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Product Details
RRIL2I
Applications
Tested Dilution
Publications
Product Specifications
Species
Rat
Expression System
P. pastoris
Amino acid sequence
Rat IL-2, amino acids 21-155 (Accession # P04351)
Molecular weight
15.5 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Activity
ED50 = 3 ng/mL; determined by the dose-dependent proliferation of mouse CTLL-2 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
Mature rat IL-2 was expressed in Pichia pastoris, X-33. GenBank Accession # P04351, amino acids 21 - 155. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: APTSSPAKET. Purity is >95% by SDS-PAGE analyses.
Activity: The biological activity of recombinant rat IL-2 was determined by measuring the proliferation of mouse CTLL-2 cells. The ED50 (or 1 unit of activity) for this effect is typically 3 ng/mL.
Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.
Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF
Gene Aliases: Il-2; Il2
UniProt ID: (Rat) P17108
Entrez Gene ID: (Rat) 116562
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