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Product Details
PMC0155
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
Mouse IL-15 recombinant protein contains 115 amino acids
Molecular weight
13.4 kDa
Class
Recombinant
Type
Protein
Purity
≥98% by SDS-PAGE and HPLC
Endotoxin concentration
<0.1 ng/µg
Activity
ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
10mM sodium phosphate, pH 8
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute mouse IL-15 in sterile, distilled water to a concentration of 0.5-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 6 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: il 15; IL-15; ILN; Interleukin; Interleukin-15; Interleukin15; M-IL-15; MGC9721
Gene Aliases: AI503618; IL-15; Il15
UniProt ID: (Mouse) P48346
Entrez Gene ID: (Mouse) 16168
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