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Mouse R-Spondin 1 Recombinant Protein, PeproTech®

Mouse R-Spondin 1 Protein in Functional Assay (Functional)

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Mouse R-Spondin 1 Protein (315-32-20UG) in Functional

Bioassay analysis of Mouse R-Spondin 1 Recombinant Protein, PeproTech® (Product # 315-32-1MG).

Product Image Gallery

Product Details

315-32-20UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 22 publications 22 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Mouse

Published species

Mouse

Expression System

CHO cells

Amino acid sequence

SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCQEGLYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPGSNSR RHKGQQQPQP GTTGPLTSVG PTWAQ

Molecular weight

27 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Murine R-Spondin-1 is a 27.1 kDa protein consisting of 245 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NADK is activated by oncogenic signaling to sustain pancreatic ductal adenocarcinoma.

Cell reports

Schild T,McReynolds MR,Shea C,Low V,Schaffer BE,Asara JM,Piskounova E,Dephoure N,Rabinowitz JD,Gomes AP,Blenis J

Tue Jun 15 00:00:00 EDT 2021

Multiplexed single-cell analysis of organoid signaling networks.

Nature protocols

Sufi J,Qin X,Rodriguez FC,Bu YJ,Vlckova P,Zapatero MR,Nitz M,Tape CJ

Fri Oct 01 00:00:00 EDT 2021

miR-802 regulates Paneth cell function and enterocyte differentiation in the mouse small intestine.

Nature communications

Goga A,Yagabasan B,Herrmanns K,Godbersen S,Silva PN,Denzler R,Zünd M,Furter M,Schwank G,Sunagawa S,Hardt WD,Stoffel M

Mon Jun 07 00:00:00 EDT 2021

High-fat diet-activated fatty acid oxidation mediates intestinal stemness and tumorigenicity.

Cell reports

Mana MD,Hussey AM,Tzouanas CN,Imada S,Barrera Millan Y,Bahceci D,Saiz DR,Webb AT,Lewis CA,Carmeliet P,Mihaylova MM,Shalek AK,Yilmaz ÖH

Tue Jun 08 00:00:00 EDT 2021

Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.

Cell reports

Kubota M,Scheibinger M,Jan TA,Heller S

Tue Jan 19 00:00:00 EST 2021

mTOR signaling regulates gastric epithelial progenitor homeostasis and gastric tumorigenesis via MEK1-ERKs and BMP-Smad1 pathways.

Cell reports

Li K,Wu H,Wang A,Charron J,Mishina Y,Habib SL,Liu H,Li B

Tue May 04 00:00:00 EDT 2021

Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.

Nature communications

Yen YT,Chien M,Wu PY,Ho CC,Ho CT,Huang KC,Chiang SF,Chao KSC,Chen WT,Hung SC

Wed Dec 15 00:00:00 EST 2021

Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage.

Cell reports

Fazilaty H,Brügger MD,Valenta T,Szczerba BM,Berkova L,Doumpas N,Hausmann G,Scharl M,Basler K

Tue Aug 03 00:00:00 EDT 2021

Cell-type-specific signaling networks in heterocellular organoids.

Nature methods

Qin X,Sufi J,Vlckova P,Kyriakidou P,Acton SE,Li VSW,Nitz M,Tape CJ

Sun Mar 01 00:00:00 EST 2020

Radiation mitigation of the intestinal acute radiation injury in mice by 1-[(4-nitrophenyl)sulfonyl]-4-phenylpiperazine.

Stem cells translational medicine

Duhachek-Muggy S,Bhat K,Medina P,Cheng F,He L,Alli C,Saki M,Muthukrishnan SD,Ruffenach G,Eghbali M,Vlashi E,Pajonk F

Wed Jan 01 00:00:00 EST 2020

Regulation of the Paneth cell niche by exogenous L-arginine couples the intestinal stem cell function.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hou Q,Dong Y,Yu Q,Wang B,Le S,Guo Y,Zhang B

Sat Aug 01 00:00:00 EDT 2020

TIGAR/AP-1 axis accelerates the division of Lgr5^- reserve intestinal stem cells to reestablish intestinal architecture after lethal radiation.

Cell death & disease

Chen F,Zhang Y,Hu S,Shi X,Wang Z,Deng Z,Lin L,Zhang J,Pan Y,Bai Y,Liu F,Zhang H,Shao C

Mon Jul 06 00:00:00 EDT 2020

MicroRNA-674-5p induced by HIF-1α targets XBP-1 in intestinal epithelial cell injury during endotoxemia.

Cell death discovery

Liu Z,Jiang J,Dai W,Wei H,Zhang X,Yang Z,Xiong Y

Tue Sep 26 00:00:00 EDT 2023

Nutrient Sensing by the Intestinal Epithelium Orchestrates Mucosal Antimicrobial Defense via Translational Control of Hes1.

Cell host & microbe

Liang S,Guo XK,Ou J,Huang R,Xue Q,Zhang B,Chung Y,Wu W,Dong C,Yang X,Hu X

315-32 was used in Cell Culture to uncover a regulatory circuit that couples host nutrient status to epithelial antimicrobial immunity.

Wed May 08 00:00:00 EDT 2019

Truncated PPM1D impairs stem cell response to genotoxic stress and promotes growth of APC-deficient tumors in the mouse colon.

Cell death & disease

Burocziova M,Burdova K,Martinikova AS,Kasparek P,Kleiblova P,Danielsen SA,Borecka M,Jenikova G,Janečková L,Pavel J,Zemankova P,Schneiderova M,Schwarzova L,Ticha I,Sun XF,Jiraskova K,Liska V,Vodickova L,Vodicka P,Sedlacek R,Kleibl Z,Lothe RA,Korinek V,Macurek L

Mon Oct 28 00:00:00 EDT 2019

Proteomic analysis reveals a protective role of specific macrophage subsets in liver repair.

Scientific reports

Yang W,Zhao X,Tao Y,Wu Y,He F,Tang L

Wed Feb 27 00:00:00 EST 2019

Mesenchyme-free expansion and transplantation of adult alveolar progenitor cells: steps toward cell-based regenerative therapies.

NPJ Regenerative medicine

Weiner AI,Jackson SR,Zhao G,Quansah KK,Farshchian JN,Neupauer KM,Littauer EQ,Paris AJ,Liberti DC,Scott Worthen G,Morrisey EE,Vaughan AE

Tue Sep 29 00:00:00 EDT 2020

Both fallopian tube and ovarian surface epithelium are cells-of-origin for high-grade serous ovarian carcinoma.

Nature communications

Zhang S,Dolgalev I,Zhang T,Ran H,Levine DA,Neel BG

Tue Nov 26 00:00:00 EST 2019

IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance.

Cell host & microbe

Tsai PY,Zhang B,He WQ,Zha JM,Odenwald MA,Singh G,Tamura A,Shen L,Sailer A,Yeruva S,Kuo WT,Fu YX,Tsukita S,Turner JR

315-32 was used in Cell Culture to investigate the pathogenic effects of diarrhea.

Wed Jun 14 00:00:00 EDT 2017

Epithelial Chloride Transport by CFTR Requires TMEM16A.

Scientific reports

Benedetto R,Ousingsawat J,Wanitchakool P,Zhang Y,Holtzman MJ,Amaral M,Rock JR,Schreiber R,Kunzelmann K

Fri Sep 29 00:00:00 EDT 2017

In Vitro Propagation and Branching Morphogenesis from Single Ureteric Bud Cells.

Stem cell reports

Yuri S,Nishikawa M,Yanagawa N,Jo OD,Yanagawa N

Tue Feb 14 00:00:00 EST 2017

Wnt activity and basal niche position sensitize intestinal stem and progenitor cells to DNA damage.

The EMBO journal

Tao S,Tang D,Morita Y,Sperka T,Omrani O,Lechel A,Sakk V,Kraus J,Kestler HA,Kühl M,Rudolph KL

Wed Mar 04 00:00:00 EST 2015

Prostate Sphere-forming Stem Cells Are Derived from the P63-expressing Basal Compartment.

The Journal of biological chemistry

Huang Y,Hamana T,Liu J,Wang C,An L,You P,Chang JYF,Xu J,McKeehan WL,Wang F

Fri Jul 17 00:00:00 EDT 2015

Tumor cell-specific inhibition of MYC function using small molecule inhibitors of the HUWE1 ubiquitin ligase.

EMBO molecular medicine

Peter S,Bultinck J,Myant K,Jaenicke LA,Walz S,Müller J,Gmachl M,Treu M,Boehmelt G,Ade CP,Schmitz W,Wiegering A,Otto C,Popov N,Sansom O,Kraut N,Eilers M

Mon Dec 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Cristin-3; Cysteine-rich and single thrombospondin domain-containing protein 3; FLJ40906; mCristin-3; R-spondin-1; Roof plate-specific spondin-1; RP11-566C13.1; thrombospondin type 1 domain containing gene Rspondin

Mouse R-Spondin 1 Recombinant Protein, PeproTech®

Mouse R-Spondin 1 Protein (315-32-20UG) in Functional

Product Details

315-32-20UG

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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