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Product Details
RP-88175
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human ULBP1, amino acids Met1-Gly216 (Accession # NP_079494.1) with a C-terminal His-tagged human IgG1 Fc region
Tag
Fc-tag, His-tag
Molecular weight
55-60 kDa
Class
Recombinant
Type
Protein
Purity
>95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized human His-NKG2D (78-216) at 2 µg/ml (100 µL/well) can bind human µLBP1-Fch, The EC50 of human µLBP1-Fch is 8-24 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
ULBP1 is the ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALCAN-beta; N2DL-1; NKG2D ligand; NKG2D ligand 1; NKG2D ligand1; Retinoic acid early transcript 1I; UL16-binding protein 1; ULBP; ULBP 1
Gene Aliases: N2DL-1; N2DL1; NKG2DL1; RAET1I; ULBP1
UniProt ID: (Human) Q9BZM6
Entrez Gene ID: (Human) 80329
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