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Cell Culture Flasks
Cell culture flask feature guide
Thermo Scientific cell culture flasks are designed for consistency, cell health, and reproducibility. Nunc flasks offer surface and ancillary options for your research needs.
Surface treatments include Nunclon Sphera, an ultra-low binding surface for spheroid cultures, and poly-D-lysine and collagen I for cells with low adherence or growth.
NEW Nunclon Supra™ Surfaces for advanced cell culturing
We are proud to provide a new product line surface treatment that will support improved cell yield and morphology as well enabling you to do xeno-free or low-serum applications.
Order yours today: Dishes | Flasks | Plates | 96-well plates
See how our surfaces perform with 39 cell types and cell lines at thermofisher.com/supradata
What makes Nunc EasYFlask products so easy to use?
Ergonomic and optically clear thanks to their virgin polystyrene construction, EasYFlask products are designed for convenience. EasYFlask products are offered as either non-treated flasks or Nunclon Delta surface treated flasks, optimized for both adherent cells and suspension cells respectively.
| Why EasYFlask products are the optimal choice for cell culture and scale-up | |
|---|---|
| Neck width | Extra wide neck provides easier access to growth surface with scraper or pipette |
| Cap opening | 1/3-turn cap enables one-handed operation and avoids wrist strains |
| Volume graduations | Molded and printed for easy and quick measurement of growth media |
| Sterility assurance level (SAL) | Sterility assurance of SAL 10-6 guarantees that no more than one flask out of one million may be non-sterile |
| Lot-to-lot testing and certification in each case | Lot specific testing across four cell lines and primary cells ensures consistency, reproducibility, and reliability of quality; certificate included in each case |
| Airflow below flask | Vented ribs on flask bottom enable optimal temperature equilibration |
| Robust design | Thick walls provide robust design for better resistance to mechanical stress |
| Continuity of surface for scale up | The Thermo Scientific Nunclon Delta surface—for maximum attachment and growth of adherent cultures—is offered across our T25, T75, T175, T225, and T500 flasks, enabling consistency and confidence when scaling up |
Available cell culture flask sizes
T25–T225 cell culture flasks
Choose flasks from either straight or angled-neck collections, available in Nunclon Delta-, Nunclon Sphera-, poly-D-lysine-, collagen I-surface treatments or untreated surface varieties.
Explore T25 flasks
Available in untreated, Nunclon Delta, Nunclon Sphera, poly-D-lysine, and collagen I surfaces.
Explore T75 flasks
Available in untreated, Nunclon Delta, Nunclon Sphera, poly-D-lysine, and collagen I surfaces.
Explore T175 flasks
Available in untreated, Nunclon Delta, poly-D-lysine, and collagen I surfaces.
Explore T225 flasks
Available in untreated, Nunclon Delta, poly-D-lysine, and collagen I surfaces, as well as wide-mouthed openings.
Nunc TripleFlask products
Choose a multilayer TripleFlask product for 500cm2 of surface area in a T175 footprint. Surface treatment options include untreated surfaces and Nunclon Delta treatments.
Shake flasks
PETG Erlenmeyer flasks are ideal for suspension of cell culture. Vent cap for continuous gas exchange while maintaining sterility and preventing leakage.
Large-scale tissue culture flasks
T300 cell culture flasks
Choose a T300 flask with a total volume of 1,900 mL, the largest single layer cell culture treated flask on the market. This flask includes a large writing area, prominent stacking feet, and a filter cap with 0.2 µm pore-sized hydrophobic membrane for sufficient gas exchange.
T500 cell culture flasks
Perform cell culture expansion and experience 500 cm2 total growth area within the footprint of a standard flask by way of the three parallel layers and a straight neck design that works effectively within automation systems.
Cell factory systems
Nunc cell factory systems are a proven solution for large-scale production of cells, vaccines, and therapeutic proteins. Available in 1- to 40-layer formats, these systems enhance productivity for scale-up.
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For Research Use Only. Not for use in diagnostic procedures.