Gibco

Rat EGF Recombinant Protein, PeproTech®

Rat EGF Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Rat EGF Protein (400-25-100UG) in Functional

Bioassay analysis of Rat EGF Recombinant Protein, PeproTech® (Product # 400-25-1MG).

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Product Details

400-25-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 24 publications 24 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species

Rat

Published species

Human, Rat

Expression System

E. coli

Amino acid sequence

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Molecular weight

6.2 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Rat EGF is a 6.2 kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide bonds.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Autophagy Is Required for Hepatic Differentiation of Hepatic Progenitor Cells via Wnt Signaling Pathway.

BioMed research international

Zeng J,Jing Y,Wu Q,Zeng J,Wei L,Liu J

Tue May 25 00:00:00 EDT 2021

Autophagy Is Required for Hepatic Differentiation of Hepatic Progenitor Cells via Wnt Signaling Pathway.

BioMed research international

Zeng J,Jing Y,Wu Q,Zeng J,Wei L,Liu J

Tue May 25 00:00:00 EDT 2021

Kinetics of receptor tyrosine kinase activation define ERK signaling dynamics.

Science signaling

Kiyatkin A,van Alderwerelt van Rosenburgh IK,Klein DE,Lemmon MA

400-25 was used in In vitro experiments to investigate if receptor tyrosine kinases activation kinetics play a crucial role in determining MAP kinase cascade signaling dynamics and cell fate decisions, and if signaling outcome can be modified by activating a given receptor tyrosine kinases in different ways.

Tue Aug 18 00:00:00 EDT 2020

Transplantation of Nurr1-overexpressing neural stem cells and microglia for treating parkinsonian rats.

CNS neuroscience & therapeutics

Qian Y,Chen XX,Wang W,Li JJ,Wang XP,Tang ZW,Xu JT,Lin H,Yang ZY,Li LY,Song XB,Guo JZ,Bian LG,Zhou L,Lu D,Deng XL

Wed Jan 01 00:00:00 EST 2020

Wnt4-modified NSC transplantation promotes functional recovery after spinal cord injury.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li X,Peng Z,Long L,Tuo Y,Wang L,Zhao X,Le W,Wan Y

Wed Jan 01 00:00:00 EST 2020

A modified protocol for the isolation, culture, and cryopreservation of rat embryonic neural stem cells.

Experimental and therapeutic medicine

Zhou H,Yang H,Lu L,Li X,Pan B,Fu Z,Chu T,Liu J,Kang Y,Liu L,Ning G,Ding W,Wu P,Kong X,Feng S

400-25 was used in Sample Preparation to provide an updated, successful and superiorly-efficient protocol for isolation, culture and cryopreservation of rat embryonic NSCs.

Tue Dec 01 00:00:00 EST 2020

Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns.

Scientific reports

Park GW,Heo J,Kang JY,Yang JW,Kim JS,Kwon KD,Yu BC,Lee SJ

Fri Jul 24 00:00:00 EDT 2020

Regeneration competent satellite cell niches in rat engineered skeletal muscle.

FASEB bioAdvances

Tiburcy M,Markov A,Kraemer LK,Christalla P,Rave-Fraenk M,Fischer HJ,Reichardt HM,Zimmermann WH

Sun Dec 01 00:00:00 EST 2019

MMP-sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC-mediated angiogenesis.

Experimental and therapeutic medicine

Ouyang L,Dan Y,Shao Z,Yang S,Yang C,Liu G,Duan D

400-25 was used in In vitro experiments to provide a potential novel treatment approach for soft tissue defects such as bone exposure, chronic skin ulcers, bedsores, limb necrosis, osteonecrosis and other ischemic diseases.

Tue Oct 01 00:00:00 EDT 2019

Kidney-derived c-kit⁺ progenitor/stem cells contribute to podocyte recovery in a model of acute proteinuria.

Scientific reports

Rangel EB,Gomes SA,Kanashiro-Takeuchi R,Saltzman RG,Wei C,Ruiz P,Reiser J,Hare JM

Wed Oct 03 00:00:00 EDT 2018

Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis.

Oncogene

Li J,Hu SB,Wang LY,Zhang X,Zhou X,Yang B,Li JH,Xiong J,Liu N,Li Y,Wu YZ,Zheng QC

Thu Nov 30 00:00:00 EST 2017

Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis.

Oncogene

Li J,Hu SB,Wang LY,Zhang X,Zhou X,Yang B,Li JH,Xiong J,Liu N,Li Y,Wu YZ,Zheng QC

Thu Nov 30 00:00:00 EST 2017

CCAAT/Enhancer binding protein β silencing mitigates glial activation and neurodegeneration in a rat model of Parkinson's disease.

Scientific reports

Morales-Garcia JA,Gine E,Hernandez-Encinas E,Aguilar-Morante D,Sierra-Magro A,Sanz-SanCristobal M,Alonso-Gil S,Sanchez-Lanzas R,Castaño JG,Santos A,Perez-Castillo A

Thu Oct 19 00:00:00 EDT 2017

Reprogramming of Pancreatic Exocrine Cells AR42J Into Insulin-producing Cells Using mRNAs for Pdx1, Ngn3, and MafA Transcription Factors.

Molecular therapy. Nucleic acids

Koblas T,Leontovyc I,Loukotova S,Kosinova L,Saudek F

Tue May 17 00:00:00 EDT 2016

The distribution and adipogenic potential of perivascular adipose tissue adipocyte progenitors is dependent on sexual dimorphism and vessel location.

Physiological reports

Contreras GA,Thelen K,Ayala-Lopez N,Watts SW

400-25 was used in Cell Culture to test the hypothesis that vessel location and sexual dimorphism affect the distribution and adipogenic capacity of APC in cardiovascular disease risk relevant PVAT sites.

Sat Oct 01 00:00:00 EDT 2016

Generation and characterization of rat liver stem cell lines and their engraftment in a rat model of liver failure.

Scientific reports

Kuijk EW,Rasmussen S,Blokzijl F,Huch M,Gehart H,Toonen P,Begthel H,Clevers H,Geurts AM,Cuppen E

Fri Feb 26 00:00:00 EST 2016

Preventive cancer stem cell-based vaccination reduces liver metastasis development in a rat colon carcinoma syngeneic model.

Stem cells (Dayton, Ohio)

Duarte S,Momier D,Baqué P,Casanova V,Loubat A,Samson M,Guigonis JM,Staccini P,Saint-Paul MC,De Lima MP,Carle GF,Pierrefite-Carle V

Fri Mar 01 00:00:00 EST 2013

Neural and oligodendrocyte progenitor cells: transferrin effects on cell proliferation.

ASN neuro

Silvestroff L,Franco PG,Pasquini JM

Fri Mar 08 00:00:00 EST 2013

Energy budget, behavior and leptin in striped hamsters subjected to food restriction and refeeding.

PloS one

Zhao ZJ,Zhu QX,Chen KX,Wang YK,Cao J

Mon Jul 22 00:00:00 EDT 2013

Minocycline-preconditioned neural stem cells enhance neuroprotection after ischemic stroke in rats.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sakata H,Niizuma K,Yoshioka H,Kim GS,Jung JE,Katsu M,Narasimhan P,Maier CM,Nishiyama Y,Chan PH

400-25 was used in Cell Culture to investigate whether reprogramming neural stem cells with minocycline enhances survival of grafted cells and promotes neuroprotection in ischemic stroke.

Wed Mar 07 00:00:00 EST 2012

Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke.

Journal of translational medicine

Sun C,Zhang H,Li J,Huang H,Cheng H,Wang Y,Li P,An Y

Fri Aug 20 00:00:00 EDT 2010

Interferon-gamma produced by microglia and the neuropeptide PACAP have opposite effects on the viability of neural progenitor cells.

PloS one

Mäkelä J,Koivuniemi R,Korhonen L,Lindholm D

Mon Jun 14 00:00:00 EDT 2010

Murine "cardiospheres" are not a source of stem cells with cardiomyogenic potential.

Stem cells (Dayton, Ohio)

Andersen DC,Andersen P,Schneider M,Jensen HB,Sheikh SP

Wed Jul 01 00:00:00 EDT 2009

Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation.

American journal of physiology. Cell physiology

Tatsumi R,Sankoda Y,Anderson JE,Sato Y,Mizunoya W,Shimizu N,Suzuki T,Yamada M,Rhoads RP,Ikeuchi Y,Allen RE

400-25 was used in Sample Preparation (PCR) to investigate the expression of a neural secreted chemorepellent semaphorin 3A on satellite cells regeneration and muscle fiber growth.