T,Muraguchi T,Tadokoro Y,Ooshio T,Kondo Y,Nakao S,Motoyama N,Hirao A

Thu Feb 04 00:00:00 EST 2010

Specific gene transfer to neurons, endothelial cells and hematopoietic progenitors with lentiviral vectors.

Nature methods

Anliker B,Abel T,Kneissl S,Hlavaty J,Caputi A,Brynza J,Schneider IC,Münch RC,Petznek H,Kontermann RE,Koehl U,Johnston IC,Keinänen K,Müller UC,Hohenadl C,Monyer H,Cichutek K,Buchholz CJ

Mon Nov 01 00:00:00 EDT 2010

β-Catenin mediates the establishment and drug resistance of MLL leukemic stem cells.

Cancer cell

Yeung J,Esposito MT,Gandillet A,Zeisig BB,Griessinger E,Bonnet D,So CW

Tue Dec 14 00:00:00 EST 2010

The first trimester human placenta is a site for terminal maturation of primitive erythroid cells.

Blood

Van Handel B,Prashad SL,Hassanzadeh-Kiabi N,Huang A,Magnusson M,Atanassova B,Chen A,Hamalainen EI,Mikkola HK

Thu Oct 28 00:00:00 EDT 2010

W41/W41 blastocyst complementation: a system for genetic modeling of hematopoiesis.

Blood

Jansson L,Larsson J

Thu Jan 07 00:00:00 EST 2010

Modeling the human 8p11-myeloproliferative syndrome in immunodeficient mice.

Blood

Agerstam H,Järås M,Andersson A,Johnels P,Hansen N,Lassen C,Rissler M,Gisselsson D,Olofsson T,Richter J,Fan X,Ehinger M,Fioretos T

Thu Sep 23 00:00:00 EDT 2010

Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development.

Blood

Böiers C,Buza-Vidas N,Jensen CT,Pronk CJ,Kharazi S,Wittmann L,Sitnicka E,Hultquist A,Jacobsen SE

Thu Jun 17 00:00:00 EDT 2010

Iron control of erythroid development by a novel aconitase-associated regulatory pathway.

Blood

Bullock GC,Delehanty LL,Talbot AL,Gonias SL,Tong WH,Rouault TA,Dewar B,Macdonald JM,Chruma JJ,Goldfarb AN

Thu Jul 08 00:00:00 EDT 2010

Serum response factor is an essential transcription factor in megakaryocytic maturation.

Blood

Halene S,Gao Y,Hahn K,Massaro S,Italiano JE,Schulz V,Lin S,Kupfer GM,Krause DS

Thu Sep 16 00:00:00 EDT 2010

Depletion of L3MBTL1 promotes the erythroid differentiation of human hematopoietic progenitor cells: possible role in 20q- polycythemia vera.

Blood

Perna F,Gurvich N,Hoya-Arias R,Abdel-Wahab O,Levine RL,Asai T,Voza F,Menendez S,Wang L,Liu F,Zhao X,Nimer SD

Thu Oct 14 00:00:00 EDT 2010

Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment.

The Journal of experimental medicine

Morita Y,Ema H,Nakauchi H

Mon Jun 07 00:00:00 EDT 2010

SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis.

The Journal of experimental medicine

Yu L,Ji W,Zhang H,Renda MJ,He Y,Lin S,Cheng EC,Chen H,Krause DS,Min W

Mon Jun 07 00:00:00 EDT 2010

Junín virus infection of human hematopoietic progenitors impairs in vitro proplatelet formation and platelet release via a bystander effect involving type I IFN signaling.

PLoS pathogens

Pozner RG,Ure AE,Jaquenod de Giusti C,D'Atri LP,Italiano JE,Torres O,Romanowski V,Schattner M,Gómez RM

Thu Apr 15 00:00:00 EDT 2010

Thrombopoietic-mesenchymal interaction that may facilitate both endochondral ossification and platelet maturation via CCN2.

Journal of cell communication and signaling

Sumiyoshi K,Kubota S,Furuta RA,Yasui K,Aoyama E,Kawaki H,Kawata K,Ohgawara T,Yamashiro T,Takigawa M

Mon Mar 01 00:00:00 EST 2010

The retinoid signaling pathway inhibits hematopoiesis and uncouples from the Hox genes during hematopoietic development.

Stem cells (Dayton, Ohio)

Szatmari I,Iacovino M,Kyba M

Wed Sep 01 00:00:00 EDT 2010

CXCR7 protein is not expressed on human or mouse leukocytes.

Journal of immunology (Baltimore, Md. : 1950)

Berahovich RD,Zabel BA,Penfold ME,Lewén S,Wang Y,Miao Z,Gan L,Pereda J,Dias J,Slukvin II,McGrath KE,Jaen JC,Schall TJ

Mon Nov 01 00:00:00 EDT 2010

CXCR7 protein is not expressed on human or mouse leukocytes.

Journal of immunology (Baltimore, Md. : 1950)

Berahovich RD,Zabel BA,Penfold ME,Lewén S,Wang Y,Miao Z,Gan L,Pereda J,Dias J,Slukvin II,McGrath KE,Jaen JC,Schall TJ

Mon Nov 01 00:00:00 EDT 2010

miR-146a is differentially expressed by myeloid dendritic cell subsets and desensitizes cells to TLR2-dependent activation.

Journal of immunology (Baltimore, Md. : 1950)

Jurkin J,Schichl YM,Koeffel R,Bauer T,Richter S,Konradi S,Gesslbauer B,Strobl H

Sat May 01 00:00:00 EDT 2010

Autoregulatory lentiviral vectors allow multiple cycles of doxycycline-inducible gene expression in human hematopoietic cells in vivo.

Gene therapy

Centlivre M,Zhou X,Pouw SM,Weijer K,Kleibeuker W,Das AT,Blom B,Seppen J,Berkhout B,Legrand N

Fri Jan 01 00:00:00 EST 2010

Protein-arginine methyltransferase 1 suppresses megakaryocytic differentiation via modulation of the p38 MAPK pathway in K562 cells.

The Journal of biological chemistry

Chang YI,Hua WK,Yao CL,Hwang SM,Hung YC,Kuan CJ,Leou JS,Lin WJ

Fri Jul 02 00:00:00 EDT 2010

Expansion of cord blood CD34 cells in presence of zVADfmk and zLLYfmk improved their in vitro functionality and in vivo engraftment in NOD/SCID mouse.

PloS one

V M S,Kale VP,Limaye LS

Tue Aug 17 00:00:00 EDT 2010

Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2.

Nature protocols

Giorgetti A,Montserrat N,Rodriguez-Piza I,Azqueta C,Veiga A,Izpisúa Belmonte JC

Thu Apr 01 00:00:00 EDT 2010

In vitro transformation of primary human CD34+ cells by AML fusion oncogenes: early gene expression profiling reveals possible drug target in AML.

PloS one

Abdul-Nabi AM,Yassin ER,Varghese N,Deshmukh H,Yaseen NR

Fri Aug 27 00:00:00 EDT 2010

Hematopoietic differentiation: a coordinated dynamical process towards attractor stable states.

BMC systems biology

Felli N,Cianetti L,Pelosi E,Carè A,Liu CG,Calin GA,Rossi S,Peschle C,Marziali G,Giuliani A

Wed Jun 16 00:00:00 EDT 2010

ErythRED, a hESC line enabling identification of erythroid cells.

Nature methods

Hatzistavrou T,Micallef SJ,Ng ES,Vadolas J,Stanley EG,Elefanty AG

Tue Sep 01 00:00:00 EDT 2009

The F12-Vif derivative Chim3 inhibits HIV-1 replication in CD4+ T lymphocytes and CD34+-derived macrophages by blocking HIV-1 DNA integration.

Blood

Porcellini S,Alberici L,Gubinelli F,Lupo R,Olgiati C,Rizzardi GP,Bovolenta C

Thu Apr 09 00:00:00 EDT 2009

Effect of the nonpeptide thrombopoietin receptor agonist Eltrombopag on bone marrow cells from patients with acute myeloid leukemia and myelodysplastic syndrome.

Blood

Will B,Kawahara M,Luciano JP,Bruns I,Parekh S,Erickson-Miller CL,Aivado MA,Verma A,Steidl U

Thu Oct 29 00:00:00 EDT 2009

AML1/RUNX1 point mutation possibly promotes leukemic transformation in myeloproliferative neoplasms.

Blood

Ding Y,Harada Y,Imagawa J,Kimura A,Harada H

Thu Dec 10 00:00:00 EST 2009

Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.

Blood

McKinney-Freeman SL,Naveiras O,Yates F,Loewer S,Philitas M,Curran M,Park PJ,Daley GQ

Thu Jul 09 00:00:00 EDT 2009

Role for MKL1 in megakaryocytic maturation.

Blood

Cheng EC,Luo Q,Bruscia EM,Renda MJ,Troy JA,Massaro SA,Tuck D,Schulz V,Mane SM,Berliner N,Sun Y,Morris SW,Qiu C,Krause DS

Thu Mar 19 00:00:00 EDT 2009

Increased expression of urokinase plasminogen activator in Quebec platelet disorder is linked to megakaryocyte differentiation.

Blood

Veljkovic DK,Rivard GE,Diamandis M,Blavignac J,Cramer-Bordé EM,Hayward CP

Thu Feb 12 00:00:00 EST 2009

Reciprocal role of GATA-1 and vitamin D receptor in human myeloid dendritic cell differentiation.

Blood

Göbel F,Taschner S,Jurkin J,Konradi S,Vaculik C,Richter S,Kneidinger D,Mühlbacher C,Bieglmayer C,Elbe-Bürger A,Strobl H

Thu Oct 29 00:00:00 EDT 2009

Undifferentiated hematopoietic cells are characterized by a genome-wide undermethylation dip around the transcription start site and a hierarchical epigenetic plasticity.

Blood

Chung YS,Kim HJ,Kim TM,Hong SH,Kwon KR,An S,Park JH,Lee S,Oh IH

Thu Dec 03 00:00:00 EST 2009

Chelation of intracellular iron with the antifungal agent ciclopirox olamine induces cell death in leukemia and myeloma cells.

Blood

Eberhard Y,McDermott SP,Wang X,Gronda M,Venugopal A,Wood TE,Hurren R,Datti A,Batey RA,Wrana J,Antholine WE,Dick JE,Schimmer AD

Thu Oct 01 00:00:00 EDT 2009

The TEL-AML1 leukemia fusion gene dysregulates the TGF-beta pathway in early B lineage progenitor cells.

The Journal of clinical investigation

Ford AM,Palmi C,Bueno C,Hong D,Cardus P,Knight D,Cazzaniga G,Enver T,Greaves M

Wed Apr 01 00:00:00 EDT 2009

RNAi screen for rapid therapeutic target identification in leukemia patients.

Proceedings of the National Academy of Sciences of the United States of America

Tyner JW,Deininger MW,Loriaux MM,Chang BH,Gotlib JR,Willis SG,Erickson H,Kovacsovics T,O'Hare T,Heinrich MC,Druker BJ

Tue May 26 00:00:00 EDT 2009

Tumor immunotherapy using gene-modified human mesenchymal stem cells loaded into synthetic extracellular matrix scaffolds.

Stem cells (Dayton, Ohio)

Compte M,Cuesta AM,Sánchez-Martín D,Alonso-Camino V,Vicario JL,Sanz L,Alvarez-Vallina L

Sun Mar 01 00:00:00 EST 2009

Aryl hydrocarbon receptor activation inhibits in vitro differentiation of human monocytes and Langerhans dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Platzer B,Richter S,Kneidinger D,Waltenberger D,Woisetschläger M,Strobl H

Wed Jul 01 00:00:00 EDT 2009

IL-7 enhances thymic human T cell development in "human immune system" Rag2-/-IL-2Rgammac-/- mice without affecting peripheral T cell homeostasis.

Journal of immunology (Baltimore, Md. : 1950)

van Lent AU,Dontje W,Nagasawa M,Siamari R,Bakker AQ,Pouw SM,Maijoor KA,Weijer K,Cornelissen JJ,Blom B,Di Santo JP,Spits H,Legrand N

Tue Dec 15 00:00:00 EST 2009

Kinetics and effect of integrin expression on human CD34(+) cells during murine leukemia virus-derived retroviral transduction with recombinant fibronectin for stem cell gene therapy.

Human gene therapy

Horiuchi Y,Onodera M,Miyagawa Y,Sato B,Onda K,Katagiri YU,Okita H,Okada M,Otsu M,Kume A,Okuyama T,Fujimoto J,Kuratsuji T,Kiyokawa N

Wed Jul 01 00:00:00 EDT 2009

Evaluation of safety and efficacy of RNAi against HIV-1 in the human immune system (Rag-2(-/-)gammac(-/-)) mouse model.

Gene therapy

ter Brake O,Legrand N,von Eije KJ,Centlivre M,Spits H,Weijer K,Blom B,Berkhout B

Thu Jan 01 00:00:00 EST 2009

Stem cell therapy with overexpressed VEGF and PDGF genes improves cardiac function in a rat infarct model.

PloS one

Das H,George JC,Joseph M,Das M,Abdulhameed N,Blitz A,Khan M,Sakthivel R,Mao HQ,Hoit BD,Kuppusamy P,Pompili VJ

Wed Oct 07 00:00:00 EDT 2009

Interleukin-27 directly induces differentiation in hematopoietic stem cells.

Blood

Seita J,Asakawa M,Ooehara J,Takayanagi S,Morita Y,Watanabe N,Fujita K,Kudo M,Mizuguchi J,Ema H,Nakauchi H,Yoshimoto T

Fri Feb 15 00:00:00 EST 2008

Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors.

Blood

Takayama N,Nishikii H,Usui J,Tsukui H,Sawaguchi A,Hiroyama T,Eto K,Nakauchi H

Sun Jun 01 00:00:00 EDT 2008

Differentiation of human embryonic stem cells into immunostimulatory dendritic cells under feeder-free culture conditions.

Clinical cancer research : an official journal of the American Association for Cancer Research

Su Z,Frye C,Bae KM,Kelley V,Vieweg J

Wed Oct 01 00:00:00 EDT 2008

TNF receptor-associated factor 6-dependent CD40 signaling primes macrophages to acquire antimicrobial activity in response to TNF-alpha.

Journal of immunology (Baltimore, Md. : 1950)

Andrade RM,Wessendarp M,Portillo JA,Yang JQ,Gomez FJ,Durbin JE,Bishop GA,Subauste CS

Tue Nov 01 00:00:00 EST 2005

Are interleukin-16 and thrombopoietin new tools for the in vitro generation of dendritic cells?

Blood

Della Bella S,Nicola S,Timofeeva I,Villa ML,Santoro A,Berardi AC

Wed Dec 15 00:00:00 EST 2004

Thrombopoietin promotes mixed lineage and megakaryocytic colony-forming cell growth but inhibits primitive and definitive erythropoiesis in cells isolated from early murine yolk sacs.

Blood

Xie X,Chan RJ,Johnson SA,Starr M,McCarthy J,Kapur R,Yoder MC

Sat Feb 15 00:00:00 EST 2003

Bioinformatics

Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; ML; MPL ligand; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; Thrombopoietin

Human TPO (Thrombopoietin) Recombinant Protein, PeproTech®

Human TPO (Thrombopoietin) Protein (300-18-10UG) in Functional

Product Details

300-18-10UG

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

We're here to help

Your items have has been added!