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Gibco
Human TPO (Thrombopoietin) Recombinant Protein
Product Details
PHC9511
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Published species
Human
Expression System
HEK293 cells
Molecular weight
85 kDa
Class
Recombinant
Type
Protein
Purity
>90% by SDS-PAGE
Endotoxin concentration
<1 EU/µg
Activity
ED50 = 0.5 - 5 ng/mL; determined by dose-dependent proliferation of human TF-1 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Dry ice
Product Specific Information
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized recombinant human Thrombopoietin in PBS containing 0.1% human serum albumin. Further dilutions should be made in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage: Store this lyophilized preparation at <-20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at <-20°C. Avoid repeated freeze/thaw cycles.
Target Information
Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; ML; MPL ligand; Myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin; Thrombopoietin
Gene Aliases: MGDF; MKCSF; ML; MPLLG; THCYT1; THPO; TPO
UniProt ID: (Human) P40225
Entrez Gene ID: (Human) 7066
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