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Product Details
PMC1474
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
Mouse CCL21 recombinant protein contains 110 amino acids
Molecular weight
12 kDa
Class
Recombinant
Type
Protein
Purity
≥98% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 = 10 - 100 ng/mL; determined by the dose-dependent chemotaxis of human activated T-cells in the presence of IL-2.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute this protein in sterile, distilled water to a concentration of 0.1-1.0 mg/mL. Do not vortex. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1.0% BSA.
Storage: This lyophilized preparation is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C for 3months. Avoid repeated freeze/thaw cycles
Target Information
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 6 Ckine; 6Ckine; beta chemokine exodus-2; Beta-chemokine exodus-2; C-C motif chemokine 21a; C-C motif chemokine 21b; C-C motif chemokine 21c; CC chemokine 6Ckine-ser; Ccl21-leu; CCL21-Ser; exodus-2; secondary lymphoid organ chemokine; small inducible cytokine A21; small inducible cytokine A21a; small inducible cytokine A21c; Small-inducible cytokine A21a; Small-inducible cytokine A21b; Small-inducible cytokine A21c; TCA4; Thymus-derived chemotactic agent 4
Gene Aliases: 6CKBAC1; 6CKBAC2; 6Ckine; AI159701; ALP; AW987545; Ccl21a; Ccl21b; Ccl21c; CKb9; exodus-2; plt; Scya21; SCYA21a; Scya21b; Scya21c; SLC; TCA4
UniProt ID: (Mouse) P84444, (Mouse) P86792
Entrez Gene ID: (Mouse) 18829, (Mouse) 100042493, (Mouse) 65956
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