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Invitrogen
Mouse VEGFR3 (Flt-4) Fc Chimera Recombinant Protein
Product Details
RP-88261
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse FLT4, amino acids Met1-Glu775 (Accession # P35917-1) with a C-terminal human IgG1 Fc region
Tag
Fc-tag
Molecular weight
112 kDa
Class
Recombinant
Type
Protein
Purity
>92% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
1. Measured by its ability to bind human VEGF-D in functional ELISA. 2. Immobilized human VEGF-C at 10 µg/mL (100 µL/well) can bind mouse VEGFR3-Fc. The EC50 of mouse VEGFR3-Fc is 0.008 µg/mL. 3. Measured by its ability to bind with mouse FIGF-His in a functional ELISA.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
Vascular endothelial growth factor is a key regulator of blood vessel development in embryos and angiogenesis in adult tissues. Unlike VEGF, the related VEGFC stimulates the growth of lymphatic vessels through its specificl ymphatic endothelial receptor VEGFR-3. In 1998, Dumont showed that targeted inactivation of the VEGFR-3 gene in mice resulted in defective blood vessel development in early embryos. Vasculogenesis and angiogenesis occurred, but large vessels became abnormally organized with defective lumens, leading to fluid accumulation in the pericardial cavity and cardiovascular failure at embryonic day 9.5. Thus, VEGFR3 has an essential role in the development of theembryonic cardiovascular system before the emergence of the lymphatic vessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chylous ascites; FLT-4; Fms-like tyrosine kinase 4; receptor protein tyrosine kinase; soluble VEGFR 3; sVEGFR 3; Tyrosine-protein kinase receptor FLT4; Vascular endothelial growth factor receptor 3; vascular endothelial growth factor receptor-3; VEGF R3; VEGFR-3
Gene Aliases: AI323512; Chy; Flt-4; Flt4; VEGFR-3; VEGFR3
UniProt ID: (Mouse) P35917
Entrez Gene ID: (Mouse) 14257
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