Versatile solutions for human leukocyte antigen (HLA) testing with peripheral blood

Next-generation sequencing (NGS) technologies transformed the HLA typing field by generating high-resolution data and resolving most allelic ambiguities without employing multiple reflexive tests. However, high-performance NGS HLA assays demand high-quality genomic DNA. The DNA can be sourced from biological samples such as peripheral blood.

This webinar presents the performance of 96 DNA samples extracted from whole blood using the Thermo Scientific MagMAX magnetic bead-based kit. Analysis was performed on the KingFisher Flex system for high-resolution HLA typing with the Thermo Scientific One Lambda AllType and AllType FASTplex NGS kits.

This webinar also highlights how high-quality DNA positively impacts the typing process, and it highlights the crucial importance of quality checks as executed with Thermo Scientific NanoDrop Microvolume UV-Vis Spectrophotometers on the NGS workflow.

Watch this webinar to gain a broad understanding of this assay technique:

• Understand the benefits of DNA extraction utilizing magnetic beads, as performed on the MagMAX magnetic bead-based kit on the KingFisher Apex system.
  
  
• Learn about applying quality control checks on extracted DNA using absorbance measurements from NanoDrop Microvolume Spectrophotometers, and see how these measurements ensure successful downstream NGS applications.
  
  
• Review a case study evaluating the MagMAX / KingFisher DNA purification system for high-resolution HLA typing.