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Download the Molecular Cloning Handbook

The molecular cloning workflow requires many steps to obtain the perfect clone for downstream applications. Advance your cloning workflow at every step—clone, transform, purify, and analyze to successfully produce a recombinant clone quickly.

This handbook is intended to guide you by providing technical information and clear choices across the cloning workflow with solutions designed for speed and simplicity. With all of our cloning solutions, you can find what fits and get the results you need to move closer to the next step in your discovery.

This handbook offers:

  • Considerations in sample preparation, reverse transcription, PCR, electrophoresis, cloning, and gene synthesis
  • Guidance on selecting the right molecular biology products for your research needs
  • Research tools to help you overcome common challenges in molecular biology workflow
  • Educational resources, technical tips, and troubleshooting guides

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Please indicate which cloning techniques you currently perform in your lab (check all that apply) *

 Traditional cloning (restriction enzymes & ligase-based)
 PCR TA & Blunt-end cloning (e.g., TOPO cloning)
 Recombination-based cloning (e.g., Gateway cloning)
 Seamless cloning (e.g., Gibson Assembly)
 Ligase independent cloning (LIC)
 Competent cells

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