Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
SARS-CoV-2 Spike Protein S1/S2 (B.1.618) His-tag Recombinant Protein
Product Details
RP-88548
Product Specifications
Species
Virus
Expression System
Baculovirus
Amino acid sequence
SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1), amino acids Met1-Pro1213 with YH145-146 deletion, E484K, D614G substitutions and a C-terminal His-tag
Tag
His-tag
Molecular weight
134.03 kDa
Class
Recombinant
Type
Protein
Purity
>95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 Spike S1+S2 (YH145-146 deletion, E484K, D614G) Protein (ECD, His Tag), the EC50 of SARS-CoV-2 Spike S1+S2 (YH145-146 deletion, E484K, D614G) Protein (ECD, His Tag) is 100-700 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
20mM tris, pH 8, with <0.1µg/mL BSA, 10% glycerol, 0.01% mannitol, 300mM NaCl, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
The mutations were identified in the SARS-CoV-2 varient known as B.1.618
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. SARS-CoV-2 S is 1273 amino acids long and consists of a signal peptide (amino acids 1-13) located at the N-terminus, the S1 subunit (14-685 residues), and the S2 subunit (686-1273 residues); the last two regions are responsible for receptor binding and membrane fusion, respectively. The S-ECD is the extracellular domain of the Spike protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support