Gibco

Mouse PDGF-AA Recombinant Protein, PeproTech®

Product Details

315-17-10UG

Applications

Tested Dilution

Publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 29 publications 29 publications

Miscellaneous PubMed (Misc)

View 6 publications 6 publications

Product Specifications

Species

Mouse

Published species

Mouse, Rat

Expression System

E. coli

Amino acid sequence

SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATSNLNP DHREEETGRR RESGKNRKRK RLKPT

Molecular weight

28.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interleukin 13 promotes long-term recovery after ischemic stroke by inhibiting the activation of STAT3.

Journal of neuroinflammation

Chen D,Li J,Huang Y,Wei P,Miao W,Yang Y,Gao Y

315-17 was used in Cell Culture to conclude that IL-13 promotes white matter repair and improves neurofunctional outcomes after ischemic stroke by modulating microglia/macrophages via inhibition of STAT3 phosphorylation.

Mon May 16 00:00:00 EDT 2022

Interleukin 13 promotes long-term recovery after ischemic stroke by inhibiting the activation of STAT3.

Journal of neuroinflammation

Chen D,Li J,Huang Y,Wei P,Miao W,Yang Y,Gao Y

Mon May 16 00:00:00 EDT 2022

Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.

Scientific reports

Boshans LL,Soh H,Wood WM,Nolan TM,Mandoiu II,Yanagawa Y,Tzingounis AV,Nishiyama A

Thu Feb 11 00:00:00 EST 2021

Protease-Triggered Release of Stabilized CXCL12 from Coated Scaffolds in an Ex Vivo Wound Model.

Pharmaceutics

Spiller S,Wippold T,Bellmann-Sickert K,Franz S,Saalbach A,Anderegg U,Beck-Sickinger AG

315-17 was used in Sample Preparation (FC/FACS) to display successful application of protein-coated biomaterials, using protease-mediated-release of stabalized CXCL12, for skin regeneration grafts.

Fri Oct 01 00:00:00 EDT 2021

PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.

Cell reports

Wang Y,Zhang Y,Zhang S,Kim B,Hull VL,Xu J,Prabhu P,Gregory M,Martinez-Cerdeno V,Zhan X,Deng W,Guo F

Tue Oct 05 00:00:00 EDT 2021

Bile acid metabolism is altered in multiple sclerosis and supplementation ameliorates neuroinflammation.

The Journal of clinical investigation

Bhargava P,Smith MD,Mische L,Harrington E,Fitzgerald KC,Martin K,Kim S,Reyes AA,Gonzalez-Cardona J,Volsko C,Tripathi A,Singh S,Varanasi K,Lord HN,Meyers K,Taylor M,Gharagozloo M,Sotirchos ES,Nourbakhsh B,Dutta R,Mowry EM,Waubant E,Calabresi PA

Wed Jul 01 00:00:00 EDT 2020

Glial type specific regulation of CNS angiogenesis by HIFα-activated different signaling pathways.

Nature communications

Zhang S,Kim B,Zhu X,Gui X,Wang Y,Lan Z,Prabhu P,Fond K,Wang A,Guo F

Fri Apr 24 00:00:00 EDT 2020

Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers.

FEBS open bio

Kazi T,Niibe I,Nishikawa A,Matsuzaki T

Sat Feb 01 00:00:00 EST 2020

microRNA-875-5p plays critical role for mesenchymal condensation in epithelial-mesenchymal interaction during tooth development.

Scientific reports

Funada K,Yoshizaki K,MIyazaki K,Han X,Yuta T,Tian T,Mizuta K,Fu Y,Iwamoto T,Yamada A,Takahashi I,Fukumoto S

Wed Mar 18 00:00:00 EDT 2020

Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury.

Nature communications

Floriddia EM,Lourenço T,Zhang S,van Bruggen D,Hilscher MM,Kukanja P,Gonçalves Dos Santos JP,Altınkök M,Yokota C,Llorens-Bobadilla E,Mulinyawe SB,Grãos M,Sun LO,Frisén J,Nilsson M,Castelo-Branco G

Tue Nov 17 00:00:00 EST 2020

Muscarinic receptors modulate Nerve Growth Factor production in rat Schwann-like adipose-derived stem cells and in Schwann cells.

Scientific reports

Piovesana R,Faroni A,Taggi M,Matera A,Soligo M,Canipari R,Manni L,Reid AJ,Tata AM

Tue Apr 28 00:00:00 EDT 2020

Methotrexate Chemotherapy Induces Persistent Tri-glial Dysregulation that Underlies Chemotherapy-Related Cognitive Impairment.

Cell

Gibson EM,Nagaraja S,Ocampo A,Tam LT,Wood LS,Pallegar PN,Greene JJ,Geraghty AC,Goldstein AK,Ni L,Woo PJ,Barres BA,Liddelow S,Vogel H,Monje M

Thu Jan 10 00:00:00 EST 2019

Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms.

The Journal of clinical investigation

Stivala S,Codilupi T,Brkic S,Baerenwaldt A,Ghosh N,Hao-Shen H,Dirnhofer S,Dettmer MS,Simillion C,Kaufmann BA,Chiu S,Keller M,Kleppe M,Hilpert M,Buser AS,Passweg JR,Radimerski T,Skoda RC,Levine RL,Meyer SC

Mon Mar 04 00:00:00 EST 2019

Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms.

The Journal of clinical investigation

Mon Mar 04 00:00:00 EST 2019

Inhibition of MAPK/ERK pathway promotes oligodendrocytes generation and recovery of demyelinating diseases.

Glia

Suo N,Guo YE,He B,Gu H,Xie X

Mon Jul 01 00:00:00 EDT 2019

Oncogenic Ras mutant causes the hyperactivation of NF-κB via acceleration of its transcriptional activation.

Molecular oncology

Tago K,Funakoshi-Tago M,Ohta S,Kawata H,Saitoh H,Horie H,Aoki-Ohmura C,Yamauchi J,Tanaka A,Matsugi J,Yanagisawa K

Fri Nov 01 00:00:00 EDT 2019

Leonurine suppresses neuroinflammation through promoting oligodendrocyte maturation.

Journal of cellular and molecular medicine

Jin M,Li Q,Gu Y,Wan B,Huang J,Xu X,Huang R,Zhang Y

Fri Feb 01 00:00:00 EST 2019

Laminin α2, α4, and α5 Chains Positively Regulate Migration and Survival of Oligodendrocyte Precursor Cells.

Scientific reports

Suzuki N,Hyodo M,Hayashi C,Mabuchi Y,Sekimoto K,Onchi C,Sekiguchi K,Akazawa C

Fri Dec 27 00:00:00 EST 2019

Circuit Design Features of a Stable Two-Cell System.

Cell

Zhou X,Franklin RA,Adler M,Jacox JB,Bailis W,Shyer JA,Flavell RA,Mayo A,Alon U,Medzhitov R

Thu Feb 08 00:00:00 EST 2018

Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing.

Nature communications

Ishihara J,Ishihara A,Fukunaga K,Sasaki K,White MJV,Briquez PS,Hubbell JA

Mon Jun 04 00:00:00 EDT 2018

Laminin heparin-binding peptides bind to several growth factors and enhance diabetic wound healing.

Nature communications

Ishihara J,Ishihara A,Fukunaga K,Sasaki K,White MJV,Briquez PS,Hubbell JA

Mon Jun 04 00:00:00 EDT 2018

Peripherally derived FGF21 promotes remyelination in the central nervous system.

The Journal of clinical investigation

Kuroda M,Muramatsu R,Maedera N,Koyama Y,Hamaguchi M,Fujimura H,Yoshida M,Konishi M,Itoh N,Mochizuki H,Yamashita T

Fri Sep 01 00:00:00 EDT 2017

The DYT6 Dystonia Protein THAP1 Regulates Myelination within the Oligodendrocyte Lineage.

Developmental cell

Yellajoshyula D,Liang CC,Pappas SS,Penati S,Yang A,Mecano R,Kumaran R,Jou S,Cookson MR,Dauer WT

315-17 was used in Cell Culture to study the neurodevelopmental processes in which the transcription factor, THAP1, participates in.

Mon Jul 10 00:00:00 EDT 2017

YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation.

Glia

Shimizu T,Osanai Y,Tanaka KF,Abe M,Natsume R,Sakimura K,Ikenaka K

315-17 was used in Cell Culture to study the molecular mechanisms underlying mechanical regulation of oligodendrocytes (OLs) by YAP.

Wed Feb 01 00:00:00 EST 2017

Osteogenic Factor Runx2 Marks a Subset of Leptin Receptor-Positive Cells that Sit Atop the Bone Marrow Stromal Cell Hierarchy.

Scientific reports

Yang M,Arai A,Udagawa N,Hiraga T,Lijuan Z,Ito S,Komori T,Moriishi T,Matsuo K,Shimoda K,Zahalka AH,Kobayashi Y,Takahashi N,Mizoguchi T

Mon Jul 10 00:00:00 EDT 2017

Fibrinolytic crosstalk with endothelial cells expands murine mesenchymal stromal cells.

Blood

Dhahri D,Sato-Kusubata K,Ohki-Koizumi M,Nishida C,Tashiro Y,Munakata S,Shimazu H,Salama Y,Eiamboonsert S,Nakauchi H,Hattori K,Heissig B

Thu Aug 25 00:00:00 EDT 2016

PI3-kinase mutation linked to insulin and growth factor resistance in vivo.

The Journal of clinical investigation

Winnay JN,Solheim MH,Dirice E,Sakaguchi M,Noh HL,Kang HJ,Takahashi H,Chudasama KK,Kim JK,Molven A,Kahn CR,Njølstad PR

Fri Apr 01 00:00:00 EDT 2016

Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development.

Proceedings of the National Academy of Sciences of the United States of America

Noda K,Kitami M,Kitami K,Kaku M,Komatsu Y

Tue May 10 00:00:00 EDT 2016

Loss of Asxl1 Alters Self-Renewal and Cell Fate of Bone Marrow Stromal Cell, Leading to Bohring-Opitz-like Syndrome in Mice.

Stem cell reports

Zhang P,Xing C,Rhodes SD,He Y,Deng K,Li Z,He F,Zhu C,Nguyen L,Zhou Y,Chen S,Mohammad KS,Guise TA,Abdel-Wahab O,Xu M,Wang QF,Yang FC

Tue Jun 14 00:00:00 EDT 2016

Prostacyclin prevents pericyte loss and demyelination induced by lysophosphatidylcholine in the central nervous system.

The Journal of biological chemistry

Muramatsu R,Kuroda M,Matoba K,Lin H,Takahashi C,Koyama Y,Yamashita T

Fri May 01 00:00:00 EDT 2015

Prostacyclin prevents pericyte loss and demyelination induced by lysophosphatidylcholine in the central nervous system.

The Journal of biological chemistry

Muramatsu R,Kuroda M,Matoba K,Lin H,Takahashi C,Koyama Y,Yamashita T

Fri May 01 00:00:00 EDT 2015

Generation of neural progenitor cells by chemical cocktails and hypoxia.

Cell research

Cheng L,Hu W,Qiu B,Zhao J,Yu Y,Guan W,Wang M,Yang W,Pei G

Sun Jun 01 00:00:00 EDT 2014

A regenerative approach to the treatment of multiple sclerosis.

Nature

Deshmukh VA,Tardif V,Lyssiotis CA,Green CC,Kerman B,Kim HJ,Padmanabhan K,Swoboda JG,Ahmad I,Kondo T,Gage FH,Theofilopoulos AN,Lawson BR,Schultz PG,Lairson LL

Thu Oct 17 00:00:00 EDT 2013

RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms.

Cell

Fritsch R,de Krijger I,Fritsch K,George R,Reason B,Kumar MS,Diefenbacher M,Stamp G,Downward J

Thu May 23 00:00:00 EDT 2013

Prostacyclin promotes oligodendrocyte precursor recruitment and remyelination after spinal cord demyelination.

Cell death & disease

Takahashi C,Muramatsu R,Fujimura H,Mochizuki H,Yamashita T

Thu Sep 12 00:00:00 EDT 2013

Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.

The Journal of biological chemistry

Steelman AJ,Smith R,Welsh CJ,Li J

Fri Aug 16 00:00:00 EDT 2013

The role of endothelin receptor A during myelination of developing oligodendrocytes.

Journal of Korean medical science

Jung KJ,Kim DW,Lee HN,Lee YS,Lee SJ,Che JH,Lee YH,Kang BC

Sat Jan 01 00:00:00 EST 2011

Novel migrating mouse neural crest cell assay system utilizing P0-Cre/EGFP fluorescent time-lapse imaging.

BMC developmental biology

Kawakami M,Umeda M,Nakagata N,Takeo T,Yamamura K

Wed Nov 09 00:00:00 EST 2011

Bioinformatics

Protein Aliases: H-PDGF-AA; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A

Mouse PDGF-AA Recombinant Protein, PeproTech®

Product Details

315-17-10UG

ELISA (ELISA)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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