Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
PMC0046
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
E. coli
Amino acid sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Molecular weight
13.5 kDa
Class
Recombinant
Type
Protein
Purity
>96% by SDS-PAGE and RP-HPLC
Activity
ED50 ≤ 2 ng/mL; determined by the dose-dependent proliferation of mouse HT-2 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Wet or Dry ice (international)
Product Specific Information
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized mIL-4 should be reconstituted in sterile, 10 mM HAc not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. For long term storage, it is recommended to add a carrier protein, such as 0.1% HSA or BSA.
Storage: This lyophilized preparation is stable at room temperature for 3 weeks, but should be stored desiccated below -18°C. Upon reconstitution, the preparation is stable at 4°C for up to 1 week, but should be stored below -18°C for long term storage. Avoid repeated freeze/thaw cycles.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: BSF-1; Il-4; Il4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support