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Mouse BMP-4 Recombinant Protein, PeproTech®

Mouse BMP-4 Protein in Functional Assay (Functional)

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Mouse BMP-4 Protein (315-27-10UG) in Functional

Bioassay analysis of Mouse BMP-4 Recombinant Protein, PeproTech® (Product # 315-27-1MG).

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Product Details

315-27-10UG

Applications

Tested Dilution

Publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 17 publications 17 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Mouse

Published species

Human, Mouse

Expression System

E. coli

Amino acid sequence

KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR

Molecular weight

23.9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5-15 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

315-27-1MG will be provided as 2 x 500 µg (315-27-500UG).

PeproTech's E.coli derived murine BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits which correspond to amino acids 303-408 of the full length BMP-4 precursor. The calculated molecular weight of Recombinant Murine BMP-4 is 23.9 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of PDGFRA⁺ cells and a CD55⁺ PDGFRA^Lo fraction in the gastric mesenchymal niche.

Nature communications

Manieri E,Tie G,Malagola E,Seruggia D,Madha S,Maglieri A,Huang K,Fujiwara Y,Zhang K,Orkin SH,Wang TC,He R,McCarthy N,Shivdasani RA

315-27 was used in Cell Culture to shed light on spatial, molecular, and functional organization of gastric mesenchyme and the spectrum of signaling sources for epithelial support.

Sat Dec 02 00:00:00 EST 2023

Protocols to generate and isolate mouse myogenic progenitors both in vitro and in vivo.

STAR protocols

Khateb M,Feng X,Dell'Orso S,Sartorelli V

315-27 was used in Cell Culture to describe here a procedure to obtain, dissect, and fluorescence-activated cell sorting (FACS)-isolate somitic cells from genetically labeled Pax7+/Cre; Rosa26YFP/+ embryos.

Fri Mar 17 00:00:00 EDT 2023

Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and development.

Communications biology

Freitag K,Eede P,Ivanov A,Sterczyk N,Schneeberger S,Borodina T,Sauer S,Beule D,Heppner FL

315-27 was used in Cell Culture to highlight the need to carefully consider the choice of astrocyte origin and phenotype with respect to age, isolation and culture protocols based on the respective biological question.

Fri Jan 13 00:00:00 EST 2023

A pendulum of induction between the epiblast and extra-embryonic endoderm supports post-implantation progression.

Development (Cambridge, England)

Vrij EJ,Scholte Op Reimer YS,Roa Fuentes L,Misteli Guerreiro I,Holzmann V,Frias Aldeguer J,Sestini G,Koo BK,Kind J,van Blitterswijk CA,Rivron NC

315-27 was used in In vitro experiments to create a partial mouse embryo model to elucidate the principles of epiblast (Epi) and extra-embryonic endoderm co-development (XEn) to suggest that self-organization can arise from lineage bifurcation followed by a pendulum of induction that propagates over time.

Sat Oct 15 00:00:00 EDT 2022

Effects of bone morphogenetic protein 4 on TGF-β1-induced cell proliferation, apoptosis, activation and differentiation in mouse lung fibroblasts via ERK/p38 MAPK signaling pathway.

PeerJ

Cai Z,Guo H,Qian J,Liu W,Li Y,Yuan L,Zhou Y,Lin R,Xie X,Yang Q,Wu G,Li Q,Zhao L,Liu F,Wang J,Lu W

315-27 was used in Sample Preparation to investigate the effects of bone morphogenetic protein 4 on TGF-β1 cells in mouse primary lung fibroblasts.

Tue Feb 14 00:00:00 EST 2023

Transcriptomics, regulatory syntax, and enhancer identification in mesoderm-induced ESCs at single-cell resolution.

Cell reports

Khateb M,Perovanovic J,Ko KD,Jiang K,Feng X,Acevedo-Luna N,Chal J,Ciuffoli V,Genzor P,Simone J,Haase AD,Pourquié O,Dell'Orso S,Sartorelli V

315-27 was used in In vitro experiments to provide a resource for the discovery of genomic and transcriptional features of pluripotent, mesoderm-induced ESCs and ESC-derived cell lineages.

Tue Aug 16 00:00:00 EDT 2022

Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency.

Nature communications

Shin S,Zhou H,He C,Wei Y,Wang Y,Shingu T,Zeng A,Wang S,Zhou X,Li H,Zhang Q,Mo Q,Long J,Lan F,Chen Y,Hu J

Fri May 21 00:00:00 EDT 2021

Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells.

Communications biology

Rore H,Owen N,Piña-Aguilar RE,Docherty K,Sekido R

Mon Jun 28 00:00:00 EDT 2021

The transcription factor Tfcp2l1 promotes primordial germ cell-like cell specification of pluripotent stem cells.

The Journal of biological chemistry

Zhang M,Ji J,Wang X,Zhang X,Zhang Y,Li Y,Wang X,Li X,Ban Q,Ye SD

Fri Oct 01 00:00:00 EDT 2021

Epigenetic regulation of mammalian Hedgehog signaling to the stroma determines the molecular subtype of bladder cancer.

eLife

Kim S,Kim Y,Kong J,Kim E,Choi JH,Yuk HD,Lee H,Kim HR,Lee KH,Kang M,Roe JS,Moon KC,Kim S,Ku JH,Shin K

315-27 was used in Tissue/organ culture to provide a basis to develop subtype-specific strategies for the management of human bladder cancer.

Tue Apr 30 00:00:00 EDT 2019

Identification of genes under dynamic post-transcriptional regulation from time-series epigenomic data.

Epigenomics

Becker JC,Gérard D,Ginolhac A,Sauter T,Sinkkonen L

315-27 was used in In vitro experiments to predict genes under dynamic post-transcriptional regulation from epigenomic data to identify miR-204 as an early induced adipogenic microRNA targeting Akr1c14 and Il1rl1.

Wed May 01 00:00:00 EDT 2019

Increased FUS levels in astrocytes leads to astrocyte and microglia activation and neuronal death.

Scientific reports

Ajmone-Cat MA,Onori A,Toselli C,Stronati E,Morlando M,Bozzoni I,Monni E,Kokaia Z,Lupo G,Minghetti L,Biagioni S,Cacci E

Thu Mar 14 00:00:00 EDT 2019

Lrp4 expression by adipocytes and osteoblasts differentially impacts sclerostin's endocrine effects on body composition and glucose metabolism.

The Journal of biological chemistry

Kim SP,Da H,Li Z,Kushwaha P,Beil C,Mei L,Xiong WC,Wolfgang MJ,Clemens TL,Riddle RC

Fri Apr 26 00:00:00 EDT 2019

Multiple polycomb epigenetic regulatory proteins are active in normal and regenerating adult olfactory epithelium.

Laryngoscope investigative otolaryngology

Goldstein BJ,Choi R,Goss GM

Mon Oct 01 00:00:00 EDT 2018

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.

Stem cell reports

Kauts ML,Rodriguez-Seoane C,Kaimakis P,Mendes SC,Cortés-Lavaud X,Hill U,Dzierzak E

Tue Jan 09 00:00:00 EST 2018

Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing.

Nature communications

Ishihara J,Ishihara A,Fukunaga K,Sasaki K,White MJV,Briquez PS,Hubbell JA

Mon Jun 04 00:00:00 EDT 2018

Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing.

Nature communications

Ishihara J,Ishihara A,Fukunaga K,Sasaki K,White MJV,Briquez PS,Hubbell JA

Mon Jun 04 00:00:00 EDT 2018

Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy.

Nature biotechnology

Chal J,Oginuma M,Al Tanoury Z,Gobert B,Sumara O,Hick A,Bousson F,Zidouni Y,Mursch C,Moncuquet P,Tassy O,Vincent S,Miyanari A,Bera A,Garnier JM,Guevara G,Hestin M,Kennedy L,Hayashi S,Drayton B,Cherrier T,Gayraud-Morel B,Gussoni E,Relaix F,Tajbakhsh S,Pourquié O

Tue Sep 01 00:00:00 EDT 2015

Rescue of neonatal cardiac dysfunction in mice by administration of cardiac progenitor cells in utero.

Nature communications

Liu X,Hall SRR,Wang Z,Huang H,Ghanta S,Di Sante M,Leri A,Anversa P,Perrella MA

Mon Nov 23 00:00:00 EST 2015

Generation of iPSCs from genetically corrected Brca2 hypomorphic cells: implications in cell reprogramming and stem cell therapy.

Stem cells (Dayton, Ohio)

Navarro S,Moleiro V,Molina-Estevez FJ,Lozano ML,Chinchon R,Almarza E,Quintana-Bustamante O,Mostoslavsky G,Maetzig T,Galla M,Heinz N,Schiedlmeier B,Torres Y,Modlich U,Samper E,Río P,Segovia JC,Raya A,Güenechea G,Izpisua-Belmonte JC,Bueren JA

Sat Feb 01 00:00:00 EST 2014

The transcription factors Tbx18 and Wt1 control the epicardial epithelial-mesenchymal transition through bi-directional regulation of Slug in murine primary epicardial cells.

PloS one

Takeichi M,Nimura K,Mori M,Nakagami H,Kaneda Y

Thu Aug 29 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: BMP; BMP-2B; BMP-4; Bone morphogenetic protein; Bone morphogenetic protein 2B; Bone morphogenetic protein 4

Mouse BMP-4 Recombinant Protein, PeproTech®

Mouse BMP-4 Protein (315-27-10UG) in Functional

Product Details

315-27-10UG

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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