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Product Details
RP-94514
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
KVPEEQQHLLFRGQLLEDDKHLSDYCIGPNASINVIMQPLEKMALKEAHQPQTQPLWHQLGLVLAKHFEP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (74%), Rat (74%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-56716. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Ubiquitin is a 77 amino acid protein that targets proteins for degradation by the 26S proteasome. Ubiquitin-like proteins are not directly involved in protein degradation, but appear to have many mechanistic similarities with the ubiquitin pathway. UBL4B (Ubiquitin-like protein 4B) is a 174 amino acid cytoplasmic protein that contains a ubiquitin-like domain. UBL4B likely arose by retroposition during mammalian evolution from UBL4A, an X-linked intron-bearing housekeeping gene. While UBL4A is highly conserved, UBL4B has undergone rapid evolution and may have evolved new functions. Expression of UBL4B is restricted to post-meiotic germ cells in testis and ovarian tissue, where it likely functions in post-translational protein modification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ25690; ubiquitin-like 4B; Ubiquitin-like protein 4B
Gene Aliases: UBL4B
UniProt ID: (Human) Q8N7F7
Entrez Gene ID: (Human) 164153
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