Gibco

Mouse IL-6, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-216-16-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 11 publications 11 publications

Product Specifications

Species

Mouse

Published species

Mouse

Expression System

E. coli

Amino acid sequence

MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT

Molecular weight

21.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of mouse B9 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine IL-6 is a 21.7 kDa protein containing 188 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reciprocal transcription factor networks govern tissue-resident ILC3 subset function and identity.

Nature immunology

Fiancette R,Finlay CM,Willis C,Bevington SL,Soley J,Ng STH,Baker SM,Andrews S,Hepworth MR,Withers DR

Fri Oct 01 00:00:00 EDT 2021

Default polyfunctional T helper 1 response to ample signal 1 alone.

Cellular & molecular immunology

Danelli L,Cornish G,Merkenschlager J,Kassiotis G

Thu Jul 01 00:00:00 EDT 2021

Lactobacillus rhamnosus attenuates bone loss and maintains bone health by skewing Treg-Th17 cell balance in Ovx mice.

Scientific reports

Sapra L,Dar HY,Bhardwaj A,Pandey A,Kumari S,Azam Z,Upmanyu V,Anwar A,Shukla P,Mishra PK,Saini C,Verma B,Srivastava RK

Tue Jan 19 00:00:00 EST 2021

T-cell expression of Bruton's tyrosine kinase promotes autoreactive T-cell activation and exacerbates aplastic anemia.

Cellular & molecular immunology

Xia S,Liu X,Cao X,Xu S

Thu Oct 01 00:00:00 EDT 2020

Proimmunogenic impact of MEK inhibition synergizes with agonist anti-CD40 immunostimulatory antibodies in tumor therapy.

Nature communications

Baumann D,Hägele T,Mochayedi J,Drebant J,Vent C,Blobner S,Noll JH,Nickel I,Schumacher C,Boos SL,Daniel AS,Wendler S,Volkmar M,Strobel O,Offringa R

Fri May 01 00:00:00 EDT 2020

CD4⁺ T-Cell Differentiation In Vitro.

Methods in molecular biology (Clifton, N.J.)

Yang W,Chen X,Hu H

AF-216-16 was used in In vitro experiments to study the regulating mechanism and function of Th cells, dependent on in vitro CD4+ T-cell differentiation.

Tue Jan 26 00:00:00 EST 2021

TAGAP instructs Th17 differentiation by bridging Dectin activation to EPHB2 signaling in innate antifungal response.

Nature communications

Chen J,He R,Sun W,Gao R,Peng Q,Zhu L,Du Y,Ma X,Guo X,Zhang H,Tan C,Wang J,Zhang W,Weng X,Man J,Bauer H,Wang QK,Martin BN,Zhang CJ,Li X,Wang C

Mon Apr 20 00:00:00 EDT 2020

IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b.

Cell death & disease

Yang R,Huang H,Cui S,Zhou Y,Zhang T,Zhou Y

Thu Jul 30 00:00:00 EDT 2020

Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia.

Nature communications

Minuesa G,Albanese SK,Xie W,Kazansky Y,Worroll D,Chow A,Schurer A,Park SM,Rotsides CZ,Taggart J,Rizzi A,Naden LN,Chou T,Gourkanti S,Cappel D,Passarelli MC,Fairchild L,Adura C,Glickman JF,Schulman J,Famulare C,Patel M,Eibl JK,Ross GM,Bhattacharya S,Tan DS,Leslie CS,Beuming T,Patel DJ,Goldgur Y,Chodera JD,Kharas MG

Wed Jun 19 00:00:00 EDT 2019

Elevated Wall Tension Initiates Interleukin-6 Expression and Abdominal Aortic Dilation.

Annals of vascular surgery

Akerman AW,Stroud RE,Barrs RW,Grespin RT,McDonald LT,LaRue RAC,Mukherjee R,Ikonomidis JS,Jones JA,Ruddy JM

AF-216-16 was used in In vivo experiments to evaluate whether elevated tension may initiate IL-6 production to accumulate monocyte/macrophages and promote dilation of the abdominal aorta (AA).

Mon Jan 01 00:00:00 EST 2018

Role of Erk1/2 signaling in the regulation of neutrophil versus monocyte development in response to G-CSF and M-CSF.

The Journal of biological chemistry

Hu N,Qiu Y,Dong F

Fri Oct 02 00:00:00 EDT 2015

Increased intensity lymphodepletion enhances tumor treatment efficacy of adoptively transferred tumor-specific T cells.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Wrzesinski C,Paulos CM,Kaiser A,Muranski P,Palmer DC,Gattinoni L,Yu Z,Rosenberg SA,Restifo NP

Fri Jan 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

Mouse IL-6, Animal-Free Recombinant Protein, PeproTech®

Product Details

AF-216-16-10UG

Functional Assay (Functional)

In vitro Assay (IV)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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