Vanquish Inverse Gradient LC Systems

Improve your lab's quantification capabilities with the Thermo Scientific Vanquish Inverse Gradient LC Systems, employing the unique and universal Charged Aerosol Detection, to consistently quantify all non-volatile and semi-volatile compounds even when no reference standard is available.

Highlights

Universal detection

Vanquish Inverse Gradient LC Systems use the unique and versatile Charged Aerosol Detector (CAD), a universal detector for the sensitive measurement of non- and semi-volatile compounds – from small molecule to biomolecules, foods and beverages, specialty chemicals or polymers.

Uniform response for gradient elution

Changing organic composition over a gradient elution results in fluctuation in analyte response. Post column application of an inverse gradient compensation eliminates this effect by ensuring the eluent entering the detector always has the same solvent composition regardless of where the compound elutes during the gradient separation.

Standard-free quantification

Consistent detector response means that unknown substances can be quantified, even if you lack an individual standard of a specific compound.

Simplified method setup

Effortlessly apply an inverse gradient with Thermo Scientific Chromeleon Chromatography Data System (CDS) wizards that take all system volumes into account and automatically calculate all required parameters for you.

Easy and reliable tool-free connections

The fluidic pathway of the Vanquish Inverse Gradient LC Systems are fitted with Thermo Scientific Viper Fingertight Fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software

The Vanquish Inverse Gradient LC Systems seamlessly integrate with our Chromeleon CDS software to deliver superior compliance tools, networking capabilities, automation and data processing.

What is inverse gradient liquid chromatography?

The inverse gradient system allows you to consistently quantify all non-volatile and semi-volatile compounds using universal Charged Aerosol Detection.

Quantification recovery of unknown compounds

For a gradient separation, the quantification recovery of unknown compounds with CAD is best with the eluting conditions from the Inverse Gradient approach. For a standard CAD, the quantification recovery will depend on the organic content. UV detection recovery depends on extinction coefficient of the analyte.

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System specifications

The table below provides an overview of the Vanquish Inverse Gradient LC Systems specifications for a standard setup. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category	Vanquish Inverse Gradient LC Systems
System pressure, maximum	700, 1000 or 1500 bar, depends on system configuration
Flow range	0-5, 0-8, 0-10 mL/min, depends on system configuration
Pump options (default gradient delay volume)	Binary (35, 200, or 400 µL) Quaternary (679 µL) Dual (679 µL) Delay volume depends on pump(s) selected
Biocompatible	Yes, depends on system configuration
pH range	2–12, chloride concentration up to 1 mol/L
System design	Modular
Touchscreen user interface display compatible (optional)	Yes, depends on system configuration
Solvent monitoring system compatible (optional)	Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer	Yes, depends on system configuration
SmartInject technology for maximum injection precision and improved column lifetime	Yes
Injection volume range	Standard: Up to 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon) Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core) Depends on system configuration
Sample cooling	Yes, 4-40°C Depends on sampler selected
Sample capacity (standard) Based on standard 12 mm OD vials (≤ 1.5 mL)	4 racks of 54 vials (≤ 1.5mL) each, other rack formats available + capacity of 12 x 22.5 mm OD vials (≤ 10 mL) in the carousel
Sampler extension available (optional)	Yes, with Vanquish Charger
Column compartment thermostatting	Yes, 5-120°C, depends on system configuration Still air and forced air
Column compartment pre-heater	Active and passive Depends on system configuration
Column compartment post-column cooler	Yes Depends on column compartment selected
Detector choices	Vanquish Charged Aerosol Detector Optional add-on detectors: Vanquish Diode Array Detector Vanquish Multiple Wavelength Detector Vanquish Variable Wavelength Detector

Component specifications

Standard Vanquish Duo HPLC and UHPLC Systems for Inverse Gradient LC are made up of the following components. More detail is available below.

	Vanquish Horizon UHPLC System	Vanquish Flex Inverse Gradient UHPLC System	Vanquish Core Inverse Gradient HPLC System
Pump Download Specification Sheet	2 Vanquish Binary Pump H	2 Vanquish Binary Pump F or 2 Vanquish Quaternary Pump F or 1 Vanquish Dual Pump F	2 Vanquish Binary Pump C/CN or 2 Vanquish Quaternary Pump C/CN or 1 Vanquish Dual Pump C/CN
Autosampler Download Specification Sheet	Vanquish Split Sampler HT	Vanquish Split Sampler FT	Vanquish Split Sampler C or Vanquish Split Sampler CT
Extended sample capacity (optional) Download Specification Sheet	Vanquish Charger  (optional)
Column compartment Download Specification Sheet	Vanquish Column Compartment H		Vanquish Column Compartment C
Virtually zero dead volume system connections Download Specification Sheet	Viper UHPLC Fittings Kits
Detectors	Charged Aerosol Detector For additional detectors, visit our HPLC and UHPLC Detectors page

Vanquish Inverse Gradient LC Systems