A mixed population of live and isopropyl alcohol–killed Micrococcus luteus and Bacillus cereus stained with the LIVE/DEAD® BacLight™ Bacterial Viability Kit.

A mixed population of live and isopropyl alcohol–killed Micrococcus luteus and Bacillus cereus stained with the LIVE/DEAD® BacLight™ Bacterial Viability Kit (Cat. nos. L7007, L7012). Bacteria with intact cell membranes exhibit green fluorescence, whereas bacteria with damaged membranes exhibit red fluorescence. Prior to imaging, the bacteria were placed onto a polycarbonate filter and immersed in BacLight™ mounting oil. This multiple exposure image was acquired with a triple-bandpass optical filter set appropriate for simultaneous imaging of DAPI, fluorescein and Texas Red® dyes.

A mixed population of live and isopropyl alcohol–killed <i>Micrococcus luteus</i> and <i>Bacillus cereus</i> stained with the LIVE/DEAD® <i>Bac</i>Light™ Bacterial Viability Kit (Cat. nos. L7007, L7012). Bacteria with intact cell membranes exhibit green fluorescence, whereas bacteria with damaged membranes exhibit red fluorescence. Prior to imaging, the bacteria were placed onto a polycarbonate filter and immersed in <i>Bac</i>Light™ mounting oil. This multiple exposure image was acquired with a triple-bandpass optical filter set appropriate for simultaneous imaging of DAPI, fluorescein and Texas Red® dyes.

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