Preparative HPLC Columns

Overview

Seamlessly scale up your analytical method for robust purification

With preparative columns, one size does not fit all. Whether you need unique chemistries for difficult separations to purify your compound of interest, robust columns for longer use, or budget-friendly solutions for routine applications, our preparative HPLC column portfolio has something to meet your needs. Our full range of customizable column hardware with optional XtendedLife technology offers robust, reproducible performance and expanded column capabilities in a wide variety of stationary phase chemistries, making it easy to scale up from your current analytical method.

Experience expanded column capabilities from our complete preparative HPLC column portfolio
Robust, customizable solutions to meet any application needs
Scale-up from your current analytical method with ease

All HPLC and UHPLC Columns

Thermo Scientific preparative HPLC columns are available in 40+ stationary phase chemistries, allowing for maximum flexibility and ensuring that you have options to scale up your analytical workflows with chemistry continuity for easy method transfer.

Our preparative stationary phases are offered in three families:

Popular Thermo Scientific phases	We have expanded our current offering of popular Thermo Scientific column chemistries, allowing customers to seamlessly scale up their current analytical methods. Meet the most challenging separations with chemistries that are manufactured to the highest standards and provide excellent selectivity and separation for a variety of applications. Available column chemistries include Hypersil GOLD, Accucore, MAbPac, and DNAPac.
Hypersil PREP phases	An excellent choices for routine applications that require development from analytical to preparative HPLC scale. Available in C18, C8, silica, ODS, BDS, and phenyl phases, the Hypersil PREP range has been designed to provide good selectivity for a wide range of applications.
Hypersil PREP HS phases	Our PREP HS line features high surface area particles with a 100 Å pore size to maximize sample retention and loading. An excellent choice for small molecule and drug purification applications, Hypersil PREP HS is available in C18, C8, silicone, and phenyl phases.

If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in preparative scale or would like to discuss a specific phase that is not listed above, we would be happy to discuss your requirements. Please contact your local sales representative for customer specific orders. Please note that custom built columns are not eligible for returns.

Popular Thermo Scientific stationary phase details

	Pore size (Å)	Surface area (m²/g)	Carbon load (%)	USP code	pH range
Hypersil GOLD C18	175	220	10	L1	2 to 9
Hypersil GOLD C8	175	220	8	L7	2 to 9
Hypersil GOLD aQ	175	220	12	L1	2 to 9
Hypersil GOLD C4	175	220	5	L26	2 to 8
Hypersil GOLD Phenyl	175	220	8	L11	2 to 8
Hypercarb	250	120	100	L109	0 to 14
Syncronis C18	100	320	16	L1	2 to 9
Syncronis HILIC	100	320	5	None	2 to 8
Accucore XL C18	80	90	7	L1	1 to 11
Accucore XL C8	80	90	4	L7	2 to 9
DNAPac RP	Proprietary wide pore	22-24	N/A	None	0 to 14
DNAPac PA200	Nonporous	N/A	N/A	None	4 to 10
MAbPac	Nonporous	N/A	N/A	None	2 to 12
ProPac	Nonporous	N/A	N/A	None	2 to 12

Hypersil PREP stationary phase details

	Pore size (Å)	Surface area (m²/g)	Carbon load (%)	USP code	pH range
Hypersil PREP C18 BDS	145	185	11	L1	2 to 8
Hypersil PREP C8 BDS	145	185	7	L7	2 to 8
Hypersil PREP ODS	130	195	10	L1	2 to 8
Hypersil PREP Si BDS	145	185	0	L3	2 to 7
Hypersil PREP Si	130	195	0	L3	2 to 7
Hypersil PREP Phenyl BDS	145	185	5	L11	2 to 8

Hypersil PREP HS stationary phase details

	Pore size (Å)	Surface area (m²/g)	Carbon load (%)	USP code	pH range
Hypersil PREP C18 HS	100	350	15	L1	2 to 9
Hypersil PREP C8 HS	100	350	7	L7	2 to 8
Hypersil PREP Si HS	100	350	0	L3	2 to 7
Hypersil PREP Phenyl HS	100	350	9	L11	2 to 8

Preparative column hardware

We offer three column hardware options based on column inner diameter (I.D.) and column performance needs:

Standard threaded and standard flange hardware

Expertly packed and robustly designed, standard hardware balances cost and lifetime to provide versatile products for routine use.
Made of high quality stainless steel designed for standard applications and available in all sizes up to 200mm i.d.
Standard threaded hardware for column i.d. < 40mm, and standard flange hardware for column i.d. ≥ 40mm.
Standard hardware can be modified on request to be compatible with supercritical fluid chromatography.

XtendedLife hardware

Improves column lifetime by incorporating an internal Dynamic Axial Compression system, ensuring the media bed is continuously packed tightly.
Compatible with supercritical fluid chromatography and available in dimensions up to 50mm I.D.

Preparative HPLC column method transfer tool

Use this online tool to determine best starting conditions for scaling methods between analytical to preparative HPLC conditions. The tool will calculate the new injection volume, flow rate, gradient table and expected eluent usage. Just insert your current method conditions and your new column dimensions to get up and running faster.

Quickly scale methods from analytical to preparative HPLC ›

Resources

2022/2023 Chromatography Columns and Consumables Catalog

Your complete, interactive, digital guide to all Thermo Scientific chromatography columns and consumables products. Our enhanced online 2022/2023 catalog features easy access to product information, key application notes, and brand-new selector tools.

Download consumables catalog ›