Gibco

Mouse IFN-gamma, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-315-05-100UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 23 publications 23 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Mouse

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Molecular weight

15.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED50 is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine IFN-gamma is a 134 amino-acid length protein with a predicted molecular weight of 15.6 kDa. C-terminal truncation due to proteolytic cleavage occurs during E. coli fermentation and results in a 15.1 kDa protein confirmed by mass spectroscopy. SDS-PAGE analysis of Recombinant Murine IFN-gamma shows a band that migrates at approximately 11-12 kDa, under non-reducing and reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRPA1 deficiency aggravates dilated cardiomyopathy by promoting S100A8 expression to induce M1 macrophage polarization in rats.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Wang M,Zhao M,Zheng Z,Pan W,Zhang J,Yin Z,Wei C,Xu Y,Wan J

AF-315-05 was used in Cell Culture to suggest that TRPA1 deficiency aggravates DCM by promoting S100A8 expression to induce M1 macrophage polarization and cardiac apoptosis.

Thu Jun 01 00:00:00 EDT 2023

Histone deacetylase-2 controls IL-1β production through the regulation of NLRP3 expression and activation in tuberculosis infection.

iScience

Moreira JD,Iakhiaev A,Vankayalapati R,Jung BG,Samten B

AF-315-05 was used in In vitro experiments to suggest that HDAC2, but not HDAC1, controls IL-1β production through NLRP3 inflammasome activation, a mechanism with a significance in chronic inflammatory diseases including tuberculosis.

Fri Aug 19 00:00:00 EDT 2022

DsbA-L deficiency in T cells promotes diet-induced thermogenesis through suppressing IFN-γ production.

Nature communications

Zhou H,Peng X,Hu J,Wang L,Luo H,Zhang J,Zhang Y,Li G,Ji Y,Zhang J,Bai J,Liu M,Zhou Z,Liu F

Tue Jan 12 00:00:00 EST 2021

Personalized cancer vaccine strategy elicits polyfunctional T cells and demonstrates clinical benefits in ovarian cancer.

NPJ vaccines

Tanyi JL,Chiang CL,Chiffelle J,Thierry AC,Baumgartener P,Huber F,Goepfert C,Tarussio D,Tissot S,Torigian DA,Nisenbaum HL,Stevenson BJ,Guiren HF,Ahmed R,Huguenin-Bergenat AL,Zsiros E,Bassani-Sternberg M,Mick R,Powell DJ,Coukos G,Harari A,Kandalaft LE

Mon Mar 15 00:00:00 EDT 2021

The mitochondrial protein ERAL1 suppresses RNA virus infection by facilitating RIG-I-like receptor signaling.

Cell reports

Li S,Kuang M,Chen L,Li Y,Liu S,Du H,Cao L,You F

Tue Jan 19 00:00:00 EST 2021

miR-130b-3p regulates M1 macrophage polarization via targeting IRF1.

Journal of cellular physiology

Guo Q,Zhu X,Wei R,Zhao L,Zhang Z,Yin X,Zhang Y,Chu C,Wang B,Li X

AF-315-05 was used in In vitro experiments to show that miR-130b-3p affected the polarization of M1 macrophages by directly inhibiting Irf1such that the miR-130b-3p/IRF1 pathway may be a potential target for regulating macrophage polarization.

Mon Mar 01 00:00:00 EST 2021

PTPN2 phosphatase deletion in T cells promotes anti-tumour immunity and CAR T-cell efficacy in solid tumours.

The EMBO journal

Wiede F,Lu KH,Du X,Liang S,Hochheiser K,Dodd GT,Goh PK,Kearney C,Meyran D,Beavis PA,Henderson MA,Park SL,Waithman J,Zhang S,Zhang ZY,Oliaro J,Gebhardt T,Darcy PK,Tiganis T

Wed Jan 15 00:00:00 EST 2020

Targeting EZH2 Enhances Antigen Presentation, Antitumor Immunity, and Circumvents Anti-PD-1 Resistance in Head and Neck Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Zhou L,Mudianto T,Ma X,Riley R,Uppaluri R

AF-315-05 was used in Sample Preparation (Western Blotting) to investigate EZH2 as a target for treatment of HNSCC in human and mouse cells.

Wed Jan 01 00:00:00 EST 2020

Sclerostin expression in trabecular bone is downregulated by osteoclasts.

Scientific reports

Koide M,Yamashita T,Murakami K,Uehara S,Nakamura K,Nakamura M,Matsushita M,Ara T,Yasuda H,Penninger JM,Takahashi N,Udagawa N,Kobayashi Y

Thu Aug 13 00:00:00 EDT 2020

NR1D1 modulates synovial inflammation and bone destruction in rheumatoid arthritis.

Cell death & disease

Liu H,Zhu Y,Gao Y,Qi D,Zhao L,Zhao L,Liu C,Tao T,Zhou C,Sun X,Guo F,Xiao J

Tue Feb 18 00:00:00 EST 2020

Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells.

Scientific reports

Huang K,Masuda A,Chen G,Bushra S,Kamon M,Araki T,Kinoshita M,Ohkawara B,Ito M,Ohno K

Thu Feb 13 00:00:00 EST 2020

Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues.

Nature communications

Gajdasik DW,Gaspal F,Halford EE,Fiancette R,Dutton EE,Willis C,Rückert T,Romagnani C,Gerard A,Bevington SL,MacDonald AS,Botto M,Vyse T,Withers DR

Thu Jul 09 00:00:00 EDT 2020

Silencing lncRNA Lfar1 alleviates the classical activation and pyoptosis of macrophage in hepatic fibrosis.

Cell death & disease

Zhang K,Shi Z,Zhang M,Dong X,Zheng L,Li G,Han X,Yao Z,Han T,Hong W

Tue Feb 18 00:00:00 EST 2020

High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange.

Nature communications

Overall SA,Toor JS,Hao S,Yarmarkovich M,Morozov GI,Nguyen S,Japp AS,Gonzalez N,Moschidi D,Betts MR,Maris JM,Smibert P,Sgourakis NG

Mon Apr 20 00:00:00 EDT 2020

Transcription factor 21 expression in injured podocytes of glomerular diseases.

Scientific reports

Usui J,Yaguchi M,Yamazaki S,Takahashi-Kobayashi M,Kawamura T,Kaneko S,Seshan SV,Ronco P,Yamagata K

Mon Jul 13 00:00:00 EDT 2020

Programmed cell death ligand 1 disruption by clustered regularly interspaced short palindromic repeats/Cas9-genome editing promotes antitumor immunity and suppresses ovarian cancer progression.

Cancer science

Yahata T,Mizoguchi M,Kimura A,Orimo T,Toujima S,Kuninaka Y,Nosaka M,Ishida Y,Sasaki I,Fukuda-Ohta Y,Hemmi H,Iwahashi N,Noguchi T,Kaisho T,Kondo T,Ino K

Mon Apr 01 00:00:00 EDT 2019

Growth hormone-mediated reprogramming of macrophage transcriptome and effector functions.

Scientific reports

Schneider A,Wood HN,Geden S,Greene CJ,Yates RM,Masternak MM,Rohde KH

Wed Dec 18 00:00:00 EST 2019

Dietary tryptophan links encephalogenicity of autoreactive T cells with gut microbial ecology.

Nature communications

Sonner JK,Keil M,Falk-Paulsen M,Mishra N,Rehman A,Kramer M,Deumelandt K,Röwe J,Sanghvi K,Wolf L,von Landenberg A,Wolff H,Bharti R,Oezen I,Lanz TV,Wanke F,Tang Y,Brandao I,Mohapatra SR,Epping L,Grill A,Röth R,Niesler B,Meuth SG,Opitz CA,Okun JG,Reinhardt C,Kurschus FC,Wick W,Bode HB,Rosenstiel P,Platten M

Fri Oct 25 00:00:00 EDT 2019

Oligodendrocyte precursor cells present antigen and are cytotoxic targets in inflammatory demyelination.

Nature communications

Kirby L,Jin J,Cardona JG,Smith MD,Martin KA,Wang J,Strasburger H,Herbst L,Alexis M,Karnell J,Davidson T,Dutta R,Goverman J,Bergles D,Calabresi PA

Thu Aug 29 00:00:00 EDT 2019

Adipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR -PPARγ Signalling in Macrophages.

Scientific reports

Souza-Moreira L,Soares VC,Dias SDSG,Bozza PT

Mon Dec 30 00:00:00 EST 2019

Modulation of the extrinsic cell death signaling pathway by viral Flip induces acute-death mediated liver failure.

Cell death & disease

Bittel M,Kremer AE,Stürzl M,Wirtz S,Stolzer I,Neurath MF,Ballon G,Günther C

Thu Nov 21 00:00:00 EST 2019

Quorum sensing modulates the formation of virulent Legionella persisters within infected cells.

Nature communications

Personnic N,Striednig B,Lezan E,Manske C,Welin A,Schmidt A,Hilbi H

Mon Nov 18 00:00:00 EST 2019

RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.

Nature communications

Stone AEL,Green R,Wilkins C,Hemann EA,Gale M

Tue Aug 13 00:00:00 EDT 2019

RIPK1 protects hepatocytes from death in Fas-induced hepatitis.

Scientific reports

Filliol A,Farooq M,Piquet-Pellorce C,Genet V,Dimanche-Boitrel MT,Vandenabeele P,Bertrand MJM,Samson M,Le Seyec J

Wed Aug 23 00:00:00 EDT 2017

TLR9 signalling in microglia attenuates seizure-induced aberrant neurogenesis in the adult hippocampus.

Nature communications

Matsuda T,Murao N,Katano Y,Juliandi B,Kohyama J,Akira S,Kawai T,Nakashima K

Mon Mar 09 00:00:00 EDT 2015

NOD2 regulates CXCR3-dependent CD8+ T cell accumulation in intestinal tissues with acute injury.

Journal of immunology (Baltimore, Md. : 1950)

Wu X,Lahiri A,Haines GK,Flavell RA,Abraham C

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g

Mouse IFN-gamma, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-315-05-100UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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