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Product Details
A42615
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse PD-L1, amino acids Met1-Thr238 (Accession # NP_068693.1) with a C-terminal His-tag
Tag
His-tag
Molecular weight
26.3 kDa
Class
Recombinant
Type
Protein
Purity
>98 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-L1 at 1 µg/mL (100 µL/well) can bind mouse PD1 with a linear range of 6.25-400 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% Tween 80, 5% mannitol, 5% trehalose
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1
UniProt ID: (Mouse) Q9EP73
Entrez Gene ID: (Mouse) 60533
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