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PeproGMP® Human EGF Recombinant Protein, PeproTech®

PeproGMP® Human EGF Protein in Functional Assay (Functional)

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PeproGMP® Human EGF Protein (GMP100-15-100UG) in Functional

Cell proliferation assay analysis of PeproGMP® Human EGF Recombinant Protein, PeproTech (Product # GMP100-15-100UG) compared to Human EGF, Animal-Free Recombinant Protein, PeproTech (Product # AF-100-15-1MG), demonstrating comparable bioactivity across product grades.

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Product Details

GMP100-15-100UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human

Expression System

E. coli

Amino acid sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Molecular weight

6.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<0.1 EU/µg

Activity

Determined by the proliferation of mouse Balb/c 3T3 cells. PeproGMP Recombinant Human EGF has been tested against the WHO standard NIBSC 91/530. Find the International Unit (IU) value here.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Additional product information and specifications are provided on the Certificate of Analysis.

All PeproGMP® products are manufactured using animal-derived component free materials.

Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.

Product use statement: For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Light and electron microscopy continuum-resolution imaging of 3D cell cultures.

Developmental cell

D'Imprima E,Garcia Montero M,Gawrzak S,Ronchi P,Zagoriy I,Schwab Y,Jechlinger M,Mahamid J

GMP100-15 was used in Cell Culture to demonstrate this workflow on mouse and human 3D cultures and use automated image segmentation to annotate and quantitatively analyze subcellular structures in patient-derived colorectal cancer organoids.

Mon Apr 10 00:00:00 EDT 2023

Programming inactive RNA-binding small molecules into bioactive degraders.

Nature

Tong Y,Lee Y,Liu X,Childs-Disney JL,Suresh BM,Benhamou RI,Yang C,Li W,Costales MG,Haniff HS,Sievers S,Abegg D,Wegner T,Paulisch TO,Lekah E,Grefe M,Crynen G,Van Meter M,Wang T,Gibaut QMR,Cleveland JL,Adibekian A,Glorius F,Waldmann H,Disney MD

GMP100-15 was used in Cell Culture to provide a proof of concept, designing selective degraders for the precursor to the disease-associated microRNA-155 (pre-miR-155), JUN mRNA and MYC mRNA.

Thu Jun 01 00:00:00 EDT 2023

Architectural control of metabolic plasticity in epithelial cancer cells.

Communications biology

Al-Masri M,Paliotti K,Tran R,Halaoui R,Lelarge V,Chatterjee S,Wang LT,Moraes C,McCaffrey L

Fri Mar 19 00:00:00 EDT 2021

Bromodomain inhibitor jq1 induces cell cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway.

International journal of oncology

Wen N,Guo B,Zheng H,Xu L,Liang H,Wang Q,Wang D,Chen X,Zhang S,Li Y,Zhang L

GMP100-15 was used in Cell Culture to explore the underlying mechanisms of the anti-tumour effects of Brd4 and the bromodomain inhibitor JQ1 on glioma stem cells.

Tue Oct 01 00:00:00 EDT 2019

Endoplasmic reticulum stress disrupts lysosomal homeostasis and induces blockade of autophagic flux in human trophoblasts.

Scientific reports

Nakashima A,Cheng SB,Kusabiraki T,Motomura K,Aoki A,Ushijima A,Ono Y,Tsuda S,Shima T,Yoshino O,Sago H,Matsumoto K,Sharma S,Saito S

GMP100-15 was used in Cell Culture to show that ER stress reduces the number of lysosomes, resulting in inhibition of autophagic flux in trophoblast cells.