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We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios.
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Here are possible causes and solutions:
We would recommend checking to make sure that the gene of interest is cloned in-frame with the Platelet-derived Growth Factor Receptor (PDGFR) transmembrane domain.
If the gene of interest has a start codon in the context of a perfect Kozak sequence, it maybe preferentially translated over the vector’s initiation codon, resulting in no leader sequence and no glycosylation, and hence no targeting to the endoplasmic reticulum and no secretion. This is rare, but it has been observed. If it is a problem, we recommend using PCR to delete the start codon.
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