Gibco

Human Vitronectin, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-140-09-500UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human

Expression System

HEK293 cells

Amino acid sequence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Molecular weight

52.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Recombinant human Vitronectin promotes attachment of hESC and iPSC in serum-free, feeder conditions at 5ug/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human Vitronectin is a 459 amino acid, single-chain, monomeric protein, which migrates at an apparent molecular weight of 75 kDa by SDS-PAGE under reducing conditions. The calculated molecular weight of Recombinant Human Vitronectin is 52.2 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Accelerated differentiation of human induced pluripotent stem cells into regionally specific dorsal and ventral spinal neural progenitor cells for application in spinal cord therapeutics.

Frontiers in neuroscience

Huntemer-Silveira A,Malone D,Frie A,Walsh P,Parr AM

AF-140-09 was used in Cell Culture to describe here the production of a diverse population of both ventral spinal and dorsal horn progenitor cells.

Sat Feb 10 00:00:00 EST 2024

The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury.

Neurobiology of disease

Triplet EM,Kim HN,Yoon H,Radulovic M,Kleppe L,Simon WL,Choi CI,Walsh PJ,Dutton JR,Scarisbrick IA

AF-140-09 was used in In vitro experiments to identify the thrombin receptor (Protease Activated Receptor 1 (PAR1)) as an integral facet of this machine with roles in regulating neurite growth through a growth factor- and cholesterol-dependent mechanism.

Sat May 01 00:00:00 EDT 2021

Automating Human Induced Pluripotent Stem Cell Culture and Differentiation of iPSC-Derived Retinal Pigment Epithelium for Personalized Drug Testing.

SLAS technology

Truong V,Viken K,Geng Z,Barkan S,Johnson B,Ebeling MC,Montezuma SR,Ferrington DA,Dutton JR

AF-140-09 was used in Cell Culture to demonstrate scalable, reproducible culture and differentiation of hiPSC lines from individuals on the TECAN Fluent platform and illustrate the potential for end-to-end automation of hiPSC-based personalized drug testing.

Tue Jun 01 00:00:00 EDT 2021

Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population.

Stem cells (Dayton, Ohio)

Walsh P,Truong V,Nayak S,Saldías Montivero M,Low WC,Parr AM,Dutton JR

Sun Nov 01 00:00:00 EDT 2020

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.

Journal of cellular and molecular medicine

Kawamura M,Yamamoto T,Yamashiro K,Kochi S,Yoshihara-Hirata C,Ideguchi H,Aoyagi H,Omori K,Takashiba S

Fri Feb 01 00:00:00 EST 2019

Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration.

PloS one

Geng Z,Walsh PJ,Truong V,Hill C,Ebeling M,Kapphahn RJ,Montezuma SR,Yuan C,Roehrich H,Ferrington DA,Dutton JR

Thu Aug 17 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: complement S-protein; epibolin; S-protein; serum spreading factor; Serum-spreading factor; somatomedin B; V75; Vitronectin; VN