Gibco

Mouse MCP-5 (CCL12) Recombinant Protein, PeproTech®

Product Details

250-04-20UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Mouse

Published species

Bacteria, Human, Mouse

Expression System

E. coli

Amino acid sequence

GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG

Molecular weight

9.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine MCP-5 is a 9.3 kDa protein containing 82 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Atypical Chemokine Receptor Ackr2 Constrains NK Cell Migratory Activity and Promotes Metastasis.

Journal of immunology (Baltimore, Md. : 1950)

Hansell CAH,Fraser AR,Hayes AJ,Pingen M,Burt CL,Lee KM,Medina-Ruiz L,Brownlie D,Macleod MKL,Burgoyne P,Wilson GJ,Nibbs RJB,Graham GJ

Mon Oct 15 00:00:00 EDT 2018

Yeast surface display identifies a family of evasins from ticks with novel polyvalent CC chemokine-binding activities.

Scientific reports

Singh K,Davies G,Alenazi Y,Eaton JRO,Kawamura A,Bhattacharya S

Tue Jun 27 00:00:00 EDT 2017

Systematic Screening of Chemokines to Identify Candidates to Model and Create Ectopic Lymph Node Structures for Cancer Immunotherapy.

Scientific reports

Yagawa Y,Robertson-Tessi M,Zhou SL,Anderson ARA,Mulé JJ,Mailloux AW

Wed Nov 22 00:00:00 EST 2017

Characterization of conventional and atypical receptors for the chemokine CCL2 on mouse leukocytes.

Journal of immunology (Baltimore, Md. : 1950)

Ford LB,Cerovic V,Milling SW,Graham GJ,Hansell CA,Nibbs RJ

Tue Jul 01 00:00:00 EDT 2014

Chemokine (C-C motif) ligand 2 mediates direct and indirect fibrotic responses in human and murine cultured fibrocytes.

Fibrogenesis & tissue repair

Ekert JE,Murray LA,Das AM,Sheng H,Giles-Komar J,Rycyzyn MA

Wed Oct 19 00:00:00 EDT 2011

Host chemokines bind to Staphylococcus aureus and stimulate protein A release.

The Journal of biological chemistry

Yung SC,Parenti D,Murphy PM

Fri Feb 18 00:00:00 EST 2011

DNA damage-mediated induction of a chemoresistant niche.

Cell

Gilbert LA,Hemann MT

Fri Oct 29 00:00:00 EDT 2010

A novel recombinant fusion protein encoding a 20-amino acid residue of the third extracellular (E3) domain of CCR2 neutralizes the biological activity of CCL2.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Zohar Y,Anunu R,Klapper L,Elkeles A,Seagal J,Yefenof E,Ayalon-Soffer M,Karin N

Wed Jul 01 00:00:00 EDT 2009

Structure and function of A41, a vaccinia virus chemokine binding protein.

PLoS pathogens

Bahar MW,Kenyon JC,Putz MM,Abrescia NG,Pease JE,Wise EL,Stuart DI,Smith GL,Grimes JM

Tue Jan 01 00:00:00 EST 2008

Regulation of trafficking receptor expression in human forkhead box P3+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Broxmeyer HE,Kim CH

Sat Jul 15 00:00:00 EDT 2006

Differential requirements for DOCK2 and phosphoinositide-3-kinase gamma during T and B lymphocyte homing.

Immunity

Nombela-Arrieta C,Lacalle RA,Montoya MC,Kunisaki Y,Megías D,Marqués M,Carrera AC,Mañes S,Fukui Y,Martínez-A C,Stein JV

Wed Sep 01 00:00:00 EDT 2004

Interleukin 4 and 13 participation in mycobacterial (type-1) and schistosomal (type-2) antigen-elicited pulmonary granuloma formation: multiparameter analysis of cellular recruitment, chemokine expression and cytokine networks.

Cytokine

Ruth JH,Warmington KS,Shang X,Lincoln P,Evanoff H,Kunkel SL,Chensue SW

Mon May 01 00:00:00 EDT 2000

Disturbed CD4+ T cell homeostasis and in vitro HIV-1 susceptibility in transgenic mice expressing T cell line-tropic HIV-1 receptors.

The Journal of experimental medicine

Sawada S,Gowrishankar K,Kitamura R,Suzuki M,Suzuki G,Tahara S,Koito A

Mon May 04 00:00:00 EDT 1998

Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1.

The Journal of experimental medicine

Sarafi MN,Garcia-Zepeda EA,MacLean JA,Charo IF,Luster AD

Mon Jan 06 00:00:00 EST 1997

Bioinformatics

Protein Aliases: C-C motif chemokine 12; MCP-1-related chemokine; MCP-5; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; small inducible cytokine A12; Small-inducible cytokine A12

Mouse MCP-5 (CCL12) Recombinant Protein, PeproTech®

Product Details

250-04-20UG

Western Blot (WB)

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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