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Vaginal Microbiota Research Testing Solutions
PCR-Based Research Use Only (RUO) Panels for vaginal infection testing
Detect microorganisms, faster and with higher accuracy
Research to better characterize and identify vaginal microbiota is important for the health of women, their fetuses, and newborns. Globally, there are approximately 1 billion women affected each year by urogenital infections, including vaginitis. Bacterial vaginosis alone affects 21.2 million women annually, and is the most common vaginal condition affecting women ages 15–44 [1]. This makes the detection of common vaginal pathogens important for clinical researchers to understand outbreaks and disease etiology.
Molecular tests are more reliable, accurate, and sensitive compared to traditional culture and microscopy methods. Real-time PCR can provide results in as little as 4–6 hours, whereas manual methods, such as culture, can take 3 days or longer [2,3].
The Applied Biosystems™ TaqMan™ OpenArray™ Vaginal Microbiota Comprehensive Plate employs real-time PCR technology, offering an end-to-end workflow for the detection of a broad range of vaginal infections by utilizing the power of Applied Biosystems QuantStudio real-time PCR systems.
Our range of Applied Biosystems TaqMan Assays gives you the flexibility and freedom to configure a highly accurate and scalable testing solution that is right for your lab.
Broad detection—research a wide range of microorganism targets associated with vaginal infections
Accurate and reproducible results—assays demonstrated to be highly accurate with reproducible performance in numerous tests for analytical sensitivity and specificity on different sample types
Scalable flexible workflow—real-time PCR and our variety of assay formats enable you to process tens to hundreds of samples per day on a single instrument
For Research Use Only. Not for use in diagnostic procedures.
Expand your vaginal microbiota research with real-time PCR
Broad detection of microorganisms
Real-time PCR can detect slow-growing or difficult-to-culture microorganisms, and can be used when traditional methods lead to ambiguous results. Utilizing real-time PCR along with microorganism-specific TaqMan Assays enable rapid and accurate detection and categorization of multiple microorganisms involved in vaginal microbiome composition and dynamics. Including many of these TaqMan Assays on a single research panel provides detection of the presence of multiple microorganisms and an indication of the overall status of vaginal microbiota.
TaqMan OpenArray Vaginal Microbiota Panel details
Bacteria | |
Atopobium vaginae Bacteroides fragilis BVAB2 Enterococcus faecalis Escherichia coli O1 Gardnerella vaginalis Lactobacillus crispatus Staphylococcus aureaus Ureaplasma urealyticum Lactobacillus gasseri Lactobacillus iners Lactobacillus jensenii Mobiluncus curtisii | Mobiluncus mulieris Streptococcus agalactiae (group B) Mycoplasma genitalium Mycoplasma hominis Prevotella bivia Uncultured Megasphaera 1 Uncultured Megasphaera 2 Haemophilus ducreyi Chlamydia trachomatis Neisseria gonorrhoeae Treponema pallidum |
Fungi |
Candida albicans Candida parapsilosis C Candida krusei Candida lusitaniae Candida glabrata Candida dubliniensis Candida tropicalis |
Viral |
Herpes simplex virus type 1 (HSV1) Herpes simplex virus type 2 (HSV2) |
Protozoan |
Trichomonas vaginalis |
Accurate and reproducible results
Applied Biosystems TaqMan assays for vaginal microorganisms have been tested to help ensure analytical performance has been achieved. Verification data demonstrates that our panel achieves excellent analytical accuracy, specificity, and sensitivity when tested against genomic DNAs (gDNAs) and synthetic controls so as to enable the success of your research studies.
Scalable qPCR workflow
The QuantStudio portfolio of instruments can meet any throughput requirement. For higher throughput laboratories, leverage our OpenArray nanofluidic technology. This technology allows labs to process up to 600 samples per day on a single instrument. A complete end-to-end solution is available and includes the Thermo Fisher Scientific KingFisher™ Flex benchtop automated DNA/RNA extraction instrument, convenient extraction and purification kits, and the OpenArray™ AccuFill™ System. Combined, these systems can automate sample and liquid handling to enable the processing of hundreds to thousands of research samples per day.
Whether you already own an instrument or are considering an expansion to your capabilities, your sample processing and throughput requirements can be addressed through the different formats compatible with your instrument (such as TaqMan array cards, 96- or 384-well plates, or single tube assays) with made-to-stock or custom panels. Learn more about how OpenArray technology can be an ideal solution for handling large numbers of samples.
Explore QuantStudio PCR Systems, a flexible and scalable real-time PCR platform. Learn more ›
For Research Use Only. Not for use in diagnostic procedures.
References
1. http://www.cdc.gov/std/bv/stats.htm
2. Finegold, David N. Genetic Diagnostic Technologies - Fundamentals. Merck Manuals Consumer Version, Merck Manuals, accessed 21 Feb 2023, https://www.merckmanuals.com/home/fundamentals/genetics/
3. Vazquez-Pertejo, Maria T. Diagnosis of Infectious Disease - Infections. Merck Manuals Consumer Version, Merck Manuals, accessed 21 Feb 2023, https://www.merckmanuals.com/home/infections/diagnosis-of-infectious-disease/diagnosis-of-infectious-disease.