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Product Details
RP-88182
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human VEGFR3, amino acids Met1-Ile776 (Accession # NP_002011.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
130 kDa
Class
Recombinant
Type
Protein
Purity
>97% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human VEGF-C at 10 µg/mL (100 µL/well) can bind human VEGFR3-his. The EC50 of human VEGFR3-his is 0.011 µg/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
Predicts a molecular mass of 86 kDa. As a result of glycosylation, rhVEGFR3 migrates as an approximately 130 kDa in non-reduced SDS-PAGE.
Reconstituted with sterile deionized water to 0.25 mg/mL
Concentration is measured by UV.
Target Information
Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLT-4; Fms-like tyrosine kinase 4; fms-related tyrosine kinase 4; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; VEGF R3; VEGFR-3
Gene Aliases: FLT-4; FLT4; FLT41; LMPH1A; PCL; VEGFR-3; VEGFR3
UniProt ID: (Human) P35916
Entrez Gene ID: (Human) 2324
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