A double-labeled microsphere from the FocalCheck™ DoubleGreen Fluorescent Microsphere Kit.

A double-labeled microsphere from the FocalCheck™ DoubleGreen Fluorescent Microsphere Kit (Cat. no. F36905). The bead was imaged as a z-series using a Carl Zeiss LSM 510 META system. The two green-fluorescent dyes were separated by spectral unmixing, and one of the dyes was pseudocolored red. In this composite image, the complete z-series is shown prior to software rendering. Rendering fills in the missing information between the slices by interpolation to create a solid object.

A double-labeled microsphere from the FocalCheck™ DoubleGreen Fluorescent Microsphere Kit (Cat. no. F36905). The bead was imaged as a z-series using a Carl Zeiss LSM 510 META system. The two green-fluorescent dyes were separated by spectral unmixing, and one of the dyes was pseudocolored red. In this composite image, the complete z-series is shown prior to software rendering. Rendering fills in the missing information between the slices by interpolation to create a solid object.

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