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Choose from a menu of verified real-time PCR research assays to build your own custom Applied Biosystems TaqMan SARS-CoV-2 Mutation Panel, and get the results you need today, while preparing you for what’s to come. This scalable solution lets you run a few or hundreds of samples to identify one or many mutations—all on your current real-time PCR instrumentation.
For Research Use Only. Not for use in diagnostic procedures.
Targets | Choose from a menu of verified real-time PCR SNP research assays to build your custom panel |
Assay design | Sequence-specific forward and reverse primers to amplify the target sequence region. The reverse primer also primes reverse transcription of the SARS-CoV-2 genomic RNA sequences. Each assay includes two TaqMan minor groove binder (MGB) probes with nonfluorescent quenchers (NFQ): |
Available sizes | 375 reactions 1,250 reactions |
Sample input | RNA extracted from SARS-CoV-2 samples with a CT value of less or equal to 30 |
Turnaround time | 1 hour and 10 min from extracted RNA to results |
Recommended instruments | Any real-time PCR instrument such as the Applied Biosystems 7500, 7500 Fast, QuantStudio 5, and QuantStudio 7 systems |
Recommended software | QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module |
Our menu of verified real-time PCR research assays provide robust detection of SARS-CoV-2 mutations. Below are results (cluster plots) as viewed using QuantStudio Design and Analysis Software v2.5 with the Genotyping Analysis Module. These cluster plots show very clear discrimination between the wild type samples (red dots along the x-axis) and the mutation samples (blue dots along the y-axis).
Figure 2. S gene mutation delH69V70 cluster plot. delH69V70 (also known as 69-70del) is a known mutation of B.1.1.7 (501Y.V1 or UK variant). delH69V70 is not exclusive to B.1.1.7.
Starting with isolated RNA from SARS-CoV-2 positive samples, our unique workflow combines our gold-standard Applied Biosystems TaqMan SNP Genotyping Assays with a one-step RT-PCR reaction to detect whether there are known mutations present in your samples or not.
In this video, we’ll show you how this one-step RT-PCR reaction works and what you can expect your results to look like.
In this short, two-video tutorial, you will learn how to:
Go to thermofisher.com/educationconnect and log into your account or create a new one!
Tips and tricks for setting up your experiment and analyzing results
In this short video, you will learn:
Use QuantStudio Design and Analysis Software v2.5 or later with the Genotyping Analysis Module.
To run the TaqMan SARS-CoV-2 Mutation Panel, you will need to order following:
Naming Convention: Gene.Mutation.Reference Codon.Mutant Codon
Example: S.D215G.GAT.GGT
Note: For multi-nucleotide deletions, the reference codon and mutant codon are not part of the naming convention. (Example: S.delH69V70)
Assays associated with the publication, A method for variant agnostic detection of SARS-CoV-2, rapid monitoring of circulating variants, detection of mutations of biological significance, and early detection of emergent variants such as Omicron by E. Lai, D. Becker, et al. are highlighted below in blue.
Need help selecting assays to detect variants of concern? Learn more here.
For additional assistance on mutation assay selection, please contact us.
NIH-funded project offers efficient approach when tracking SARS-CoV-2 variants
The assays highlighted in grey are the current assays used in the Rosalind Dashboard.
*Recommend running an additional assay in parallel with V213G, such as Q493R, that will produce amplification from BA.1 which is found to a high prevalence in Omicron variants.
Interested in GeneArt strings? Contact us to discuss options.
Interested in GeneArt strings? Contact us to discuss options.
For Research Use Only. Not for use in diagnostic procedures.