Gibco

Rat GDNF Recombinant Protein, PeproTech®

Rat GDNF Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Rat GDNF Protein (450-51-10UG) in Functional

Bioassay analysis of Rat GDNF Recombinant Protein, PeproTech® (Product # 450-51-1MG).

Product Image Gallery

Product Details

450-51-10UG

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Rat

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI

Molecular weight

30 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to stimulate the proliferation of rat C6 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

The functional rat GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Rat GDNF is 30.0 kDa.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MiR34 contributes to spinal muscular atrophy and AAV9-mediated delivery of MiR34a ameliorates the motor deficits in SMA mice.

Molecular therapy. Nucleic acids

Chen TH,Chang SH,Wu YF,Yen YP,Hsu FY,Chen YC,Ming Y,Hsu HC,Su YC,Wong ST,Hung JH,Chiou SH,Jong YJ,Chen JA

450-51 was used in In vitro experiments to propose that MiR34 may serve as a biomarker of SMA since it is associated with the pathology and can help evaluate the therapeutic effects of nusinersen.

Tue Jun 13 00:00:00 EDT 2023

Oncostatin M induces hyperalgesic priming and amplifies signaling of cAMP to ERK by RapGEF2 and PKA.

Journal of neurochemistry

Garza Carbajal A,Ebersberger A,Thiel A,Ferrari L,Acuna J,Brosig S,Isensee J,Moeller K,Siobal M,Rose-John S,Levine J,Schaible HG,Hucho T

450-51 was used in Sample Preparation to conclude that priming induced by OSM uses a novel mechanism to enhance and prolong coupling of cAMP/PKA to ERK1/2 signaling without changing the overall pathway structure.

Tue Jun 01 00:00:00 EDT 2021

A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.

Cell reports

Nakagawa T,Jörg DJ,Watanabe H,Mizuno S,Han S,Ikeda T,Omatsu Y,Nishimura K,Fujita M,Takahashi S,Kondoh G,Simons BD,Yoshida S,Nagasawa T

Tue Oct 19 00:00:00 EDT 2021

AKT3 Is a Pivotal Molecule of Cadherin-22 and GDNF Family Receptor-α1 Signal Pathways Regulating Self-Renewal in Female Germline Stem Cells.

Stem cells (Dayton, Ohio)

Zhang X,Wei R,Sun Y,Xia Q,Xie W,Song H,Wang W,Zou K

Thu Aug 01 00:00:00 EDT 2019

α-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson's disease.

Nature communications

Ludtmann MHR,Angelova PR,Horrocks MH,Choi ML,Rodrigues M,Baev AY,Berezhnov AV,Yao Z,Little D,Banushi B,Al-Menhali AS,Ranasinghe RT,Whiten DR,Yapom R,Dolt KS,Devine MJ,Gissen P,Kunath T,Jaganjac M,Pavlov EV,Klenerman D,Abramov AY,Gandhi S

Tue Jun 12 00:00:00 EDT 2018

Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice.

Stem cell reports

Shinohara T,Kazuki K,Ogonuki N,Morimoto H,Matoba S,Hiramatsu K,Honma K,Suzuki T,Hara T,Ogura A,Oshimura M,Kanatsu-Shinohara M,Kazuki Y

Tue Oct 10 00:00:00 EDT 2017

Somatodendritic Expression of JAM2 Inhibits Oligodendrocyte Myelination.

Neuron

Redmond SA,Mei F,Eshed-Eisenbach Y,Osso LA,Leshkowitz D,Shen YA,Kay JN,Aurrand-Lions M,Lyons DA,Peles E,Chan JR

Wed Aug 17 00:00:00 EDT 2016

Somatodendritic Expression of JAM2 Inhibits Oligodendrocyte Myelination.

Neuron

Redmond SA,Mei F,Eshed-Eisenbach Y,Osso LA,Leshkowitz D,Shen YA,Kay JN,Aurrand-Lions M,Lyons DA,Peles E,Chan JR

Wed Aug 17 00:00:00 EDT 2016

MicroRNA-7 Promotes Glycolysis to Protect against 1-Methyl-4-phenylpyridinium-induced Cell Death.

The Journal of biological chemistry

Chaudhuri AD,Kabaria S,Choi DC,Mouradian MM,Junn E

Fri May 08 00:00:00 EDT 2015

Th2 Cytokines Augment IL-31/IL-31RA Interactions via STAT6-dependent IL-31RA Expression.

The Journal of biological chemistry

Edukulla R,Singh B,Jegga AG,Sontake V,Dillon SR,Madala SK

Fri May 22 00:00:00 EDT 2015

Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal.

Proceedings of the National Academy of Sciences of the United States of America

Kanatsu-Shinohara M,Onoyama I,Nakayama KI,Shinohara T

Tue Jun 17 00:00:00 EDT 2014

Identification of a key motif that determines the differential surface levels of RET and TrkB tyrosine kinase receptors and controls depolarization enhanced RET surface insertion.

The Journal of biological chemistry

Li XZ,Yan J,Huang SH,Zhao L,Wang J,Chen ZY

Fri Jan 13 00:00:00 EST 2012

3D multicolor super-resolution imaging offers improved accuracy in neuron tracing.

PloS one

Lakadamyali M,Babcock H,Bates M,Zhuang X,Lichtman J

Mon Jun 04 00:00:00 EDT 2012

Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in the mouse testis.

PloS one

Kanatsu-Shinohara M,Takashima S,Ishii K,Shinohara T

Wed Feb 15 00:00:00 EST 2012

Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes.

PloS one

Sato T,Aiyama Y,Ishii-Inagaki M,Hara K,Tsunekawa N,Harikae K,Uemura-Kamata M,Shinomura M,Zhu XB,Maeda S,Kuwahara-Otani S,Kudo A,Kawakami H,Kanai-Azuma M,Fujiwara M,Miyamae Y,Yoshida S,Seki M,Kurohmaru M,Kanai Y

Mon Apr 23 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Astrocyte-derived trophic factor; ATF; glial cell line derived neurotrophic factor; Glial cell line derived neutrophic factor; Glial cell line-derived neurotrophic factor; H-GDNF

Rat GDNF Recombinant Protein, PeproTech®

Rat GDNF Protein (450-51-10UG) in Functional

Product Details

450-51-10UG

Western Blot (WB)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species

Published species

Expression System

Amino acid sequence

Molecular weight

Class

Type

Purity

Endotoxin concentration

Activity

Conjugate

Form

Purification

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

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