Gibco

Mouse LIF Recombinant Protein, PeproTech®

Product Details

250-02-25UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Mouse

Published species

Mouse, Rat

Expression System

E. coli

Amino acid sequence

SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

Molecular weight

19.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intranasal mesenchymal stem cell therapy to boost myelination after encephalopathy of prematurity.

Glia

Vaes JEG,van Kammen CM,Trayford C,van der Toorn A,Ruhwedel T,Benders MJNL,Dijkhuizen RM,Möbius W,van Rijt SH,Nijboer CH

250-02 was used in Cell Culture to show that MSCs adapt their secretome after ex vivo exposure to dWMI milieu and identified several factors including IGF1, EGF, LIF, and IL11 that potently boost OL maturation.

Mon Mar 01 00:00:00 EST 2021

LIF maintains mouse embryonic stem cells pluripotency by modulating TET1 and JMJD2 activity in a JAK2-dependent manner.

Stem cells (Dayton, Ohio)

Wulansari N,Sulistio YA,Darsono WHW,Kim CH,Lee SH

Tue Jun 01 00:00:00 EDT 2021

Leukemia Inhibitory Factor Is Required for Subventricular Zone Astrocyte Progenitor Proliferation and for Prokineticin-2 Production after a Closed Head Injury in Mice.

Neurotrauma reports

Frondelli MJ,Levison SW

250-02 was used in Sample Preparation (FC/FACS) to investigate astrocytes in closed head injury.

Tue Jul 06 00:00:00 EDT 2021

Cell cycle heterogeneity directs spontaneous 2C state entry and exit in mouse embryonic stem cells.

Stem cell reports

Zhu Y,Cheng C,Chen L,Zhang L,Pan H,Hou L,Sun Z,Zhang L,Fu X,Chan KY,Zhang J

Tue Nov 09 00:00:00 EST 2021

IL-4 controls activated neutrophil FcγR2b expression and migration into inflamed joints.

Proceedings of the National Academy of Sciences of the United States of America

Panda SK,Wigerblad G,Jiang L,Jiménez-Andrade Y,Iyer VS,Shen Y,Boddul SV,Guerreiro-Cacais AO,Raposo B,Kasza Z,Wermeling F

Tue Feb 11 00:00:00 EST 2020

The detrimental effects of stress-induced glucocorticoid exposure on mouse uterine receptivity and decidualization.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zheng HT,Zhang HY,Chen ST,Li MY,Fu T,Yang ZM

250-02 was used in In vivo experiments to explore the effects of GCs exposure on uterine receptivity, decidualization, and their underlying mechanisms in mice.

Sun Nov 01 00:00:00 EDT 2020

Hemi-methylated CpG sites connect Dnmt1-knockdown-induced and Tet1-induced DNA demethylation during somatic cell reprogramming.

Cell discovery

He S,Wang F,Zhang Y,Chen J,Liang L,Li Y,Zhang M,Yang X,Pang H,Li Y,Huang X,Qin D,Pei D,Sun H,Zheng H

Tue Sep 29 00:00:00 EDT 2020

Cardiotrophin-like cytokine (CLCF1) modulates mesenchymal stem cell osteoblastic differentiation.

The Journal of biological chemistry

Nahlé S,Pasquin S,Laplante V,Rousseau F,Sharma M,Gauchat JF

Fri Aug 09 00:00:00 EDT 2019

MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis.

Experimental and therapeutic medicine

Ouyang L,Dan Y,Shao Z,Yang S,Yang C,Liu G,Duan D

250-02 was used in In vitro experiments to provide a potential novel treatment approach for soft tissue defects such as bone exposure, chronic skin ulcers, bedsores, limb necrosis, osteonecrosis and other ischemic diseases.

Tue Oct 01 00:00:00 EDT 2019

A tunable dual-input system for on-demand dynamic gene expression regulation.

Nature communications

Pedone E,Postiglione L,Aulicino F,Rocca DL,Montes-Olivas S,Khazim M,di Bernardo D,Pia Cosma M,Marucci L

Wed Oct 02 00:00:00 EDT 2019

GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1.

Cell death and differentiation

Guo S,Zhang Y,Zhou T,Wang D,Weng Y,Chen Q,Ma J,Li YP,Wang L

Thu Nov 01 00:00:00 EDT 2018

Critical role of OX40 in the expansion and survival of CD4 T-cell-derived double-negative T cells.

Cell death & disease

Sun G,Sun X,Li W,Liu K,Tian D,Dong Y,Sun X,Xu H,Zhang D

Wed May 23 00:00:00 EDT 2018

Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer.

The Journal of experimental medicine

Öhlund D,Handly-Santana A,Biffi G,Elyada E,Almeida AS,Ponz-Sarvise M,Corbo V,Oni TE,Hearn SA,Lee EJ,Chio II,Hwang CI,Tiriac H,Baker LA,Engle DD,Feig C,Kultti A,Egeblad M,Fearon DT,Crawford JM,Clevers H,Park Y,Tuveson DA

Mon Mar 06 00:00:00 EST 2017

Blocking gp130 signaling suppresses autotaxin expression in adipocytes and improves insulin sensitivity in diet-induced obesity.

Journal of lipid research

Sun S,Wang R,Song J,Guan M,Li N,Zhang X,Zhao Z,Zhang J

Wed Nov 01 00:00:00 EDT 2017

The distribution and adipogenic potential of perivascular adipose tissue adipocyte progenitors is dependent on sexual dimorphism and vessel location.

Physiological reports

Contreras GA,Thelen K,Ayala-Lopez N,Watts SW

250-02 was used in Cell Culture to test the hypothesis that vessel location and sexual dimorphism affect the distribution and adipogenic capacity of APC in cardiovascular disease risk relevant PVAT sites.

Sat Oct 01 00:00:00 EDT 2016

The anti-apoptotic Bcl-2 family protein A1/Bfl-1 regulates neutrophil survival and homeostasis and is controlled via PI3K and JAK/STAT signaling.

Cell death & disease

Vier J,Groth M,Sochalska M,Kirschnek S

Thu Feb 18 00:00:00 EST 2016

Autophagy confers DNA damage repair pathways to protect the hematopoietic system from nuclear radiation injury.

Scientific reports

Lin W,Yuan N,Wang Z,Cao Y,Fang Y,Li X,Xu F,Song L,Wang J,Zhang H,Yan L,Xu L,Zhang X,Zhang S,Wang J

Tue Jul 21 00:00:00 EDT 2015

Signaling axis involving Hedgehog, Notch, and Scl promotes the embryonic endothelial-to-hematopoietic transition.

Proceedings of the National Academy of Sciences of the United States of America

Kim PG,Albacker CE,Lu YF,Jang IH,Lim Y,Heffner GC,Arora N,Bowman TV,Lin MI,Lensch MW,De Los Angeles A,Zon LI,Loewer S,Daley GQ

Tue Jan 08 00:00:00 EST 2013

Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells.