Resources for the Tali Image Cytometer | Thermo Fisher Scientific

Support for your Tali instrument

The Invitrogen Tali Image-based Cytometer has been discontinued, but you can still find consumables and accessories, software download information, and compatible reagents, as well as videos and application notes for your instrument (see below).

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Tali Image-based Cytometer resources

Product manual

Tali Image-Based Cytometer product manual

Application notes

Citations

The Tali Image-Based Cytometer can be used to detect green and red fluorescence of a variety of dyes and stains. This table ranks the fluorescence intensity of various dyes when examined on the Tali Image-Based Cytometer. Unless otherwise indicated, cells were labeled with the indicated fluorescent dye or stain at concentrations suggested in the product manual and analyzed on the Tali Image-Based Cytometer using the “Green+Red Setting” setting. In some cases, there was spectral overlap of the fluorescence emission (commonly known as bleedthrough) into the opposite channel. This information is also included in the table.

Red channel dyes

Dye, product tested	Excitation (nm)	Emission (nm)	Expected emission channel	Green channel signal	Red channel signal	Recommendations
7-AAD (7-aminoactinomycin D)	549	645	Red	None	+++	Use 1–2 uL per mL
R-PE (R-phycoerythrin), CD3-RPE conjugate	564	577	Red	None	++	Test titration of antibody conjugate
CellROX™ Orange Reagent	545	565	Red	None	+++	Use according to product manual
MitoTracker™ Orange CM-H2TMRos	551	575	Red	None	++	Use according to product manual
Ethidium homodimer-1	528	618	Red	None	+++	Use 1 uM
Rhodamine Red-X anti-mouse secondary	573	591	Red	None	+	Basically undetectable

Green channel dyes

Dye, product tested	Excitation (nm)	Emission (nm)	Expected emission channel	Green channel signal	Red channel signal	Recommendations
SYTOX™ Green stain	504	523	Green	+++	+	Use 12.5 nM
LIVE/DEAD™ Fixable Green Dead Cell Stain	495	520	Green	+++	++	Spectral overlap into red channel at lowest concentration tested (0.25 µL/mL)
CellTrace™ Green	492	517	Green	+++	+	Use at 50 nM–250 nM
CellEvent™ Green reagent	502	530	Green	++	None	10 µM final concentration
SYTO™ 9 stain	485	498/501	Green	+++	None	Use 100 nM
TO-PRO™-1	515	531	Green	++	++	Due to spectral overlap can not be used for multiplexing experiments
FM™ 1-43X	510	626	Green	+++	+++	Due to spectral overlap can not be used for multiplexing experiments
CellTracker™ Green CFDA-AM	492	517	Green	+++	None	Use according to product manual
5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA), mixed isomers	505	525	Green	++	None	Use at 25–50 uM
Annexin V-FITC	494	518	Green	++	None	Use according to product manual (test 10 uL Annexin V-FITC if signal is dim)
Acridine Orange	500	526	Green	+++	None	For Jurkat cells suggest using 0.25 – 0.5 uM (test titration of the dye to ensure no overlap into the red channel)
Calcein AM	494	517	Green	+++	+	Use at 0.1–1 uM (test titration of dye to ensure no overlap into red channel).

+ = Undetectable to barely detectable on the histogram, but can see spots on the Tali images.
++ = Can detect peak on histogram, but may be difficult to separate positive and negative peaks.
+++ = Easily detectable peaks on histogram. Positive and negative populations can be easily resolved.

Before you install

As a precaution when installing any update, save your vital data from the Tali Image-Based Cytometer:

Insert a USB drive into the Tali Image-Based Cytometer
Press the ‘Data’ tab at the top of the screen
Select the files you wish to save to the USB drive
Select Export format; choose either Data tables (.csv), Images (.jpg), Report (.pdf) or All formats
When the file(s) transfer is complete, remove the USB drive and transfer files to your computer

Note: If you experience system instability when exporting multiple files, all data are preserved. Turn the instrument off and then on to reinitialize the system.

Step 1—Download and save to a clean USB drive:

Download the current software v2.1 (release date 10/30/2012) and save it to a clean USB drive.

Download Tali software v2.1 (4.3 MB)

NOTE: Please contact Technical Support if you are updating from software version 1.0.6.5.

a. Press the download button and save the Tali Software zip file to your computer.
b. Obtain a USB Drive and remove all files or programs from this drive. Existing files can interfere with the software installation process thus it is important to remove all files or programs from the USB drive before continuing.
c. Using the clean USB drive, place the three programs and the image file from the zip file onto the USB drive.
d. After the transfer, review the file and program configuration of the USB Drive. The configuration should be the following (see below).

Step 2—Transfer files to your Tali® Image Cytometer

a. Insert the USB drive into the USB port located on the front of the Tali Instrument.
b. Press the ‘Settings’ tab (highlighted with the red box below).

c. Press the 'Update firmware' button (highlighted with the red box below).

d. Wait until the Tali instrument recognizes the USB drive and the green light is visible on the 'Update' button (see below).

e. Press the 'Update' button.
f. When the Update is complete the instrument will need to be restarted. Press the ‘OK’ button (see below).

g. After the instrument performs the restart, press the ‘Settings’ tab and check that the software has been updated to the latest version (see below).

Publications