Qubit 4 Fluorometer | Thermo Fisher Scientific

Fast, accurate DNA, RNA, and protein quantification for a single sample

The Invitrogen Qubit 4 Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in a single sample. It can also be used to assess RNA integrity and quality. The easy-to-use touchscreen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Looking for higher throughput? Our Qubit Flex Fluorometer can quantify up to eight samples at a time

Features

The Qubit 4 Fluorometer shares common features with the Qubit Flex model: It is accurate, specific, sensitive, simple, and fast; it requires as little as 1 µL of sample, even if highly diluted; it offers an onboard Reagent Calculator to help in preparing your working solution; it works with a broad range of Qubit Assays and applications; and it can measure raw fluorescence in Fluorometer mode.

In addition, data gathered in Fluorometer mode on the Qubit 4 Fluorometer can be used to design your own assay using the MyQubit assay design tool. This tool lets you create new assays for the Qubit Fluorometer in minutes. Simply enter your assay parameters into the online tool, and then save and upload the .qbt file to your Qubit Fluorometer using a USB drive.

Preconfigured MyQubit assays to quantify cholesterol, galactose, glucose, glutamic acid, peroxide, or sucrose are available. You can download these .qbt files to your computer and then upload them to your Qubit 4 Fluorometer using a USB drive. These are examples of the kinds of MyQubit assays you can create on your own.

Learn more about MyQubit assays

Simple workflow

The user-friendly Qubit 4 Fluorometer delivers results using quick, simple assay procedures. The large, color touch screen allows you to easily navigate through the various options. Calculations and settings are automatically performed by the instrument.

Simple workflow

To quantify a sample with the Qubit 4 Fluorometer, select an assay, select the output, press Read tube, and view the results.

The Qubit 4 Fluorometer will save the data for up to 1,000 samples, and you can transfer data to a Thermo Fisher Connect Cloud account via WiFi, or to a computer via a USB drive or cable. In addition, the Qubit 4 integrated Reagent Calculator helps you determine the amount of dye and buffer required to prepare your samples and standards.

Reagent Calculator and results graph. Enter the number of samples and standards needed and the built-in Reagent Calculator will determine the amount of dye and buffer needed. The results can be viewed on a graph showing the standard curve.

View how-to videos for the Qubit 4 Fluorometer

Specifications

Attribute	Description
Physical characteristics
Instrument type	Benchtop fluorometer
Instrument dimensions (W x L x H)	13.6 x 25 x 5.5 cm 5.4 x 10 x 2.2 in.
Weight	743 g; 2.1 lb
Operating power	100-240 VAC, 1.0 A/1.2 A
Frequency	50/60 Hz
Electrical input	12 VDC, 2.5 A
Installation site	Indoor use only
Operating temperature	10-40° C
Operating humidity	20-80% (non-condensing)
User interface	5.7-inch color touchscreen
Technical specifications
Dynamic range	5 orders of magnitude
Processing time	<5 seconds/sample
Light sources	Blue LED (peak ~470 nm) Red LED (peak ~635 nm)
Excitation filters	Blue LED (430–495 nm) Red LED (600–645 nm)
Emission filters	Green (510–580 nm) Red (665–720 nm)
Detectors	Photodiodes: measurement capability from 300–1,000 nm
Calibration type	2- or 3-point standard
Sample chamber	Accommodates one Qubit Assay Tube
Tube type	Qubit Assay Tube
Warm-up time	<35 seconds
Sample data storage	1,000 samples
Hardware
Display	5.7 in. capacitive touchscreen with high-resolution color display
Data ports	USB cable port (type mini-B) USB drive port (type A)
Networking capabilities	Transfer data directly to computer using a USB cable or wirelessly using the supplied Wi-Fi adaptor
Power supply	AC adapter with country-specific power cords
USB drive	4 GB

Ordering information

Starter kit comparison guide

Qubit 4 Fluorometer	Qubit 4 Quantitation Starter Kit	Qubit 4 NGS Starter Kit	Qubit 4 RNA IQ Starter Kit	Qubit 4 Protein Broad Range Starter Kit

Qubit 4 Fluorometer	Qubit 4 Fluorometer	Qubit 4 Fluorometer	Qubit 4 Fluorometer	Qubit 4 Fluorometer
1 international power adapter	1 international power adapter	1 international power adapter	1 international power adapter	1 international power adapter
Microfiber cloth	Microfiber cloth	Microfiber cloth	Microfiber cloth	Microfiber cloth
USB drive	USB drive	USB drive	USB drive	USB drive
WiFi dongle	WiFi dongle	WiFi dongle	WiFi dongle	WiFi dongle
	Qubit Assay Tubes (500 tubes)	Qubit Assay Tubes (500 tubes)
	Qubit dsDNA HS (high sensitivity) Assay Kit (100 assays)	Qubit dsDNA HS (high sensitivity) Assay Kit (500 assays)
	Qubit dsDNA BR (broad range) Assay Kit (100 assays)
	Qubit RNA IQ (integrity and quality) Assay Kit (75 assays)		Qubit RNA IQ (integrity and quality) Assay Kit (75 assays)
	Qubit RNA HS (high sensitivity) Assay Kit (100 assays)
	Qubit RNA BR (broad range) Assay Kit (100 assays)
	Qubit Protein BR Assay Kit (100 assays)			Qubit Protein BR Assay Kit (100 assays)
	Qubit 4 System Verification Assay Kit (50 assays)
Q33238	Q33239	Q33240	Q33241	A51292

For information about assays for the Qubit 4 Fluorometer, see the Assays tab.

For Research Use Only. Not for use in diagnostic procedures.