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Invitrogen
SARS-CoV-2 Spike Protein (RBD) (aa319-541), mFc Tag Recombinant Protein
FIGURE: 1 / 4
SARS-CoV-2 Spike Protein (RBD) (aa319-541), mFc Tag Protein (RP-87700) in ELISA
ELISA analysis of immobilized Recombinant Human ACE2 Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike RBD-mFc Protein (Product # RP-87700). The EC50 of Recombinant SARS-COV-2 Spike RBD-mFc Protein is 2.58 ng/mL.
Product Details
RP-87700
Applications
Tested Dilution
Publications
Product Specifications
Species
Virus
Expression System
HEK293 cells
Amino acid sequence
SARS-CoV-2 Spike Protein RBD, amino acids Arg319-Phe541 with a C-terminal mFc-tag
Tag
Fc-tag
Molecular weight
50.8 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.1 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2 µg/mL (100 µL/well) can bind Recombinant SARS-COV-2 Spike RBD-mFc Protein, the ED50 of Recombinant SARS-COV-2 Spike RBD-mFc Protein is 2.58 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
Product Specific Information
Animal Origin Free
Storage and Reconstitution: Store the lyophilized protein at -20°C to -80°C for long term. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2
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